中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2009, Vol. 38 ›› Issue (11): 810–.

• 基础医学 • 上一篇    下一篇

p16~(INK4a)基因在CLD新生鼠肺成纤维细胞中的动态表达

赵诗萌; 胡瑜; 薛辛东; 胡畔   

  1. 中国医科大学附属第一医院新生儿科; 中国医科大学附属盛京医院儿科; 中国医科大学第91期临床医学系
  • 出版日期:2009-12-20 发布日期:2010-04-14
  • 通讯作者: 薛辛东 E-mail:xdxue@163.com

Dynamic Expression of p16INK4a Gene in Lung Fibroblasts of Neonatal Rats with Chronic Lung Disease

  • Online:2009-12-20 Published:2010-04-14

摘要:

目的探讨p16INK4a基因在高氧致慢性肺疾病(CLD)新生鼠肺成纤维细胞中的动态表达。方法足月新生SD大鼠于生后12h内分别持续吸入0.85高氧和空气,于3,7,14,21d随机处死动物后,进行肺成纤维细胞的原代培养,应用流式细胞仪测细胞周期;免疫组化、Western
      blot法检测p16INK4a蛋白表达;real-time PCR法检测p16INK4a
      mRNA含量变化。结果生后3d时2组p16INK4a蛋白及mRNA的表达无明显差异(P>0.05),生后7d时高氧组表达开始减少(P<0.05),14d、21d明显减少(P<0.01)。结论高氧可下调肺成纤维细胞p16INK4a基因的表达,使得其抑制细胞增殖的作用减弱,肺成纤维细胞过度增殖,最终导致肺纤维化的发生。

关键词: 高氧, 早产儿慢性肺疾病, p16INK4a基因, 肺成纤维细胞

Abstract:

Objective To explore the dynamic expression of p16INK4a gene in
      lung fibroblasts of neonatal rats with chronic lung disease(CLD).Methods
      Full-term newborn SD rats were continuously exposed to oxygen(0.85)or room
      air within 12 hours after birth.The rat pups were killed on postnatal days
      3,7,14,and 21.The primary culture of lung fibroblasts were performed,and
      the cell cycle was detected by flow cytometry.The expressions of p16INK4a
      protein and mRNA in lung fibroblasts were determined by
      immunohistochemistry and Western-blot and by real-time
      PCR,respectively.Results No significant difference in the expressions of
      p16INK4a protein and mRNA was found between hyperoxia and control groups
      on postnatal day 3.Compared with control group,the expression of pI6INK4a
      in hyperoxia group began to decrease since postnatal day 7(P<0.05)and
      significantly decreased on postnatal days 14 and 21(P<0.01).Conclusion
      Hyperoxia can down-regulate the expression of p16INK4a gene and induce
      excessive proliferation in lung fibroblasts and ultimately results in
      pulmonary interstitial fibrosis.

Key words: hyperoxia, chronic lung disease, p16INK4a gene, lung fibroblast

[1] 王思,曹旭,刘冬妍. 新生大鼠高氧动物模型肠道AKT的变化[J]. 中国医科大学学报, 2013, 42(7): 577-581.
[2] 刘冬妍,王思,姜腾,曹旭, 周潇男 . 高氧致新生大鼠肠屏障功能的变化[J]. 中国医科大学学报, 2012, 41(8): 679-681.
[3] 党红星 ,杨林 ,王少华 ,刘聪 ,方芳,许峰 . 高氧暴露下新生大鼠肺组织Smo和Gli1蛋白的表达和意义 [J]. 中国医科大学学报, 2012, 41(7): 625-629.
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