中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2019, Vol. 48 ›› Issue (2): 144-148.doi: 10.12007/j.issn.0258-4646.2019.02.012

• 论著 • 上一篇    下一篇

宫颈组织中Stat5b表达与宫颈病变的关系

吴思, 吴盈盈, 于淼, 王爽, 崔畅婉, 孙峥嵘   

  1. 中国医科大学附属盛京医院生物样本库, 沈阳 110004
  • 收稿日期:2018-06-11 出版日期:2019-02-28 发布日期:2019-01-05
  • 通讯作者: 孙峥嵘 E-mail:sunzr@sj-hospital.org
  • 作者简介:吴思(1990-),女,技师,本科.
  • 基金资助:
    辽宁省高等学校基本科研项目(LFWK201710)

Relationship between Stat5b Expression in Cervical Tissues and Lesions

WU Si, WU Yingying, YU Miao, WANG Shuang, CUI Changwan, SUN Zhengrong   

  1. Department of Biobank, Shengjing Hospital, China Medical University, Shenyang 110004, China
  • Received:2018-06-11 Online:2019-02-28 Published:2019-01-05

摘要: 目的 研究宫颈病变组织中信号转导和转录激活因子Stat5b及其磷酸化形式p-Stat5b的表达情况及其与不同程度宫颈病变之间的关系,探讨Stat5b在宫颈病变发生、发展中的作用。方法 以正常宫颈组织、低度鳞状上皮内病变(LSIL)、高度鳞状上皮内病变(HSIL)、宫颈癌组织共80例标本为研究对象,采用免疫组织化学染色评估Stat5b以及p-Stat5b的表达水平,分析其与宫颈病变程度的相关性。结果 Stat5b表达水平随宫颈病变的进展而升高,呈正相关。Stat5b在正常宫颈组织、LSIL、HSIL、宫颈癌中高表达比例分别为15.0%、0.0%、50.0%、80.0%,差异有统计学意义(P<0.05)。相反,p-Stat5b表达与宫颈病变的进展呈负相关,在正常宫颈组织、LSIL、HSIL、宫颈癌中高表达比例分别为80.0%、45.0%、5.0%、0.0%,差异有统计学意义(P<0.05)。结论 Stat5b在不同程度宫颈病变中的表达有差异,表明Stat5b信号分子可能与宫颈病变的发展有关。

关键词: Stat5b, 宫颈病变/宫颈癌, 免疫组织化学

Abstract: Objective To investigate the expression of signal transducer and activator of transcription 5b (Stat5b) and its phosphorylation (p-Stat5b) in cervical lesions,its relationship with different degrees of cervical lesions,and its role in the pathogenesis and development of cervical lesions. Methods We obtained 80 specimens,including normal cervical tissues,low-grade squamous intraepithelial lesions (LSIL),high-grade squamous intraepithelial lesions (HSIL),and cervical cancer tissues. To analyze the correlation between Stat5b and the degree of cervical lesions,immunohistochemical staining was performed to detect Stat5b and p-Stat5b expression. Results Increased Stat5b expression had a positive correlation with the development of cervical lesions. The percentages of Stat5b expressed in normal tissues,LSIL,HSIL,and cervical cancer tissues were 15.0%,0.0%,50.0%,and 80.0%,respectively,with significant differences among the groups (P<0.05). Conversely,the expression of p-Stat5b had a negative correlation with the development of cervical lesions. Expression of p-Stat5b in normal tissues,LSIL,HSIL,and cervical cancer tissues was 80.0%,45.0%,5.0%,and 0.0%,respectively,with statistically significant differences among the groups (P<0.05). Conclusion The expression of Stat5b is significantly different in different degrees of cervical lesions,suggesting that Stat5b signaling molecules may be involved in the development of cervical lesions.

Key words: Stat5b, cervical lesions/cervical cancer, immunohistochemistry

中图分类号: 

  • R737.33
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