中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2010, Vol. 39 ›› Issue (1): 28–.

• 口腔医学 • 上一篇    下一篇

一种新型可切削生物活性玻璃陶瓷的微核实验

郝玉全; 阎秀林; 张旖骁; 韩雪松; 刘敏达; 艾红军   

  1. 中国医科大学口腔医学院口腔修复科; 中国医科大学口腔医学院口腔正畸科; 中国医科大学第94期临床医学系; 武警辽宁省总队医院口腔科; 中国医科大学第93期口腔医学系
  • 出版日期:2010-01-20 发布日期:2013-06-07
  • 通讯作者: 艾红军 E-mail:aih0620@yahoo.com.cn

Micronucleus Test of a New Machinable Bioactive Glass-ceramic Material

  • Online:2010-01-20 Published:2013-06-07

摘要:

目的评价一种新型可切削生物活性玻璃陶瓷的潜在致突变性。方法将30只N1H纯系小鼠随机等分为玻璃陶瓷组、阴性对照组和阳性对照组。玻璃陶瓷组将待测样品制成粉末状,用阿拉伯胶将其配成混悬液,以5g/kg的剂量经口灌胃;阴性对照组和阳性对照组分别灌注等体积的阿拉伯胶和环磷酰胺生理盐水溶液(40mg/kg)。取小鼠骨髓细胞,油镜下每只动物观察1000个嗜多染红细胞,并计算微核率。结果玻璃陶瓷组、阴性对照组和阳性对照组的微核率分别为(1.31±0.53)‰、(1.32±0.62)‰和(29.20±0.74)‰,玻璃陶瓷组与阴性对照组的差异无统计学意义,与阳性对照组的差异有统计学意义(P<0.01)。结论该新型可切削生物活性玻璃陶瓷不引起小鼠骨髓细胞微核率增加。

关键词: 玻璃陶瓷, 微核实验, 致突变性, 玻璃陶瓷, 微核实验, 致突变性

Abstract:

Objective To evaluate the potential mutagenicity of a new
      machinable bioactive glass-ceramic material. Methods Thirty N1H mouse
      inbred line(female:male =1:1)were divided to three groups at random(n
      =10),including glass-ceramic groups(oral administration of 5 g/kg
      glass-ceramic powder and arabic gum),negative control group(arabic gum in
      equal volume),and positive control group(oral administration of 40 mg/kg
      cyclophosphamide). The mice orally intook the equivalent liquor and were
      sacrificed with bone marrow cells abstracted 24 hours later. The
      micronucleated cells were counted in 1 000 polychromatic erythrocytes
      (PCE)per mouse,then the rate of the micronucleus in every group was
      measured. Results The rate of the micronucleus in glass-ceramic
      group,negative control group and positive control group was
      1.31±0.53‰,1.32±0.62‰ and 29.20±0.74‰ respectively. There was no
      significant difference in the rate of the micronucleus between the
      experimental and negative groups (P > 0.05),while a significant
      difference in the rate of the micronucleus was observed between
      experimental and positive groups(P < 0.01). Conclusion The new machinable
      bioactive glass-ceramic materials couldn’t increase the micronucleus rate
      of mouse bone marrow cells.

Key words: glass-ceramic, micronucleus test, mutagenicity, glass-ceramic, micronucleus test, mutagenicity

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