中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2010, Vol. 39 ›› Issue (1): 14–.

• 基础医学 • 上一篇    下一篇

大鼠神经干细胞中MAPKK/MEK-MAPK/ERK信号转导通路的实验研究

赵宇; 谢鹏; 朱晓峰; 蔡志友   

  1. 重庆医科大学附属第一医院神经内科; 佳木斯大学神经科学研究所
  • 出版日期:2010-01-20 发布日期:2013-06-07
  • 通讯作者: 赵宇 E-mail:wangpang0916@yahoo.com.cn

Effect of MAPKK/MEK-MAPK/ERK Signal Transduction Pathway in Rat Neural Stem Cells

  • Online:2010-01-20 Published:2013-06-07

摘要:

目的应用丝裂原激活的蛋白激酶激酶(MAPKK/MEK)特异性阻滞剂PD98059孵育大鼠神经干细胞(NSC),探讨MAPKK/MEK-MAPK/ERK信号通路在NSC中的作用。方法体外分离、培养大鼠NSC,应用不同浓度的PD98059孵育NSC后进行WTS-8、Nestin,BrdU、β-tubulin-Ⅲ、Western
      blot检测。结果PD98059对NSC的存活、增殖和分化的影响呈剂量依赖性,在PD98059较高浓度时NSC的存活率明显下降(P<0.05),BrdU和β-tubulin-Ⅲ的阳性细胞数明显少于正常对照组(P<0.05)。PD98059阻断ERK表达的作用呈剂量依赖性。结论MAPKK/MEK-MAPK/ERK信号通路对大鼠NSC的存活、增殖、分化起重要作用。

关键词: 丝裂原激活的蛋白激酶激酶, 细胞外信号调控激酶, 神经干细胞, 丝裂原激活的蛋白激酶激酶, 细胞外信号调控激酶, 神经干细胞

Abstract:

Objective To incubate the rat neural stem cells with specific
      inhibitor(PD98059)of MAPKK/MEK to clarify the effect of MAPKK/MEK-MAPK/ERK
      signaling pathway in neural stem cells (NSC). Methods NSCs derived from
      E15-16 rats were isolated and cultured. After treated with different
      concentration of PD98059,they were subjected to the detection of cell
      proliferation,Nestin,BrdU and β- tubulin-Ⅲ by WTS-8
      assay,immunofluorescent staining or Western Blot respectively. Results
      PD98059 had an effect on the survival,proliferation and differentiation of
      NSC at a concentration-dependent manner. The viability of the NSC was
      significantly decreased than the control(P < 0.05),whereas the numbers of
      BrdU-positive and β-tubulin-Ⅲ positive cells were notably fewer than the
      control(P < 0.05)after incubated with the higher concentration of PD98059.
      The ERK expression was blocked by PD98059 at a concentration-dependent
      manner. Conclusion The MAPKK/MEK-MAPK/ERK signaling pathway played a vital
      role in the survival,proliferation and differentiation of NSC.

Key words: mitogen activated protein kinases kinases, extracellular signal regulated kinase, neural stem cell

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[2] 闫荣,罗晓光,张尧,李宇凤冯娟. 星形胶质细胞影响神经干细胞突触表达的神经营养家族基因表达机制探讨[J]. 中国医科大学学报, 2013, 42(11): 989-995.
[3] 刘欣春,朱悦. 神经干细胞与骨骼肌细胞的共同培养[J]. 中国医科大学学报, 2012, 41(1): 21-23.
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