中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2010, Vol. 39 ›› Issue (2): 112–.

• 基础医学 • 上一篇    下一篇

丝胶预处理对DN大鼠肾脏细胞外基质相关蛋白表达的影响

陈志宏; 宋成军; 付秀美; 付文亮; 薛景凤   

  1. 承德医学院基础医学部解剖学教研室
  • 出版日期:2010-02-27 发布日期:2013-06-07
  • 通讯作者: 薛景凤 E-mail:xjf@cdmc.edu.cn

Effects of Sericin Pretreatment on the Expression of ECM Associated Protein in the Kidney of Diabetic Nephropathy Rats

  • Online:2010-02-27 Published:2013-06-07

摘要:

目的探讨彩色蚕茧提取物—丝胶预处理对糖尿病肾病(DN)大鼠肾脏细胞外基质(ECM)相关蛋白表达的影响。方法36只雄性SD大鼠随机分为3组(每组12只):正常对照组、DN模型组和丝胶预防组。模型组、丝胶预防组大鼠均建立链脲佐菌素(STZ)致动物模型,以血糖≥16.7mmol/L为成模标准;丝胶预防组大鼠于注射STZ前给与丝胶(2.4g·kg-1·d-1)灌胃35d。采用酶法检测血糖,ELISA方法检测血清Ⅳ型胶原(cⅣ)和层黏蛋白(LN)的含量;免疫组化染色观察肾脏转化生长因子-β1(TGF-β1)和基质金属蛋白酶组织抑制因子-1(TIMP-1)蛋白的表达;Westernblot法观察肾脏Smad3蛋白的表达。结果与正常对照组大鼠相比,模型组大鼠血糖,血清cⅣ和LN含量,肾脏TGF-β1、TIMP-1和Smad3蛋白的表达均明显升高(P<0.01)。丝胶预防组大鼠的血糖、血清cⅣ和LN含量,肾脏TGF-β1、TIMP-1和Smad3蛋白的表达明显低于模型组(P<0.01)。结论丝胶预处理可抑制DN肾脏中TGF-β1/Smad3信号通路的激活,防止TIMP-1蛋白高表达导致的MMPs活性的降低,从而预防DN时ECM的积聚和肾小球硬化,对DN的发生发展具有良好的预防作用。

关键词: 丝胶, 预处理, 糖尿病肾病, 细胞外基质, 转化生长因子-β1, Smad3, 基质金属蛋白酶组织抑制因子-1, 丝胶, 预处理, 糖尿病肾病, 细胞外基质, TGF-β1, Smad3, TIMP-1

Abstract:

Objective To investigate the effects of sericin pretreatment on the
      expression of extracellular matrix(ECM)associated protein in diabetic
      nephropathy(DN)rats'kidney.Methods Sixty six male SD rats were randomly
      divided into 3 groups(n=12):normal control group,DN model group and
      sericine pretreatment group.DN rats model in model group and sericine
      pretreatment group were established by intraperitoneally injection of
      streptozotocin(STZ).Blood glucose≥16.7 mmol/L was taken as the standard of
      successful modelization.The rats in sericine pretreatment group were
      lavaged with sericine(2.4 g·kg-1·d-1)for 35 days before injecting STZ.The
      enzymic method was used to measure the blood glucose.Type Ⅳ
      collagen(cⅣ)and laminin(LN)content in the serum were detected by ELISA.The
      expression of transforming growth factor-β1(TGF-β1)and tissue inhibitors
      of maprix metalloproteinase-1(TIMP-1)protein in the kidney was observed by
      immunohistochemical staining.The expression of Smad 3 protein in the
      kidney was detected by Western blot.Results Compared with normal
      control rats,the blood glucose,cⅣ and LN content in the
      serum,TGF-β1,TIMP-1 and Smad 3 expression in the kidney of the model group
      rats increased obviously(P < 0.01).The blood glucose,cⅣ and LN content in
      the serum,TGF-β1,TIMP-1 and Smad3 expression in the kidney of rats in
      sericine pretreatment group were significantly lower than those of the
      rats in model group(P < 0.01).Conclusion Sericin pretreatment can inhibit
      the activation of TGF-β1/Smad 3 signal pathway in the kidney of DN
      rats,and prevent the decrease of MMPs activity induced by up-regulation of
      TIMP-1.So sericin can prevent accumulation of ECM and glomerulosclerosis
      during DN,and has satisfactory apotropaic effects on the development of
      DN.

Key words: sericin, petreatment, dabetic nephropathy, etracellular matrix; TGF-β1, Smad 3, TIMP-1, Sericin, Pretreatment, Diabetic nephropathy, Extracellular matrix, TGF-β1, Smad3, TIMP-1

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