中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2019, Vol. 48 ›› Issue (3): 255-259.doi: 10.12007/j.issn.0258-4646.2019.03.013

• 论著 • 上一篇    下一篇

EGCG促进AOM/DSS诱导小鼠结直肠腺瘤生成

刘刚, 周建平, 李新, 董明   

  1. 中国医科大学附属第一医院胃肠外科/疝与腹壁外科, 沈阳 110001
  • 收稿日期:2018-06-14 出版日期:2019-03-30 发布日期:2019-03-06
  • 通讯作者: 周建平 E-mail:zjphama@163.com
  • 作者简介:刘刚(1993-),男,硕士研究生.
  • 基金资助:
    辽宁省特聘教授基金(2012-512)

EGCG Promotes the Production of Colorectal Adenoma Induced by AOM/DSS in Mice

LIU Gang, ZHOU Jianping, LI Xin, DONG Ming   

  1. Department of Gastrointestinal Surgery/Hernia and Abdominal Wall Surgery, The First Hospital, China Medical University, Shenyang 110001, China
  • Received:2018-06-14 Online:2019-03-30 Published:2019-03-06

摘要: 目的 研究表没食子儿茶素没食子酸酯(EGCG)对氧化偶氮甲烷(AOM)/葡聚糖硫酸钠(DSS)诱导小鼠结直肠腺瘤的影响。方法 将30只雌性幼鼠随机分为对照组、AOM/DSS组和AOM/DSS+EGCG组,每组10只。饲养过程中每周观察各组小鼠体质量、精神状态以及排便情况,13周后处死小鼠,观察每组小鼠结直肠腺瘤发生率和肿瘤数量。采用免疫组化方法检测小鼠结直肠肿瘤组织中CD31及CD68的表达情况。结果 对照组小鼠无结直肠腺瘤发生,AOM/DSS组和AOM/DSS+EGCG组各有4只小鼠出现结直肠腺瘤,癌变率分别为50.00%(5/10)和66.67%(6/9)(P > 0.05)。在成瘤小鼠肿瘤数量上,AOM/DSS+EGCG组(8.25±2.06)高于AOM/DSS组(3.75±1.71)(P < 0.05)。AOM/DSS+EGCG组小鼠结直肠腺瘤组织中CD31和CD68的表达较AOM/DSS组高(P < 0.05)。结论 EGCG可能是结直肠非典型增生和结直肠腺瘤发生发展的一个促进因素。

关键词: 表没食子儿茶素没食子酸酯, 结直肠腺瘤, CD31, CD68

Abstract: Objective To study the effect of epigallocatechin gallate (EGCG) on colorectal adenoma induced by azoxymethane (AOM)/dextran sulfate sodium (DSS) in mice. Methods Thirty young,female mice were randomly divided into control,AOM/DSS,and AOM/DSS+EGCG groups (n=10 in each group). The body weight,mental status,and defecation of mice in each group was recorded every week,for 13 weeks,at the end of which the mice were sacrificed. The incidence of colorectal adenoma and the number of tumors in each group was recorded. Immunohistochemistry was used to detect the expressions of CD31 and CD68 in colorectal tumor tissues. Results No colorectal adenoma was found in mice of the control group. Colorectal adenoma was found in 4 mice of both AOM/DSS and AOM/DSS+EGCG groups. the incidence of colorectal adenoma was 50.00% (5/10) in the AOM/DSS group and 66.67% (6/9) in the AOM/DSS+EGCG group (P > 0.05). The number of tumors in the AOM/DSS+EGCG group was higher than that in the AOM/DSS group (8.25±2.06 vs 3.75±1.71,P < 0.05). The expression levels of CD31 and CD68 in the AOM/DSS+EGCG group were higher than those in the AOM/DSS group (P < 0.05). Conclusion EGCG might promote the occurrence of colon dysplasia and colon adenoma.

Key words: epigallocatechin gallate, colorectal adenoma, CD31, CD68

中图分类号: 

  • R574.62
[1] BAHRAMI A,KHAZAEI M,HASANZADEH M,et al. Therapeutic potential of targeting PI3K/AKT pathway in treatment of colorectal cancer:rational and progress[J]. J Cell Biochem,2018,119(3):2460-2469. DOI:10.1002/jcb.25950.
[2] ABRAHAM C. Inflammatory bowel disease[J]. N Engl J Med,2009,361(21):2066-2078. DOI:10.1056/NEJMra0804647.
[3] FEAGINS LA,SOUZA RF. Carcinogenesis in IBD:potential targets for the prevention of colorectal cancer[J]. Nat Rev Gastroenterol Hepatol,2009,6(5):297-305. DOI:10.1038/nrgastro.2009.44.
[4] HARATIFAR S,MECKLING KA,CORREDIG M. Antiproliferative activity of tea catechins associated with casein micelles,using HT29 colon cancer cells[J]. J Dairy Sci,2014,97(2):672-678. DOI:10.3168/jds.2013-7263.
[5] WUBETU GY,SHIMADA M,MORINE Y,et al. Epigallocatechin gallate hinders human hepatoma and colon cancer sphere formation[J]. J Gastroenterol Hepatol,2016,31(1):256-264. DOI:10.1111/jgh.13069.
[6] GUAN F,LIU AB,LI G,et al. Deleterious effects of high concentrations of (-) -epigallocatechin-3-gallate and atorvastatin in mice with colon inflammation[J]. Nutr Cancer,2012,64(6):847-855. DOI:10.1080/01635581.2012.695424.
[7] 刘维新,张绅,任益,等. 溃疡性结肠炎及溃疡性结肠炎相关性结直肠癌小鼠模型中血管生成因子的表达及其与血管新生的关系[J].中国医科大学学报,2013,42(3):231-234.
[8] JU J,KIM YJ,PARK ES. Korean solar salt ameliorates colon carcinogenesis in an AOM/DSS-induced C57BL/6 mouse model[J]. Prev Nutr Food sci,2017,22(2):149-155. DOI:10.3746/pnf.2017.22.2.149.
[9] WEIDNER N. Current pathologic methods for measuring intratumoral microvessel density within breast carcinoma and other solid tumors[J]. Breast Cancer Res Treat,1995,36(2):169-180.
[10] AXELRAD JE,LICHTIGER S. Inflammatory bowel disease and cancer:the role of inflammation,immunosuppression,and cancer treatment[J]. World J Gastroenterol,2016,22(20):4794-4801. DOI:10.3748/wjg.v22.i20.4794.
[11] ROSSIN D,CALFAPIETRA S,SOTTERO B,et al. HNE and cholesterol oxidation products in colorectal inflammation and carcinogenesis[J]. Free Radic Biol Med,2017,111:186-195. DOI:10.1016/j.freeradbiomed.2017.01.017.
[12] LI Y,DE HAAR C,CHEN M,et al. Disease-related expression of the IL6/STAT3/SOCS3 signaling pathway in ulcerative colitis and ulcerative colitis-related carcinogenesis[J]. Gut,2010,59(2):227-235. DOI:10.1136/gut.2009.184176.
[13] TANAKA T,KOHNO H,SUZUKI R,et al. A novel inflammation-related mouse colon carcinogenesis model induced by azoxymethane and dextran sodium sulfate[J]. Cancer Sci,2003,94(11):965-973. DOI:10.1111/j.1349-7006.2003.tb01386.x
[14] SENO H,OSHIMA M,ISHIKAWA TO,et al. Cyclooxygenase 2-and prostaglandin E (2) receptor EP (2) -dependent angiogenesis in Apc (Delta716) mouse intestinal polyps[J]. Cancer Res,2002,62(2):506-511.
[15] SAAD RS,EL-GOHARY Y,MEMARI E,et al. Endoglin (CD105) and vascular endothelial growth factor as prognostic markers in esophageal adenocarcinoma[J]. Hum Pathol,2005,36(9):955-961. DOI:10.1016/j.humpath.2005.06.019.
[16] DELIU IC,NEAGOE CD,BEZNĂ M,et al. et al. Correlations between endothelial cell markers CD31,CD34 and CD105 in colorectal carcinoma.[J]. Rom J Morphol Embryol,2016,57(3):1025-1030.
[17] MOHAMED SY,MOHAMMED HL,IBRAHIM HM,et al. Role of VEGF,CD105,and CD31 in the prognosis of colorectal cancer cases[J]. J Gastrointest Cancer,2017. DOI:10.1007/s12029-017-0014-y.
[18] MANTOVANI A,SCHIOPPA T,PORTA C,et al. Role of tumor-associated macrophages in tumor progression and invasion[J]. Cancer Metastasis Rev,2006,25(3):315-322. DOI:10.1007/s10555-006-9001-7.
[19] ZHANG Y,SIME W,JUHAS M,et al. Crosstalk between colon cancer cells and macrophages via inflammatory mediators and CD47 promotes tumor cell migration[J]. Eur J Cancer,2013,49(15):3320-3334. DOI:10.1016/j.ejca.2013.06.005.
[20] WANG W,LI X,ZHENG D,et al. Dynamic changes and functions of macrophages and M1/M2 subpopulations during ulcerative colitis-associated carcinogenesis in an AOM/DSS mouse model[J]. Mol Med Rep,2015,11(4):2397-2406. DOI:10.3892/mmr.2014.3018.
[21] ZHOU Q,XIAN M,XIANG S,et al. All-trans retinoic acid prevents osteosarcoma metastasis by inhibiting M2 polarization of tumor-associated macrophages[J]. Cancer Immunol Res,2017,5(7):547-559. DOI:10.1158/2326-6066.CIR-16-0259.
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