中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2009, Vol. 38 ›› Issue (9): 666–.

• 基础医学 • 上一篇    下一篇

奥美沙坦对自发性高血压大鼠延髓腹外侧区AT1受体mRNA表达的影响

 张占帅, 王绍久, 王一丁, 林英子, 朱杰     

  1. 中国医科大学附属第四医院急诊科
  • 出版日期:2009-09-20 发布日期:2010-03-30
  • 通讯作者: 朱杰 E-mail:cmu4h zhujie@126.com

Effect of Olmesartan on the Expression of AT1 Receptor in Rostral Ventrolater Medulla of the Spontaneously Hypertensive Rat

  • Online:2009-09-20 Published:2010-03-30

摘要:

目的探求奥美沙坦(olmesartan)对自发性高血压大鼠(SHR)延髓腹外侧头端区(RVLM)血管紧张素Ⅱ1型(AT1)受体mRNA表达的影响。方法实验大鼠分作SHR对照组(正常饮水)、SHR-olme组[奥美沙坦,30mg(/kg·d)]和WKY组(正常饮水)。4周后,断头取材,采用RT-PCR方法检测RVLM内AT1受体mRNA的水平,并进行光密度测定。结果给药1周后奥美沙坦组血压均能够达标。SHR-olme组RVLM内AT1受体mRNA平均光密度(MOD)明显低于SHR组[(0.94±0.41)vs(2.41±0.37),P<0.01],但高于WKY组(0.81±0.22,P<0.05)。结论奥美沙坦能够显著减少SHR的RVLM组织中AT1受体mRNA表达。

关键词: 延髓腹外侧头端, 自发性高血压大鼠, 血管紧张素Ⅱ1型受体, 奥美沙坦

Abstract:

Objective To study the effect of olmesartan on the expression of
      AT1 receptor mRNA in rostral ventrolater medulla(RVLM)of the spontaneously
      hypertensive rat (SHR). Methods Rats were divided into SHR contral group
      (normal drinking),SHR-Olme group [(olmesartan,30 mg(/kg·d)],and
      Wistar-Kyoto rats(WKY)group (normal drinking). Four weeks later,these rats
      were decapitated to death and got the brain tissue. AT1 receptor mRNA
      level in RVLM was measured by RT-PCR and analyzed with optic density.
      Results The blood pressure of all SHR-olme rats reached normal level after
      administration of olmesartan for a week. The mean optic density(MOD) of
      AT1 receptor mRNA in RVLM of SHR-olme was much reduced compared with SHR
      group[(0.94±0.41)vs(2.41±0.37),P < 0.01],but higher than that of WKY
      group(0.81±0.22,P < 0.05). Conclusion Olmesartan could singificantly
      reduce the leve of AT1 receptor mRNA in RVLM of the SHR.

Key words: rostral ventrolateral medulla, spontaneously hypertensive rat,
     ,
angiotensinⅡtype 1 receptor, olmesartan

[1] 吴荣,马天馨,曾越灿. 血管紧张素Ⅱ1型受体拮抗剂在大鼠放射性心脏损伤中保护作用的研究[J]. 中国医科大学学报, 2015, 44(1): 20-26.
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