中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (12): 1102-1106.doi: 10.12007/j.issn.0258-4646.2018.12.011

• 论著 • 上一篇    下一篇

去酰基化ghrelin抑制上皮性卵巢癌细胞的增殖

窦磊1, 庞晓燕1, 李奇1, 尚海2, 张颐1   

  1. 1. 中国医科大学附属第一医院妇科, 沈阳 110001;
    2. 辽宁省肿瘤医院肝胆胰外科, 沈阳 110042
  • 收稿日期:2018-09-30 出版日期:2018-12-30 发布日期:2018-12-06
  • 通讯作者: 张颐 E-mail:syzi@163.com
  • 作者简介:窦磊(1984-),男,主治医师,硕士.
  • 基金资助:
    沈阳市科技计划(17-231-1-54)

Des-Acyl Ghrelin Inhibits the Proliferation of Ovarian Epithelial Carcinoma Cells

DOU Lei1, PANG Xiaoyan1, LI Qi1, SHANG Hai2, ZHANG Yi1   

  1. 1. Department of Gynecology, The First Hospital, China Medical University, Shenyang 110001, China;
    2. Department of Hepatobiliary Surgery, Liaoning Tumor Hospital & Institute, Shenyang 110042, China
  • Received:2018-09-30 Online:2018-12-30 Published:2018-12-06

摘要: 目的 探讨去酰基化ghrelin(DAG)对上皮性卵巢癌细胞增殖的作用及其相关分子机制。方法 培养上皮性卵巢癌细胞SKOV3,以DAG处理,并予以激动剂干预哺乳动物雷帕霉素靶蛋白(mTOR)和经典Wnt信号途径,采用CCK-8法测定细胞增殖。结果 DAG呈剂量依赖性抑制卵巢癌SKOV3细胞的增殖。DAG抑制mTOR信号途径,抑制经典Wnt信号转录活性以及下游靶基因的mRNA水平,mTOR和Wnt信号激活可在一定程度上翻转DAG对SKOV3细胞增殖的抑制作用。结论 DAG通过抑制mTOR和经典Wnt信号通路发挥其抑制卵巢癌细胞增殖的作用,为卵巢癌防治提供新思路。

关键词: 去酰基化ghrelin, 上皮性卵巢癌, 增殖, mTOR信号途径, Wnt信号途径

Abstract: Objective To assess the effect of des-acyl ghrelin (DAG) on the proliferation of ovarian epithelial carcinoma cells and the related mechanism. Methods SKOV3 ovarian epithelial carcinoma cells were cultured and treated with DAG,with or without mammalian target of rapamycin (mTOR) and Wnt signaling pathway activator. Cell proliferation was measured by the CCK-8 assay. Results DAG inhibited the proliferation and growth of SKOV3 cells in a dose-dependent manner. DAG exposure induced the inhibition of the mTOR and Wnt signaling pathways. Activation of these two pathways blocked the inhibition of SKOV3 cell proliferation by DAG. Conclusion DAG inhibits the proliferation of SKOV3 ovarian epithelial carcinoma cells through the mTOR and Wnt signaling pathways,and may contribute to prevention and therapy of ovarian epithelial carcinoma.

Key words: des-acyl ghrelin, ovarian epithelial carcinoma, proliferation, mTOR signaling pathway, Wnt signaling pathway

中图分类号: 

  • R737.31
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