中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2009, Vol. 38 ›› Issue (11): 849–.

• 临床医学 • 上一篇    下一篇

LOX-1在D-葡萄糖培育人肾小球系膜细胞的表达

李萍; 梁琳琅; 杨威; 向军; 张颖; 杨昕   

  1. 中国人民解放军沈阳军区总医院内分泌科; 中国人民解放军沈阳军区总医院泌尿外科
  • 出版日期:2009-12-20 发布日期:2010-04-14
  • 通讯作者: 梁琳琅 E-mail:Lianglllj@sina.com

Expression of Lectin-like Oxidized LDL Receptor-1 in Human Glomerular Mesangial Cells Incubated with D-glucose

  • Online:2009-12-20 Published:2010-04-14

摘要:

目的研究血凝素样氧化低密度脂蛋白受体1(LOX-1)在D-葡萄糖培养人肾小球系膜细胞(HGMC)的表达,探讨糖尿病肾脏损伤的机制。方法体外培养HGMC,用RT-PCR法检测LOX-1
      mRNA的表达,用Western blot法检测p38MAPK蛋白质的相对含量。结果D-葡萄糖以时间和浓度依赖的方式增加LOX-1
      mRNA的表达,该作用可被LOX-1特异性阻滞剂JTX92部分抑制;D-葡萄糖以时间和浓度依赖的方式上调p38MAPK蛋白的表达,该作用也可被LOX-1特异性阻滞剂JTX92部分抑制。结论D-葡萄糖能以时间和浓度依赖的方式增加LOX-1的表达。高浓度D-葡萄糖可能通过上调LOX-1
      mRNA表达,激活细胞内的p38MAPK信号传递途径,参与糖尿病肾病的发生发展。

关键词: 血凝素样氧化低密度脂蛋白受体1, p38丝裂原活化蛋白激酶, 人肾小球系膜细胞, 糖尿病肾病, 血凝素样氧化低密度脂蛋白受体1, p38丝裂原活化蛋白激酶, 人肾小球系膜细胞, 糖尿病肾病

Abstract:

Objective To detect the expression of lectin-like oxidized LDL
      receptor-1(LOX-1) in human glomerular mesangial cells(HGMC)incubated with
      D-Glucose,and to explore the mechanism of diabetic nephropathy.Methods
      HGMC were cultured in vitro.The expression of LOX-1 mRNA was determined by
      reverse transcription polymerase chain reaction.The activity of p38
      mitogen activated protein kinase(MAPK)was measured by Western blot.Results
      D-Glucose increased the expression of LOX-1 mRNA and p38 MAPK protein in a
      concentration-and time-dependent manner,which was partially inhibited by
      JTX92.Conclusion D-Glucose may be involved in the occurrence and
      development of diabetic nephropathy by increasing the expression of LOX-1
      mRNA and activating p38 MAPK signaling pathway.

Key words: lectin-like oxidized LDL receptor-1, p38 mitogen activated protein kinase, human glomerular mesangial cell, diabetic nephropathy, Lectin-like oxidized low-density lipoprotein receptor 1, p38 mitogen activated protein kinase, Human glomerular mesangial cell, diabetic nephropathy

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