中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2010, Vol. 39 ›› Issue (1): 22–.

• 基础医学 • 上一篇    下一篇

兔脑创伤后早期血清β淀粉样前体蛋白动态变化的实验研究

黄海能; 罗起胜; 赵邦; 谭源福; 邓元央; 黄华东; 符黄德; 李传玉   

  1. 广西右江民族医学院附属医院神经外科; 广西医科大学附属第一医院神经外科
  • 出版日期:2010-01-20 发布日期:2013-06-07
  • 通讯作者: 黄海能 E-mail:hnhuang@126.com

The Dynamical Changes of Serum β-APP in Early Stage of Rabbit Traumatic Brain Injury

  • Online:2010-01-20 Published:2013-06-07

摘要:

目的探讨兔脑创伤后血清β淀粉样前体蛋白(β-APP)含量与损伤程度及其时间动态变化相关性。方法将24只大白兔随机分为对照组和轻、中、重度脑损伤组,各组于不同时间点(0.5h、2h、6h、24h)采集标本,检测血清β-APP的含量。结果采用完全随机设计多因素重复测量方差分析和组间两两比较的LSD检验进行统计学分析。结果脑损伤后血清β-APP均升高。中、重度损伤组各时间点血清β-APP含量较对照组和轻度损伤组升高(P<0.05),重度损伤组血清β-APP含量各时间点均高于同时间点的中度损伤组,但差异无统计学意义(P>0.05);轻度损伤组血清β-APP含量与对照组相比在统计学上无显著性差异(P>0.05)。结论脑损伤后血清β-APP含量升高,且损伤越重血清β-APP含量越高。血清β-APP含量可作为早期判断颅脑损伤程度指标之一。

关键词: 创伤性脑损伤, 酶联免疫吸附实验法, β淀粉样前体蛋白

Abstract:

Objective To explore the relationship of serum levels of β-amyloid
      precursor protein (β-APP)with degree of traumatic brain injury (TBI)and
      the traumatic time. Methods Sprague-Dawley (SD)rats were randomly divided
      into normal control and injury group. The rats in injury groups suffered
      from TBI after free-falling percussion with different
      pressure(wild-injury,moderate-injury and severe-injury group). Then serum
      was collected at 0.5 h,2 h,6 h,and 24 h and subject to β-APP detection by
      ELISA. All data were analyzed statistically with completely randomized
      design multiple factor repeated measure of variance analysis and least
      significant difference (LSD)test. Results The serum levels of β-APP were
      higher after injury. The serum levels of β-APP were significantly higher
      in moderate-injury or severe-injury group than those in normal group or
      slight-injury group(P < 0.05). The serum levels of β-APP were higher in
      severe-injury group than that in moderate-injury group with no statistical
      difference (P > 0.05). There was no statistical difference in serum
      β-APP levels between normal control and slight-injury group (P > 0.05).
      Conclusion The serum level of β-APP is increasingly higher with traumatic
      brain injury more serious and could be employed as an indicator of TBI
      degree. It implies that β-APP has the potential as an early diagnosis
      marker for TBI.

Key words: traumatic brain injury, enzyme linked immunosorbent assay; β-amyloid precursor protein

[1] 李力卓 ,何松柏. 大鼠创伤性脑损伤后精神障碍的精神行为学改变[J]. 中国医科大学学报, 2016, 45(8): 708-710.
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