中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2009, Vol. 38 ›› Issue (10): 737–.

• 基础医学 • 上一篇    下一篇

HA14-1对环磷酰胺治疗小鼠Lewis肺癌增敏作用的实验研究

谭明旗; 边明艳   

  1. 中国医科大学附属盛京医院第二呼吸内科; 辽宁省金秋医院内科
  • 出版日期:2009-10-20 发布日期:2010-04-02
  • 通讯作者: 谭明旗 E-mail:mingqi.tan@yahoo.com.cn

HA14-1 Sensitizes Chemotherapy of Murine Lewis Lung Carcinoma to Cyclophosphamide

  • Online:2009-10-20 Published:2010-04-02

摘要:

目的观察HA14-1对环磷酰胺(CTX)治疗Lewis肺癌小鼠的化疗增效作用,探讨其可能的作用机制。方法建立Lewis肺癌小鼠肿瘤模型,将40只C57BL/6小鼠随机分为4组:生理盐水组、CTX组、HA14-1组和CTX+HA14-1组,给药7d。于肿瘤接种22d处死小鼠,描绘各组肿瘤体积增长曲线、计算抑瘤率,免疫组织化学方法测定治疗前后Lewis肺癌细胞Bcl-2、Bax和Caspase-9蛋白的表达情况。结果HA14-1组与生理盐水组比较无明显抑制肿瘤体积的作用,CTX组、HA14-1组和CTX+HA14-1组的肿瘤体积较生理盐水组增长慢。HA14-1组的抑瘤率与生理盐水组比较无明显增加(P>0.05);CTX+HA14-1组及CTX组与生理盐水组比较,CTX+HA14-1组与CTX组比较,抑瘤率均明显增加(P<0.05)。CTX组、HA14-1组和CTX+HA14-1组Bcl-2蛋白表达均减少,且CTX+HA14-1组较CTX组Bcl-2蛋白明显减少(P<0.05)。CTX组、HA14-1组和CTX+HA14-1组Bax、Caspase-9蛋白表达增加,与生理盐...更多水组比较差异有统计学意义(P<0.05);CTX+HA14-1组比CTX组Bax、Caspase-9蛋白表达明显增加(P<0.05)。结论HA14–1可以增加CTX的化疗作用,其作用机制可能是通过抑制Bcl-2蛋白的表达,增加Bax、Caspase-9蛋白表达,从而促使肿瘤细胞凋亡。

关键词: HA14-1, Lewis肺癌, 环磷酰胺, 增敏, HA14-1, Lewis肺癌, 环磷酰胺, 增敏

Abstract:

Objective To observe HA14-1 sensitizes Lewis lung carcinoma in mice
      to cyclophosphamide(CTX)and to explore its possible mechanism. Methods
      Forty Lewis lung carcinoma model mice were randomly divided into 4
      groups:normal saline group,CTX group,HA14-1 group,CTX+HA14-1 group. After
      the treatment of 7 days,all of the mice were killed on the 22nd day of
      tumor inoculation. The tumor volume growth curve of each group was
      described;tumor inhibition rate was caculatued;Bcl-2,Bax,Caspase-9 protein
      expression levels before and after the treatment were determined by
      immunohistochemistry. Results Compared to the normal saline group,HA14-1
      group had no significant effect on inhibiting tumor volume,and the tumor
      volume in HA14-1 group increased less slowly than that of CTX group,HA14-1
      group and CTX+HA14-1 group. Compared to the normal saline group,the tumor
      inhibition rate of HA14-1 group had no significant in-crease(P >
      0.05),while that of CTX group and CTX + HA14-1 group increased
      significantly(P < 0.05);compared to the CTX group,the tumor
      inhibition rate of CTX + HA14-1 group increased significantly (P < 0.05).
      Bcl-2 protein expression levels in CTX group,HA14-1 group and CTX+HA14-1
      group were lower than that in the normal saline group;compared to CTX
      group,Bcl-2 protein expression of CTX + HA14-1 group reduced
      significantly. Compared to the normal saline group,the expression levels
      of Bax and Caspase-9 protein in CTX group,HA14-1 group and CTX+HA14-1
      group increased significantly (P < 0.05);compared to CTX group,CTX +
      HA14-1 group increased more significantly (P < 0.05). Conclusion HA14-1
      might enhance the efficiency of CTX chemotherapy via inhibiting the
      expression of Bcl-2,increasing the expression of Bax and caspase-9 and
      promoting tumor cell apoptosis.

Key words: HA14-1, Lewis lung carcinoma, cyclophamide, sensitization, HA14-1, Lewis lung carcinoma, Cyclophamide, sensitization

[1] 何林秀,孙明立,边竞,于兆进,陈文捷,李亚楠,魏敏杰. ω-3鱼油脂肪乳对环磷酰胺所致小鼠胃黏膜损伤的保护作用[J]. 中国医科大学学报, 2015, 44(12): 1090-1093.
[2] 吴春丽,蔡峰,李光,陈延治. FUDR对鼻咽癌CNE-1细胞系的放射增敏作用[J]. 中国医科大学学报, 2012, 41(11): 1022-1025.
[3] 谭明旗, 边明艳. HA14-1诱导小鼠Lewis肺癌细胞凋亡的实验研究[J]. 中国医科大学学报, 2009, 38(9): 668-.
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