中国医科大学学报

中国医科大学学报
  • 中文核心期刊
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中国医科大学学报 ›› 2019, Vol. 48 ›› Issue (1): 71-74.doi: 10.12007/j.issn.0258-4646.2019.01.015

• 论著 • 上一篇    下一篇

miR-24对肺癌生物学功能的抑制作用

王黎明, 杨大业, 仇剑, 张德巍   

  1. 中国医科大学附属第四医院第三普通外科, 沈阳 110032
  • 收稿日期:2017-11-14 出版日期:2019-01-30 发布日期:2019-01-05
  • 通讯作者: 张德巍 E-mail:421310907@qq.com
  • 作者简介:王黎明(1985-),男,医师,硕士.

miR-24 Inhibits Lung Cancer Cell Proliferation and Migration

Wang Liming, Yang Daye, Qiu Jian, Zhang Dewei   

  1. Third general surgery Department, The Fourth Affiliated Hospital, China Medical University, shenyang 110032, China
  • Received:2017-11-14 Online:2019-01-30 Published:2019-01-05

摘要: 目的 探讨miR-24调节肺癌发生发展的机制。方法 实时PCR检测30例肺癌组织中miR-24的含量,分析miR-24与患者生存期的关系。肺癌细胞系A549细胞中分别过表达或沉默miR-24后,通过MTT实验观察miR-24对其增殖的影响。Transwell实验观察miR-24对其迁移能力的影响。Western blotting检测miR-24对于A549细胞中增殖和迁移相关蛋白(CDK4/6、MMP2)的影响。结果 生存分析发现,miR-24表达低的患者5年生存率较低。MTT检测显示,miR-24过表达后可以显著抑制A549细胞的增殖,反之,当miR-24受到抑制后A549细胞的增殖受到促进。Transwell实验显示,miR-24过表达后可以显著抑制A549细胞的迁移,反之,当miR-24受到抑制后A549细胞的迁移受到促进。Western blotting检测结果显示,过表达miR-24可以抑制A549细胞中CDK4/6和MMP2的表达,miR-24受到抑制后A549细胞中CDK4/6和MMP2的表达受到促进。结论 miR-24可以抑制肺癌细胞的增殖和迁移。

关键词: miR-24, 肺癌, A549, 增殖, 迁移

Abstract: Objective To investigate how microRNA 24 (miR-24) regulates the development of lung cancer. Methods The levels of miR-24 in 30 cases of lung cancer were detected using real-time PCR,and the relationship between miR-24 levels and overall survival was analyzed. After overexpression or silencing of miR-24 in A549 lung cancer cells,the effect on cell proliferation was observed by the MTT assay. Transwell assays were carried out to observe the effect of miR-24 on cell migration. The effects of miR-24 on the expression of cyclin-dependent kinase (CDK) 4/6 and matrix metalloproteinase (MMP) 2 in A549 cells were examined by Western blotting. Results Survival analysis showed that patients with low miR-24 expression had a shorter survival time. The MTT-based viability assay revealed that overexpression of miR-24 inhibited A549 cell proliferation. Cell proliferation was promoted when miR-24 was inhibited. In the Transwell assay,overexpression of miR-24 significantly inhibited the A549 cell migration,and cell migration increased when miR-24 was inhibited. Western blotting analysis revealed that overexpression of miR-24 could inhibit CDK4/6 and MMP2 production in A549 cells. Inhibition of miR-24 was associated with increased production of CDK4/6 and MMP2 in A549 cells. Conclusion miR-24 can inhibit the proliferation and migration of A549 lung cancer cells.

Key words: miR-24, lung cancer, A549, proliferation, migration

中图分类号: 

  • R739.5
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