中国医科大学学报

中国医科大学学报
  • 中文核心期刊
  • 中国科技核心期刊
  • 中国高校百佳科技期刊
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中国医科大学学报 ›› 2019, Vol. 48 ›› Issue (3): 210-215.doi: 10.12007/j.issn.0258-4646.2019.03.004

• 论著 • 上一篇    下一篇

Notch信号通路促进滑膜肉瘤细胞SW982增殖和侵袭的研究

高天1, 余铃2, 李舒1, 刘佳勇1, 白楚杰1, 薛瑞峰1, 张路1, 方志伟1, 樊征夫1   

  1. 1. 北京大学肿瘤医院暨北京市肿瘤防治研究所骨与软组织肿瘤科, 恶性肿瘤发病机制及转化研究教育部重点实验室, 北京 100142;
    2. 武汉大学人民医院骨1科, 武汉 430060
  • 收稿日期:2018-05-08 出版日期:2019-03-30 发布日期:2019-03-06
  • 通讯作者: 樊征夫 E-mail:zhengfufan@126.com
  • 作者简介:高天(1986-),男,主治医师,博士.
  • 基金资助:
    国家自然科学基金(81802689)

Notch Signaling Promotes Proliferation and Migration of SW982 Synovial Sarcoma Cells

Gao Tian1, Yu Ling2, Li Shu1, Liu Jiayong1, Bai Chujie1, Xue Ruifeng1, Zhang Lu1, Fang Zhiwei1, Fan Zhengfu1   

  1. 1. Department of bone and soft tissue tumor, Key laboratory of Carcinogenesis and Translational Research(Ministry of Education), Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing 100142, China;
    2. Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, China
  • Received:2018-05-08 Online:2019-03-30 Published:2019-03-06

摘要: 目的 探讨Notch信号通路对人滑膜肉瘤增殖及侵袭潜能的影响。方法 培养人滑膜肉瘤细胞SW982和人滑膜细胞,观察2种细胞中Notch信号通路相关蛋白的表达情况。利用NICD1过表达、CFB1干扰慢病毒及γ-分泌酶抑制剂DAPT调控SW982中Notch信号通路,通过CCK-8及划痕试验对Notch信号通路能否影响人滑膜肉瘤细胞SW982进行验证。结果 相比于正常滑膜细胞,Notch信号通路活性在人滑膜肉瘤中显著增高(P < 0.05)。上调Notch信号通路后SW982增殖及侵袭能力明显增高,而下调Notch信号通路会导致SW982增殖及侵袭能力明显降低(P < 0.05)。结论 抑制Notch信号通路可以显著降低人滑膜肉瘤的增殖和侵袭能力。

关键词: 滑膜肉瘤, Notch信号通路, 增殖, 侵袭

Abstract: Objective To investigate the effect of the Notch signaling pathway on the proliferation and invasion of human SW982 synovial sarcoma cells. Methods SW982 cells and normal human synovial cells were routinely cultured,and the expression of proteins related to the Notch pathway was compared. The Notch signaling pathway was manipulated by NICD1 overexpression,CFB1 shRNA lentivirus,and the γ-secretase inhibitor,DAPT. CCK-8 and wound healing assays were carried out to investigate the role of the Notch signaling pathway in SW982 cells. Results The Notch signaling pathway clearly showed higher activity in human SW982 synovial sarcoma cells than in normal human synovial cells (P < 0.05). The proliferation and invasion of SW982 cells were significantly upregulated by overexpressing NICD1; however,were suppressed by downregulating the Notch signaling pathway using CFB1 shRNA or DAPT (P < 0.05). Conclusion Our findings demonstrate that the proliferation and invasion of human SW982 synovial sarcoma cells are dependent on Notch signaling pathway activity.

Key words: synovial sarcoma, Notch signalling pathway, proliferation, invasion

中图分类号: 

  • R735.2
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