中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2010, Vol. 39 ›› Issue (1): 1–.

• 基础医学 •    下一篇

鼠输尿管完全梗阻后肾脏病理及足细胞表型改变的研究

于雪馨; 王常林; 孙荣国; 杨屹   

  1. 中国医科大学附属盛京医院小儿内科; 中国医科大学附属盛京医院小儿泌尿外科
  • 出版日期:2010-01-20 发布日期:2013-06-07
  • 通讯作者: 杨屹 E-mail:yangy2@sj hospital.org

Renal Pathological Appearance and Podocyte Phenotype after the Ureteral Obstruction

  • Online:2010-01-20 Published:2013-06-07

摘要:

目的观察鼠输尿管完全梗阻后肾脏病理和足细胞表型改变的时序变化,寻找输尿管梗阻后肾脏发生不可逆损伤的时相。方法建立单侧输尿管完全梗阻动物模型,梗阻后不同时间点取出肾脏,经HE染色和免疫荧光双标方法,观察肾脏病理及足细胞表型的改变。结果大体观察,梗阻24h后出现肾盂扩张,梗阻3d后出现肾皮质变薄。组织学观察,梗阻12h后出现一部分近曲小管扩张,小管上皮细胞呈水样变性肿胀,在梗阻3d后出现大量小管上皮细胞变形肿胀。免疫荧光双标法,梗阻24h后有少量足细胞、巨噬细胞同时被2种抗体标记,2d后足细胞呈减少趋势,而巨噬细胞逐渐增多。结论输尿管梗阻后在肾脏病理改变早于肾脏发生形态学改变的发生,输尿管梗阻后24h发生足细胞表型的改变,与肾脏肾盂出现明显扩张的时间相近,随着梗阻时间的延长,肾脏病理改变逐渐加重,足细胞转化逐渐增多。

关键词: 输尿管梗阻, 足细胞表型, 肾脏损伤, 输尿管梗阻, 足细胞表型, 肾脏损伤

Abstract:

Objective To find time point of the irreversible kidney injury by
      observing renal pathological appearance and podocytes phenotype after
      complete ureteral obstruction. Methods The animal model of complete
      unilateral ureteral obstruction was constructed in 2-weekold and
      2-month-old rats respectively. HE staining was employed to evaluate the
      degree of pathological changes,and immunofluorescence double staining to
      observe the transitional progression of podocyte. Results Grossly,there
      appeared pyelectasis at 2 h after obstruction and thinner renal cortex
      after 3 days of obstruction. Histologically,proximal tube display dilated
      and hydropic degeneration of tubular epithelial cells was observed after
      12-day obstruction. The tubular epithelium showed distorted swelling after
      3-day obstruction. Immunofluorescence double staining showed that
      podocytes decreased and the double-labeled macrophages was increased
      during 1 d to 2 d after unilateral ureteral obstruction operation.
      Conclusion Compared with control groups,the unilateral ureteral
      obstruction groups shows podocyte-to-macrophage transition at 1 d after
      the operation,while the pelvis shows obviously dilated. From the 1st day
      to the 7th day after unilateral ureteral obstruction,it shows increasing
      tendency of the podocytes transition.

Key words: unilateral ureteral obstruction, podocyte cell transition, renal injury, Unilateral ureteral obstruction, Podocyte Cell transition, Renal injury

[1] 于方,邓海月,姜红. 咪唑立宾对单侧输尿管梗阻小鼠肾小管上皮间质转化的影响[J]. 中国医科大学学报, 2016, 45(8): 728-732.
[2] 薛东炜,刘春来,张西玲,王平. 肿瘤用输尿管支架管治疗恶性输尿管梗阻的临床应用[J]. 中国医科大学学报, 2014, 43(5): 468-469.
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