中国医科大学学报

中国医科大学学报
  • 中文核心期刊
  • 中国科技核心期刊
  • 中国高校百佳科技期刊
  • BA、CA收录

中国医科大学学报 ›› 2019, Vol. 48 ›› Issue (1): 66-70.doi: 10.12007/j.issn.0258-4646.2019.01.014

• 论著 • 上一篇    下一篇

那可丁对人肺癌细胞A549生物学功能的影响

李岩, 张德巍, 杨大业   

  1. 中国医科大学附属第四医院第三普通外科, 沈阳 110032
  • 收稿日期:2017-11-09 出版日期:2019-01-30 发布日期:2019-01-05
  • 通讯作者: 杨大业 E-mail:yangdaye73@126.com
  • 作者简介:李岩(1987-),男,医师,硕士.

Effect of Noscapine on the Biological Function of A549 Cells

LI Yan, ZHANG Dewei, YANG Daye   

  1. Third General Surgery Department, The Fourth Affiliated Hospital, China Medical University, Shenyang 110032, China
  • Received:2017-11-09 Online:2019-01-30 Published:2019-01-05

摘要: 目的 探讨那可丁对人肺癌细胞系A549细胞生物学功能的影响及其作用机制。方法 使用那可丁(10 μmol/L和20 μmol/L)作用于A549细胞,通过MTT观察那可丁对细胞增殖的影响;通过Western blotting和实时PCR检测那可丁作用于A549细胞后Cyclin D1、bcl-2、MMP2蛋白和mRNA的表达;Hoechst 33258染色检测那可丁对A549细胞凋亡的影响;Transwell检测那可丁对细胞转移的影响。结果 MTT检测发现10、20 μmol/L那可丁分别作用于A549细胞24 h后对细胞增殖抑制率分别为(32.98±1.09)%和(49.56±3.98)%,差异有统计学意义(P<0.05)。Hoechst 33258染色显示那可丁促进细胞凋亡,Transwell实验显示那可丁抑制A549细胞转移。Western blotting和实时PCR结果显示那可丁抑制A549细胞中Cyclin D1、bcl-2和MMP2的表达。结论 那可丁能够抑制人肺癌细胞系A549细胞生长及转移、促进A549细胞凋亡,可以作为治疗肺癌的潜在药物。

关键词: 那可丁, A549, 增殖, 凋亡, 转移

Abstract: Objective To explore the effect of noscapine on the biological function of A549 cells and its underlying mechanism. Methods Concentrations of 10 μmol/L and 20 μmol/L of noscapine were used on the A549 cells. The effect of noscapine on their proliferation was observed via the MTT assay. Western blotting and real time PCR were used to detect the effects on Cyclin D1,BCL-2,and MMP2. The Hoechst 33258 staining assay was used to observe the effects of noscapine on the apoptosis of A549 cells. The metastasis of A549 cells was detected by using the Transwell assay. Results The MTT assay showed that the rate of inhibition of cell proliferation after treatment with 10 μmol/L and 20 μmol/L noscapine were (32.98±1.09)% and (49.56±3.98)%,with a significant difference between the values (P<0.05). Hoechst 33258 staining evinced that noscapine promoted apoptosis,while the Transwell assay displayed that noscapine inhibited A549 cell metastasis. Western blotting and real time PCR indicated that the expression of Cyclin D1,BCL-2,and MMP2 in A549 cells was inhibited by noscapine. Conclusion Noscapine can inhibit the growth and metastasis of the lung cancer cell line A549 and promote the apoptosis of A549 cells. In conclusion,noscapine can potentially be used as a chemotherapeutic drug for lung cancer.

Key words: noscapine, A549, proliferation, apoptosis, metastasis

中图分类号: 

  • R734.2
[1] LEE JH,LEEM DG,CHUNG KS,et al. Panaxydol derived from Panax ginseng inhibits G1 cell cycle progression in non-small cell lung cancer via upregulation of intracellular Ca2+ levels[J]. Biol Pharm Bull,2018,41(11):1701-1707. DOI:10.1248/bpb.b18-00447.
[2] OGATA T,KATSUI K,YOSHIO K,et al. Dose-volume parameters predict radiation pneumonitis after surgery with induction concurrent chemoradiotherapy for non-small cell lung cancer[J]. Acta Med Okayama,2018,72(5):507-513. DOI:10.18926/AMO/56249.
[3] METHER RK,NAIK MR J,BASTIA B,et al. Comparative evaluation of anti-angiogenic effects of noscapine derivatives[J]. Bioinformation,2018,31(5):236-240. DOI:10.6026/97320630014236.
[4] CRUIJSEN M,HOBO W,VAN DER VELDEN WJ,et al. Addition of 10-day noscapine to fludarabine/total body irradiation conditioning is feasible and induces tumor-associated antigen-specific T cell responses[J]. Biol Blood Marrow Transplant,2016,22(6):1000-1008. DOI:10.1016/j.bbmt.2016.02.003.
[5] DODDAPANENI R,PATEL K,CHOWDHURY N,et al. Reversal of drug-resistance by noscapine chemo-sensitization in docetaxel resistant triple negative breast cancer[J]. Sci Rep,2017,7(1):15824. DOI:10.1038/s41598-017-15531-1.
[6] TIAN X,LIU M,ZHU Q,et al. Down-regulation of liver-intestine cadherin enhances noscapine-induced apoptosis in human colon cancer cells[J]. Expert Rev Anticancer Ther,2017,17(9):857-863. DOI:10.1080/14737140.2017.1344097.
[7] SONI N,JYOTI K,JAIN UK,et al. Noscapinoids bearing silver nanocrystals augmented drug delivery,cytotoxicity,apoptosis and cellular uptake in B16F1,mouse melanoma skin cancer cells[J]. Biomed Pharmacother,2017,90:906-913. DOI:10.1016/j.biopha.2017.04.042.
[8] GHALY PE,ABOU El-MAGD RM,CHURCHILL CD,et al. A new antiproliferative noscapine analogue:chemical synthesis and biological evaluation[J]. Oncotarget,2016,7(26):40518-40530. DOI:10.18632/oncotarget.9642.
[9] SHEN W,LIANG B,YIN J,et al. Noscapine increases the sensitivity of drug-resistant ovarian cancer cell line SKOV3/DDP to cisplatin by regulating cell cycle and activating apoptotic pathways[J]. Cell Biochem Biophys,2015,72(1):203-213. DOI:10.1007/s12013-014-0438-y.
[10] MAHADDALKAR T,NAIK PK,CHOUDHARY S,et al. Structural investigations into the binding mode of a novel noscapine analogue,9-(4-vinylphenyl) noscapine,with tubulin by biochemical analyses and molecular dynamic simulations[J]. J Biomol Struct Dyn,2017,35(11):2475-2484. DOI:10.1080/07391102.2016.1222969.
[11] QUISBERT-VALENZUELA EO,CALAF GM. Apoptotic effect of noscapine in breast cancer cell lines[J]. Int J Oncol,2016,48(6):2666-2674. DOI:10.3892/ijo.2016.3476.
[12] DODDAPANENI R,PATEL K,CHOWDHURY N,et al. Noscapine chemosensitization enhances docetaxel anticancer activity and nanocarrier uptake in triple negative breast cancer[J]. Exp Cell Res,2016,346(1):65-73. DOI:10.1016/j.yexcr.2016.05.006.
[13] XU G,NIU Z,DONG J,et al. Noscapine inhibits human hepatocellular carcinoma growth through inducing apoptosis in vitro and in vivo[J]. Neoplasma,2016,63(5):726-733. DOI:10.4149/neo_2016_509.
[14] WU DM,DENG SH,LIU T,et al. TGF-β-mediated exosomal lnc-MMP2-2 regulates migration and invasion of lung cancer cells to the vasculature by promoting MMP2 expression[J]. Cancer Med,2018,7(10):5118-5129. DOI:10.1002/cam4.1758.
[15] CHEN Z,JI N,WANG Z,et al. Fine particulate matter (PM2.5) promoted the invasion of lung cancer cells via an ARNT2/PP2A/STAT3/MMP2 pathway[J]. J Biomed Nanotechnol,2018,14(12):2172-2184. DOI:10.1166/jbn.2018.2645.
[1] 樊璐, 王慧, 肖庆桓. 跨膜蛋白16F对乳腺癌细胞增殖及迁移的影响[J]. 中国医科大学学报, 2019, 48(2): 124-127.
[2] 王黎明, 杨大业, 仇剑, 张德巍. miR-24对肺癌生物学功能的抑制作用[J]. 中国医科大学学报, 2019, 48(1): 71-74.
[3] 刘松, 齐放, 赵宇, 陈克研, 张升瑞. 青蒿琥酯对人结肠癌细胞侵袭转移及TGF-β1/Smad4信号通路表达的影响[J]. 中国医科大学学报, 2019, 48(1): 34-38.
[4] 曹勇, 王东旭, 孙妍, 杨俊, 张营, 郑长清. miR-26a在溃疡性结肠炎中的表达及对自噬的影响[J]. 中国医科大学学报, 2018, 47(9): 807-811.
[5] 岳媛, 范秋灵, 都姝妍, 远方, 徐莉, 李琳, 刘楠, 姜奕, 王力宁. 乌索酸对高糖诱导人系膜细胞损伤的作用[J]. 中国医科大学学报, 2018, 47(8): 682-686.
[6] 张怡, 林冰, 陈剑锋. 人脐带干细胞治疗重度烧伤大鼠急性肝损伤的实验研究[J]. 中国医科大学学报, 2018, 47(8): 687-691.
[7] 宋起滨, 王哲, 王宇令, 李杨, 林爽. 脂肪源性干细胞促进大鼠神经施万细胞增殖和分泌功能[J]. 中国医科大学学报, 2018, 47(6): 527-531.
[8] 李忠华, 李慧峰, 李晓蕾. 瞬时感受器电位香草酸受体3促进胶质瘤U251细胞系增殖和侵袭[J]. 中国医科大学学报, 2018, 47(5): 394-397.
[9] 马刚, 刘宁, 徐亮, 刘哲, 宋从浩, 王丹璞. 程序性细胞死亡因子4与5-氟尿嘧啶对胰腺癌细胞凋亡的协同作用[J]. 中国医科大学学报, 2018, 47(5): 431-434.
[10] 范一博, 车晓芳, 赵欢, 胡雪君, 曲秀娟, 刘云鹏. microRNA-1183通过抑制CBL-B的表达促进胃癌细胞的增殖及转移[J]. 中国医科大学学报, 2018, 47(3): 202-205,211.
[11] 张苏宁, 刘宗昂. 小细胞肺癌中DBC1的表达及预后相关性研究[J]. 中国医科大学学报, 2018, 47(3): 222-225.
[12] 宫雪, 于柳, 祝兴旺, 刘嘉, 刘屹立, 王平. 抑制Rab11表达对人膀胱癌细胞增殖和侵袭的影响[J]. 中国医科大学学报, 2018, 47(3): 247-250,259.
[13] 于婷, 王小梅. 通心络对高糖培养下心肌成纤维细胞增殖和凋亡的影响[J]. 中国医科大学学报, 2018, 47(2): 132-136.
[14] 杜国强, 王瑾, 柳斌. 扁桃体滤泡树突状细胞肉瘤伴转移1例报告及文献复习[J]. 中国医科大学学报, 2018, 47(2): 180-182.
[15] 窦磊, 庞晓燕, 李奇, 尚海, 张颐. 去酰基化ghrelin抑制上皮性卵巢癌细胞的增殖[J]. 中国医科大学学报, 2018, 47(12): 1102-1106.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] 刘晓默, 张梦琦, 林久祥. 正畸托槽与弓丝间摩擦力性能的实验研究[J]. 中国医科大学学报, 2019, 48(1): 23 -28 .
[2] 张海鑫, 苏琪. 中性粒细胞与淋巴细胞比值在Ⅱ、Ⅲ期中低位直肠癌评估中的临床价值[J]. 中国医科大学学报, 2019, 48(1): 75 -77,84 .
[3] 封宇, 刘颖, 阎雪晶, 欧阳嶷. 双侧颞极白质受累的神经系统遗传疾病12例临床诊断分析[J]. 中国医科大学学报, 2019, 48(1): 90 -93 .
[4] 翟晴, 田阿勇. 富血小板血浆分离应用于体外循环心脏手术的研究进展[J]. 中国医科大学学报, 2019, 48(2): 180 -183 .
[5] 张鑫, 李小寒. 护士循证护理综合能力影响因素分析[J]. 中国医科大学学报, 2019, 48(1): 53 -57 .
[6] 许勇民, 孙大壮, 宋春青, 王蕊, 董雪松. MnTMPyP通过抑制氧化应激和内质网应激减轻百草枯致肺上皮细胞损伤[J]. 中国医科大学学报, 2019, 48(1): 1 -6 .
[7] 兰翀, 聂小毳, 史玉林, 徐洪涛. TC1和β-连环蛋白在宫颈癌和癌前病变组织中表达的关系和意义[J]. 中国医科大学学报, 2019, 48(1): 7 -11 .
[8] 兰雨桐, 刘维新, 来爽, 邓秋萍, 杨美琪, 陈轶楠. 小肠结节性淋巴滤泡增生症1例[J]. 中国医科大学学报, 2019, 48(2): 187 -189 .
[9] 冯颜, 潘秋亚, 项阳, 李静. 自身免疫性甲状腺炎血清蛋白质二硫化物异构酶A3抗体的表达[J]. 中国医科大学学报, 2019, 48(1): 12 -16 .
[10] 温行, 李章富, 王辉, 孙绍华, 郭星, 李福才. miR-200c调控肽基脯氨酰顺反异构酶对喉癌Hep-2细胞生物学行为的影响[J]. 中国医科大学学报, 2019, 48(1): 17 -22,28 .

中国医科大学学报版权所有©2018

未经允许,严禁擅自转载本站图文资料

地址:中国 沈阳市沈北新区蒲河路77号 110122

辽ICP备05014850

JOURNAL OF CHINA MEDICAL UNIVERSITY

ADDRESS: NO.77 PUHE ROAD

SHENYANG NORTH NEW AREA, SHENYANG

LIAONING PROVINCE, P.R. CHINA