中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2010, Vol. 39 ›› Issue (2): 95–.

• 基础医学 • 上一篇    下一篇

NK细胞杀瘤活性与肿瘤细胞表面MICA/B表达的相关性研究

王新利; 李卿; 蒋涛; 王扬; 付立叶; 高婷; 姜又红   

  1. 中国医科大学附属第一医院肿瘤研究所第二研究室
  • 出版日期:2010-02-27 发布日期:2013-06-07
  • 通讯作者: 姜又红 E-mail:jiangyouhong2000@yahoo.com.cn

The Relationship between the MICA/B Expression on Cancer Cell Lines and Their Sensitivity to NK Cell Cytotoxicity

  • Online:2010-02-27 Published:2013-06-07

摘要:

目的探讨自然杀伤NK细胞杀瘤活性与肿瘤细胞表面MICA/B表达的相关性。方法采用流式细胞术检测人红白血病细胞K562、乳腺癌细胞Bcap37、肾癌细胞769P及肺癌细胞A549表面MICA/B的表达,RosetteSep誖改进法分离人外周血NK细胞,MTT法检测不同效靶比时NK细胞对几种肿瘤细胞的杀伤活性。结果K562、Bcap37、769P及A549细胞表面MICA/B的表达阳性率分别是(60.35±0.50)%、(90.72±0.64)%、(55.59±0.55)%、(3.84±0.10)%;RosetteSep誖改进法分选出的NK细胞纯度为(96.52±2.42)%,在同一效靶比时,NK细胞对K562、Bcap37及769P的杀伤活性较强,与A549相比有显著性差异(P<0.01),其中K562与Bcap37之间无显著性差异(P>0.05);效靶比为5∶1、10∶1、20∶1时,NK细胞的杀伤活性与4种肿瘤细胞表面MICA/B表达均呈正相关关系(P<0.01),随着效靶比增加,相关性增强。结论NK细胞的杀伤敏感性与肿瘤细胞表面MICA/B的表达水平相关,肿瘤细胞表面MICA/B的表达水平影响NK细胞的杀伤活性。

关键词: 自然杀伤细胞, 肿瘤细胞, MICA/B, 细胞毒性实验, NK细胞;肿瘤细胞;MICA/B;细胞毒性实验;

Abstract:

Objective To analyze the relationship between the expression of MHC
      class I chain-related gene A/B(MICA/B) on different human cancer cells and
      their sensitivity to NK cell cytotoxicity.Methods The expression MICA/B in
      K562,Bcap37,769P and A549 cells was measured by FACS.Isolation of NK cells
      were obtained by the modified RosetteSep procedure.Cytotoxicity of NK
      cells at different effect-to-target ratios(E:T)were determined by MTT
      assay.Results The expression of MICA/MICB on 4 tumor cell lines
      were(60.35± 0.50)%,(90.72±0.64)%,(55.59±0.55)%,and(3.84±0.10)%
      respectively.The purity of NK cells obtained by the modified Rosette Sep
      procedure was(96.52±2.42)%,whereas the cytotoxicity of NK cells against
      K562,Bcap39 and 769P was much higher than that of A549(P < 0.01).The
      expression of MICA/B was significantly correlated with the cytotoxicity of
      NK cells at E:T ratios of 5∶1,10∶1 and 20∶1 respectively(P
      <0.01).Conclusion The MICA/B expression on cancer cell lines was
      correlated with NK cell-mediated cytolysis and influenced the
      cytotoxicity of NK cells.

Key words: natural killer cell, tumor cell lines, MICA/B, cytotoxicity, Natural killer cell;tumor cell lines; MICA/B;Cytotoxicity;

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