中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2009, Vol. 38 ›› Issue (10): 755–.

• 口腔医学 • 上一篇    下一篇

小鼠钟状期牙胚中Frizzled蛋白的表达

高洁; 叶新萍; 高妍; 刘飞   

  1. 中国医科大学附属口腔医院儿童牙科; 辽河油田第二医院口腔科
  • 出版日期:2009-10-20 发布日期:2010-04-02
  • 通讯作者: 高洁 E-mail:jiekao117@hotmail.com

Expression of Frizzled Protein in Mouse Tooth Germ at Bell Stage

  • Online:2009-10-20 Published:2010-04-02

摘要:

目的观察牙胚发育钟状期时Frizzled蛋白的表达情况,探讨Wnt/Frizzled信号分子的作用及机制。方法取胚胎17d和19d的胎鼠,另取生后2d的幼鼠,分别制作下颌第1磨牙的切片,运用免疫荧光技术显示Frizzled蛋白的阳性分布部位,并在荧光显微镜下观察。结果胚胎17d时Frizzled蛋白在成釉器的内、外釉上皮有弱阳性分布,胚胎19d时在前成釉细胞和前成牙本质细胞的顶端有Frizzled蛋白的阳性表达,生后2d的标本上成釉细胞和成牙本质细胞的顶端Frizzled蛋白呈强阳性表达。结论Wnt/Frizzled信号分子参与了成釉细胞和成牙本质细胞的分化过程的调节。

关键词: 牙胚, 钟状期, Frizzled蛋白, 成釉细胞, 成牙本质细胞, 牙胚, 钟状期, Frizzled蛋白, 成釉细胞, 成牙本质细胞

Abstract:

Objective To observe the expression of Frizzled protein in mouse
      tooth germ at bell stage and explore the possible function of Wnt/Frizzled
      signal molecules. Methods Mouse embryos at 17 and 19 days of gestation
      (E17 and E19)as well as mice postnatal day 2 (PN2)were employed in our
      study. The frozen sections of the first lower molar were made and indirect
      immunofluorescence technique was carried out on the sections. The location
      of Frizzled protein was observed under the fluorescence microscope.
      Results Frizzled protein was expressed weakly in the inner-and
      outer-dental epithelium at E17. At E19,the positive staining was found to
      distribute at the summit of both pre-ameloblasts and pre-odontoblasts. At
      P2,Frizzled protein was expressed strongly at the summit of ameloblasts
      and odontoblasts. Conclusion Wnt/Frizzled signal molecules may be involved
      in the differentiation of ameloblasts and odontoblasts.

Key words: tooth germ, bell stage, Frizzled protein, ameloblast, odontoblast

[1] 陈井阳 ,钟鸣 ,沈文静 ,刘洁. miR-608 在成釉细胞瘤中的表达及意义[J]. 中国医科大学学报, 2017, 46(8): 724-728.
[2] 李波 , 高洁 , 刘冬娟. 年轻恒牙组织形态的超微结构观察 [J]. 中国医科大学学报, 2012, 41(2): 135-138.
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