中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2010, Vol. 39 ›› Issue (2): 128–.

• 临床医学 • 上一篇    下一篇

野生型p53基因调控膀胱移行细胞癌中心体异常的实验研究

王明; 王连渠; 谭毅   

  1. 重庆医科大学附属第一医院泌尿外科
  • 出版日期:2010-02-27 发布日期:2013-06-07
  • 通讯作者: 王明 E-mail:wangming1963666@163.com

Effect of Wild Type p53 Gene on Inhibiting the Centrosome Hyperamplification in Bladder Cancer Cell Line

  • Online:2010-02-27 Published:2013-06-07

摘要:

目的研究野生型p53基因抑制膀胱癌细胞中心体异常的作用。方法将野生型p53基因重组腺病毒载体转染人膀胱癌细胞T24,应用免疫荧光化学法检测膀胱癌细胞中心体异常变化情况。结果野生型p53基因可抑制膀胱移行细胞癌T24细胞中心体异常。结论p53基因可能是膀胱移行细胞癌中心体异常的一个重要调控因子,p53基因失活可能引起肿瘤细胞染色体不稳,从而促进肿瘤的发生和发展。

关键词: 野生型p53基因, 膀胱癌细胞株, 中心体异常, 染色体不稳

Abstract:

Objective To study the effect of wild type p53 gene on centrosome
      hyperamplification in bladder cancer cells.Methods A wild type p53 gene
      recombinant adenovirus vector AdCMVp53 was constructed,and then
      transfected into the human bladder cancer cell line T24.The cells were
      stained with the monoclonal antibody against pericentrin by indirect
      immunofluorescence method.The change of centrosome hyperamplification was
      observed under the fluorescence microspcope.Results Introduction of wild
      type p53 could suppress the centrosome amplification of T24 cell
      line.Conclusion p53 might play an important role in the regulation of
      centrosome hyperamplification.The loss of p53 might be one of the
      mechanisms involved in chromosome instability and contribute to the
      genesis and development of the bladder carcinoma.

Key words: wild type p53 gene, bladder cancer cell line, centrosome hyperamplification, chromosome instability

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