中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2019, Vol. 48 ›› Issue (1): 17-22,28.doi: 10.12007/j.issn.0258-4646.2019.01.004

• 论著 • 上一篇    下一篇

miR-200c调控肽基脯氨酰顺反异构酶对喉癌Hep-2细胞生物学行为的影响

温行1, 李章富1, 王辉1, 孙绍华1, 郭星2, 李福才1   

  1. 1. 中国医科大学基础医学院医学遗传学教研室, 沈阳 110122;
    2. 中国医科大学附属第一医院耳鼻喉科, 沈阳 110001
  • 收稿日期:2018-06-14 出版日期:2019-01-30 发布日期:2019-01-05
  • 通讯作者: 李福才 E-mail:fcli@cmu.edu.cn
  • 作者简介:温行(1993-),女,硕士研究生.
  • 基金资助:
    国家自然科学基金(81272969)

Effect of miR-200c Regulation of Peptidyl-prolyl Cis/trans Isomerase on the Biological Behavior of Hep-2 Cells

WEN Xing1, LI Zhangfu1, WANG Hui1, SUN Shaohua1, GUO Xing2, LI Fucai1   

  1. 1. Department of Medical Genetics, College of Basic Medical Sciences, China Medical University, Shenyang 110122, China;
    2. Department of Otolaryngology, The First Hospital, China Medical University, Shenyang 110001, China
  • Received:2018-06-14 Online:2019-01-30 Published:2019-01-05

摘要: 目的 探究miR-200c对喉癌Hep-2细胞生物学行为的影响以及miR-200c是否通过肽基脯氨酰顺反异构酶(PIN1)在喉癌中发挥其生物学功能。方法 实时PCR检测喉癌组织中miR-200c的表达水平;miR-200c相关小RNAs瞬时转染喉癌Hep-2细胞,Transwell实验检测细胞的迁移能力,免疫荧光实验检测细胞中心体的异常扩增;双荧光素酶报告基因系统检测miR-200c与PIN1的结合;Western blotting检测PIN1蛋白的表达水平。结果 miR-200c在喉癌组织中的表达水平显著增高;Transwell结果显示,miR-200c能够抑制Hep-2细胞的迁移,免疫荧光实验显示,miR-200c能够减弱细胞中心体的异常扩增;双荧光素酶报告基因结果证实,PIN1是miR-200c的靶基因之一;Western blotting结果显示,miR-200c可在翻译水平抑制PIN1的表达;miR-200c能够通过调控PIN1PIN1抑制喉癌细胞的迁移能力和减弱细胞中心体的异常扩增。结论 miR-200c通过调控PIN1抑制喉癌细胞的迁移能力,并减弱中心体异常扩增。

关键词: 喉癌, miR-200c, 肽基脯氨酰顺反异构酶, 迁移, 中心体

Abstract: Objective To explore the influence of miR-200c on the biological behavior of laryngeal carcinoma Hep-2 cells and determine whether miR-200c exerts its biological function through peptidyl-prolyl cis/trans isomerase (PIN1) in laryngeal carcinoma. Methods A qRT-PCR assay for the expression of miR-200c was performed in laryngeal carcinoma tissues. Hep-2 cells were transfected with miR-200c related small RNAs. Transwell assay detected the migration ability of the cells. Immunofluorescence assay was used to detect the abnormal amplification of the centrosome. A dual luciferase reporter gene system was used to detect the binding ability between miR-200c and PIN1. Western blotting detected the protein expression level of PIN1. Results The expression of miR-200c in laryngeal carcinoma was significantly increased. miR-200c inhibited the migration of Hep-2 cells and could weaken the abnormal amplification of centrosome. PIN1 was confirmed as one of the target genes of miR-200c. miR-200c inhibited the expression of PIN1 at the translation level and could inhibit Hep-2 cell migration and abnormal centrosome amplification by regulating PIN1. Conclusion miR-200c can inhibit the migration ability of laryngeal carcinoma cells and abnormal centrosome amplification by regulating PIN1.

Key words: laryngeal squamous cell carcinoma, miR-200c, peptidyl-prolyl cis/trans isomerase, migration

中图分类号: 

  • R394
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