中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2009, Vol. 38 ›› Issue (11): 858–.

• 临床医学 • 上一篇    下一篇

TGF-β_1与CTL在肾癌围手术期的相关性研究

刘剑平; 王禾; 秦卫军; 王福利; 李育新   

  1. 第四军医大学西京医院泌尿外科
  • 出版日期:2009-12-20 发布日期:2010-04-14
  • 通讯作者: 王禾 E-mail:xjyymnwk@fmmu.edu.cn

Relationship between TGF-β_1 and CTL during Perioperative Period in Patients with Renal Carcinoma

  • Online:2009-12-20 Published:2010-04-14

摘要:

目的探讨转化生长因子β(1TGF-β1)与细胞毒性T淋巴细胞(CTL)杀伤活性、增殖能力在肾癌围手术期的相关性。方法采用免疫磁珠分离法分离纯化15例正常人和45例肾癌患者外周血CTL,流式细胞仪分析细胞表型,MTT法检测CTL杀伤活性,3H-TdR掺入法检测CTL增殖能力,酶联免疫吸附试验方法检测血清TGF-β1浓度。结果肾癌根治切除术前患者血清TGF-β1浓度明显高于正常对照组(P<0.01),根治术后血清TGF-β1浓度明显下降(P<0.01)。肾癌根治术前患者CTL杀伤活性、增殖能力明显低于正常对照组(P<0.01),根治术后CTL杀伤活性、增殖能力明显增加(P<0.01),在肾癌围手术期TGF-β1水平与CTL杀伤活性、增殖能力呈负相关(r分别为0.99、0.954,P<0.01)。结论肾癌细胞分泌大量TGF-β1,能明显抑制外周血CTL的杀伤活性和增殖能力。

关键词: 转化生长因子β1, 细胞毒性T淋巴细胞, 肾癌, 根治性肾切除术

Abstract:

Objective To investigate the relationship between transforming
      growth factor-βl(TGF-β)land activities of cytotoxic T
      lymphocyte(CTL)during perioperative period in patients with renal
      carcinoma.Methods CTLs were islolated by magnetic activated cell sorting
      from peripheral blood of 15 healthy adults and 45 patients with renal
      carcinoma.The phenotype was analyzed by flow cytometry.The killing and
      proliferative activities of CTL were determined by 51Cr-release assay and
      3H-TdR incorporative method,respectively.The serum level of TGF-βl was
      measured with enzyme-linked immunosorbent assay.Results Compared with
      healthy controls,the serum level of TGF-βl in patients with renal
      carcinoma was significantly higher before radical nephrectomy and
      significantly lower after the operation(both P < 0.01).The killing and
      proliferative activities of CTL in patients with renal carcinoma were
      significantly lower than those in healthy control before the
      operation(P<0.01),and the activities significantly increased after
      the operation(P<0.01).TGF-βl level was negatively correlated with the
      killing and proliferative activities of CTL(r=0.99 and r=0.954,both
      P<0.01).Conclusion Renal carcinoma cells could secrete large amounts of
      TGF-βl,which can obviously inhibit the killing and proliferative
      activities of CTL in peripheral blood.

Key words: transforming growth factor-βl, cytotoxic T lymphocyte, renal carcinoma, radical nephrectomy

[1] 孟凡东,李妍,吴迪,蒋涛,王扬,隋承光,姜又红. Wnt/β-catenin 信号通路中 Wnt 基因对肾癌细胞的生物学影响[J]. 中国医科大学学报, 2016, 45(4): 289-292.
[2] 刘佳,虞巍,杜鹏,何群,金杰,周利群,韩文科,杨勇. 肾癌术后肾上腺孤立转移灶的外科治疗方式比较:18例经验报告[J]. 中国医科大学学报, 2015, 44(12): 1125-1128.
[3] 李青楠,张恒,董喆,李军. 肾癌合并静脉癌栓的生存分析[J]. 中国医科大学学报, 2014, 43(9): 846-849.
[4] 徐之鹏,李青楠,董喆,张恒,李军. 青年肾癌的临床特点分析[J]. 中国医科大学学报, 2013, 42(9): 852-853.
[5] 荆宏伟,孔垂泽,梁凯,刘涛. 后肾腺瘤11例诊治分析[J]. 中国医科大学学报, 2013, 42(8): 760-761.
[6] 原劲杨,刘苏来,俞秋霞,王毅,王涌泉,安瑞华. 肾癌阴茎转移致阴茎异常勃起1例报告并文献复习[J]. 中国医科大学学报, 2013, 42(1): 91-92.
[7] 黄业翔,孔垂泽,刘涛,姜元军. 单通道无气腹后腹腔镜肾癌根治性切除术临床应用体会[J]. 中国医科大学学报, 2011, 40(9): 848-.
[8] 王新利, 李卿, 高婷, 付立叶, 王扬, 蒋涛, 姜又红. 树突状细胞分泌的exosomes体外诱导特异性CTL抗肾癌的初步研究[J]. 中国医科大学学报, 2010, 39(1): 4-.
[9] 陈硕, 马健飞, 杨丽娜, 樊怡. 血必净对高糖环境下大鼠腹膜间皮细胞分泌晚期炎性因子HMGB-1和TGF-β_1的影响[J]. 中国医科大学学报, 2009, 38(12): 895-.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!

中国医科大学学报版权所有©2018

未经允许,严禁擅自转载本站图文资料

地址:中国 沈阳市沈北新区蒲河路77号 110122

辽ICP备05014850

JOURNAL OF CHINA MEDICAL UNIVERSITY

ADDRESS: NO.77 PUHE ROAD

SHENYANG NORTH NEW AREA, SHENYANG

LIAONING PROVINCE, P.R. CHINA