中国医科大学学报

中国医科大学学报
  • 中文核心期刊
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  • 中国高校百佳科技期刊
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中国医科大学学报 ›› 2019, Vol. 48 ›› Issue (3): 236-239.doi: 10.12007/j.issn.0258-4646.2019.03.009

• 论著 • 上一篇    下一篇

miR-362-3p在喉癌组织中的表达及其对喉癌Hep-2细胞迁移的影响

赵越, 孙媛媛, 佟雪, 岳鹏杰, 富伟能   

  1. 中国医科大学基础医学院遗传学教研室, 沈阳 110122
  • 收稿日期:2018-07-16 出版日期:2019-03-30 发布日期:2019-03-06
  • 通讯作者: 富伟能 E-mail:wnfu@cmu.edu.cn
  • 作者简介:赵越(1994-),女,硕士研究生.
  • 基金资助:
    辽宁省重点研发计划(2017225021);辽宁省高等学校基本科研项目(LQNK201726)

miR-362-3p Expression in Laryngeal Cancer Tissues and Its Effect on Migration of Hep-2 Cells

ZHAO Yue, SUN Yuanyuan, TONG Xue, YUE Pengjie, FU Weineng   

  1. Department of Genetics, College of Basic Medical Science, China Medical University, Shenyang 110122, China
  • Received:2018-07-16 Online:2019-03-30 Published:2019-03-06

摘要: 目的 探讨miR-362-3p在喉癌组织中的表达水平及其对喉癌Hep-2细胞迁移能力的影响。方法 收集50例喉癌组织及对应癌旁组织标本,实时PCR方法检测miR-362-3p在喉癌组织和癌旁组织中表达水平,分析miR-362-3p的表达与喉癌患者临床病理特征的关系;将miR-362-3p模拟物、抑制剂以及阴性对照miRNA分别转染喉癌Hep-2细胞,实时PCR检测转染效率;划痕和Transwell实验检测miR-362-3p对喉癌Hep-2细胞迁移能力的影响。结果 miR-362-3p在喉癌组织中表达水平显著高于癌旁组织(P < 0.05),且与喉癌患者淋巴结转移及临床分期有关;上调miR-362-3p表达水平能促进喉癌Hep-2细胞的迁移能力,下调miR-362-3p表达水平能抑制喉癌Hep-2细胞的迁移能力(均P < 0.05)。结论 miR-362-3p在喉癌组织中表达上调,且能够促进喉癌细胞的迁移能力,提示其发挥潜在癌基因作用。

关键词: miR-362-3p表达, 喉癌, 迁移

Abstract: Objective To explore miR-362-3p expression in laryngeal cancer tissues and its effect on migration of Hep2 cells. Methods miR-362-3p expression in 50 pairs of tumor and adjacent normal tissues was detected by real-time PCR after sample collection. The relationships between miR-362-3p expression and clinical pathological characteristics in patients with laryngeal cancer were analyzed. miR-362-3p mimic,inhibitor,and control microRNA were transfected into Hep-2 cells. Transfection efficiency was determined by real-time PCR. Wound-healing and transwell assays were used to evaluate Hep-2 migration. Results miR-362-3p expression was significantly higher in cancer tissues than in adjacent normal tissues (P < 0.05). miR-362-3p expression was statistically significantly related to node metastasis and clinical stage. Transfection with miR-362-3p mimic and inhibitor significantly increased and decreased Hep-2 cell migration,respectively (both P < 0.05). Conclusion miR-362-3p is up-regulated in laryngeal cancer tissues and promotes laryngeal cancer cell migration,suggesting that it acts as a potential oncogene in laryngeal cancer.

Key words: miR-362-3p expression, laryngeal cancer, migration

中图分类号: 

  • R739.6
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