中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2009, Vol. 38 ›› Issue (12): 888–.

• 基础医学 • 上一篇    下一篇

重组人促红细胞生成素对心肺复苏后大鼠脑氧合功能的影响

林生; 于铁英; 孟凌新   

  1. 烟台市烟台山医院麻醉科; 中国医科大学附属盛京医院麻醉科
  • 出版日期:2010-01-20 发布日期:2010-04-19
  • 通讯作者: 孟凌新 E-mail:meng_lingxin@hotmail.com

Cerebral Oxygenation and Ultrastructure after Cardiopulmonary Resuscitation

  • Online:2010-01-20 Published:2010-04-19

摘要:

目的探讨重组人促红细胞生成素(rhEPO)对心肺复苏后脑损伤的保护作用。方法雄性大鼠75只,随机分为3组,对照组(C组),肾上腺素组(AD组),肾上腺素+rhEPO组(E组),每组25只。麻醉后气管切开行机械通气。夹闭气管导管法建立大鼠心跳呼吸停止模型。E组大鼠在窒息前24h、16h腹腔注射rhEPO3000U/kg,窒息前10min静脉注入rhEPO30U/kg。AD组和E组呼吸心跳停止7min后恢复通气,经主动脉注入复苏合剂进行复苏。C组不予以窒息、复苏。C组动脉置管15min后,AD组、E组在自主循环恢复后1h、2h、3h采集脑组织标本及动、静脉血,进行血气分析。结果两组初期复苏率无显著差异(P>0.05)。与AD组比较,E组各时点颈动-静脉氧含量差(Ca-jO2)及脑氧摄取率(CEO2)明显升高(P<0.05)。同时电镜结果显示,E组超微结构改变明显减轻。结论促红细胞生成素虽不能提高初期复苏率,但能够减轻继发性脑损伤,增加脑氧摄取率,改善脑氧合功能。

关键词: 心肺复苏, 脑氧摄取率, 促红细胞生成素

Abstract:

Objective To evaluate the effects of recombinant human
      erythropoietion (rhEPO)given before cardiopulmonary resuscitation (CPR)on
      postresuscitation cerebral injury. Methods Seventy-five male Wistar rats
      (200300 g)were randomly divided into 3 groups: group I control(C,n
      =25);groupⅡepinephrine(AD,n =25)and group Ⅲepinephrine+erythropoietin(E,n
      =25). After anesthesia,all animals were taken mechanical ventilation by
      incision of trachea and made model of cardiorespiratory arrest. Before
      asphyxia for 24 and 16 hours,erythropoietin(EPO 3 000 U/Kg)was injected
      into abdominal cavity,and EPO(30 U/kg)was injected into vein at 10 min
      before asphyxia. CPR was started after 7 min of cardiorespiratory arrest
      and epinephine 100 μg/kg was given via carotid artery. Group C was
      subjected to no asphyxia or resuscitation .Arterial and jugular venous
      blood and brain tissue sample were collected on the point 1 h,2 h,and 3 h
      after ROSC in group AD and E. The corresponding data from C group was
      assigned to 15 min after surgical preparation. Results There is not
      obvious different basic resuscitation rate in the two groups. CEO2and
      C(a-jv)O2were significantly increased as compared to the value in group AD
      (P < 0.05). Electron microscopy showed that ultrastructural changes in
      Group E significantly were reduced. Conclusion Although rhEPO can not
      improve the basic resuscitation rate,it can relieve the injury of cerebral
      ultrastructure,increase cerebral extraction rate of oxygen,and improve
      cerebral oxygenation in post-resuscitation rats.

Key words: cardiopulmonary resuscitation, cerebral extraction of oxygen; erythropoietin

[1] 王娜娜,王文生,刘鑫璐. 促红细胞生成素对缺血再灌注心肌HSP70及ERK1/2表达的影响[J]. 中国医科大学学报, 2012, 41(9): 788-790.
[2] 王勇,铁欣昕,姚长义,王运杰. 重组人促红细胞生成素对脑缺血再灌注小鼠海马TNF-α表达的影响[J]. 中国医科大学学报, 2012, 41(5): 396-398.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!

中国医科大学学报版权所有©2018

未经允许,严禁擅自转载本站图文资料

地址:中国 沈阳市沈北新区蒲河路77号 110122

辽ICP备05014850

JOURNAL OF CHINA MEDICAL UNIVERSITY

ADDRESS: NO.77 PUHE ROAD

SHENYANG NORTH NEW AREA, SHENYANG

LIAONING PROVINCE, P.R. CHINA