中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2009, Vol. 38 ›› Issue (10): 727–.

• 基础医学 • 上一篇    下一篇

锌对大鼠生长板软骨细胞ZnT-7表达的影响

初立伟; 杨茂伟; 于江; 王铜浩; 李亚伦   

  1. 中国医科大学附属第一医院骨科
  • 出版日期:2009-10-20 发布日期:2010-04-02
  • 通讯作者: 杨茂伟 E-mail:mwyang@mail.cmu.edu.cn

Effects of Zinc on the Expression of ZnT-7 in Growth Plate Chondrocytes

  • Online:2009-10-20 Published:2010-04-02

摘要:

目的研究锌对大鼠生长板软骨细胞锌转移体-7(ZnT-7)表达的影响及其规律。方法原代培养Wistar大鼠肋生长板软骨细胞,不同浓度的锌螯合剂TPEN分别处理培养生长板软骨细胞12h。免疫组化检测生长板软骨细胞特异性Ⅱ型胶原表达。免疫荧光技术对ZnT-7进行定位研究,实时RT-PCR和Western
      blot检测ZnT-7表达。结果Ⅱ型胶原免疫组化检测阳性。ZnT-7在生长板软骨细胞中定位于高尔基体上;Western
      blot和RT-PCR结果显示;5μmol/L(TPEN)组与对照组相比略有增高,而10μmol/L、20μmol/L组与对照组相比逐渐降低。结论ZnT-7定位于高尔基体上,同时在缺锌的条件下其对锌离子稳定有一定的代偿作用。

关键词: 锌, 生长板软骨细胞, 锌转移体

Abstract:

Objective To study the effects of zinc on the expression of zinc
      transporter-7(ZnT-7)in the proliferation of the rat growth plate
      chondrocytes. Methods Growth plate chondrocytes were isolated from rib
      cartilage of Wistar rat. The cells were treated with zinc chelating agent
      N,N,N',N'-tetrakis (2-pyridylmethyl)ethylene-diamine (TPEN)of different
      concentration(0,5,10 and 20 μmol/L)for 12 hours. The expression of rat
      growth plate chondrocytes specificity collagen type Ⅱ was detected by
      immunohistochemistry. The localization of ZnT-7 was checked by
      immunofluorescent staining. Real-time RT-PCR and Western blot were used to
      measure the expression level of ZNT-7 in the cell. Results The result of
      the immunofluorescence showed that ZnT-7 located in the Golgi apparatus.
      The expression level of ZnT-7 was slightly higher in the cells treated
      with 5 μmol/L TPEN than the control group,while it was lower in the cells
      treated with 10 or 20 μmol/L TPEN than the control group. Conclusion ZnT-7
      locates in Golgi apparatus and maintains the zinc ion stabilization
      in the condition of the zinc depletion. 

Key words: zinc, growth plate chondrocytes, zinc transporter

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