中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2010, Vol. 39 ›› Issue (2): 132–.

• 临床医学 • 上一篇    下一篇

阿司匹林对人子宫内膜腺癌Ishikawa细胞生长和凋亡的影响

赵冬妮; 金英楠; 李艳霞; 马晓欣   

  1. 中国医科大学附属盛京医院妇产科; 北京市西城区疾病预防控制中心生殖保健科
  • 出版日期:2010-02-27 发布日期:2013-06-07
  • 通讯作者: 马晓欣 E-mail:maxiaoxin666@yahoo.com.cn

Effects of Aspirin on the Proliferation and Apoptosis of Human Endometrial Adenocarcinoma Ishikawa Cells

  • Online:2010-02-27 Published:2013-06-07

摘要:

目的研究阿司匹林对体外培养的人子宫内膜腺癌Ishikawa细胞增殖和凋亡的影响。方法采用MTT法检测不同浓度及作用时间下阿司匹林对子宫内膜腺癌Ishikawa细胞增殖的影响;通过流式细胞仪检测不同浓度阿司匹林对细胞周期和凋亡率的影响;应用透射电镜观察阿司匹林作用后细胞的形态学变化。结果阿司匹林对人子宫内膜腺癌Ishikawa细胞的增殖具有明显的抑制作用,呈时间与剂量依赖性(P<0.05);阿司匹林使细胞阻滞在G1期,细胞凋亡率增加,与药物作用浓度相关(P<0.05);阿司匹林作用后细胞体积缩小,染色质浓缩致密,可见凋亡小体形成。结论阿司匹林能够显著抑制人子宫内膜腺癌Ishikawa细胞的增殖,并诱导其发生凋亡。

关键词: 阿司匹林, Ishikawa细胞, 肿瘤生长, 子宫内膜腺癌, [关键词] 阿司匹林, Ishikawa细胞, 肿瘤生长

Abstract:

Objective To assess the effects of aspirin on the proliferation and
      apoptosis of human endometrial adenocarcinoma cells.Methods MTT assay was
      used to measure the effects of aspirin on the proliferation of Ishikawa
      cell.Flow cytometry(FCM)was employed to examine the distribution of cell
      cycles and the rates of apoptosis.Transmission electron microscopy was
      used to observe cell morphologic changes after aspirin
      administration.Results Aspirin inhibited the proliferation of cultured
      Ishikawa cells in a time-dependent and dosedependent manne(rP <
      0.05).Aspirin increased the distribution of G1 stage and the rates of cell
      apoptosis in a dose-dependent manne(rP < 0.05).Morphologic features of
      apoptosis cells,including cell shrinkage,nuclear condensation and
      apoptotic bodies could be found obviously under the transmission electron
      microscopy.Conclusion Aspirin inhibited the proliferation and increased
      the apoptosis of human endometrial adenocarcinoma Ishikawa cells.

Key words: aspirin, Ishikawa cell, tumor growth, endometrial adenocarcinoma, [Key words] Aspirin, Ishikawa cells, tumor growth

[1] 杜岩,曲华. 负荷量氯吡格雷联合阿司匹林治疗再发性脑梗死的临床研究[J]. 中国医科大学学报, 2017, 46(9): 836-838.
[2] 苗鹏, 关琳, 邢承忠. 服用阿司匹林对结直肠癌患者生存获益的Meta分析与系统评价[J]. 中国医科大学学报, 2015, 44(6): 528-532.
[3] 吕红伟,马晓欣,柯孝瑜,李艳霞. 多西他赛对人子宫内膜腺癌Ishikawa细胞凋亡诱导的实验研究[J]. 中国医科大学学报, 2012, 41(8): 716-718.
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