中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2012, Vol. 41 ›› Issue (1): 1–4.

• 基础医学 •    下一篇

创伤后应激障碍大鼠动眼神经核神经元5-羟色胺1A受体表达的变化

刘冬娟1,韩芳2,肖冰1,罗斐斐2,徐盟3,王恩华4,石玉秀1,2   

  1. (中国医科大学1. 基础医学院电子显微学研究室;2. 基础医学院组织胚胎学教研室;3. 第95期临床医学七年制;4. 病理与病理生理学研究所,沈阳 110001)
  • 收稿日期:2012-09-19 修回日期:2012-09-19 出版日期:2012-01-01 发布日期:2012-09-21
  • 通讯作者: 石玉秀,E-mail: shiyuxiu@163.com
  • 作者简介:刘冬娟(1962-),女,副主任技师,本科.
  • 基金资助:
    国家自然科学基金资助项目(31140060;81171282);国家教育部博士点基金资助项目(20092104110016)

The Expression of 5-HT1A Receptor in the Oculomotor Nucleus of Posttraumatic Stress Disorder Rats

LIU Dong-juan1, HAN Fang2, XIAO Bing1, LUO Fei-fei2,XU Meng3,WANG En-hua4, SHI Yu-xiu1,2   

  1. (1. Department of Electron Micrology, College of Basic Medical Sciences, China Medical University; 2. Department of Histology and Embryology, College of Basic Medical Sciences, China Medical University; 3. The 95th Class, 7-year System, Faculty of Clinical Medicine, China Medical University; 4. Institute of Pathology and Pathophysiology, China Medical University, Shenyang 110001, China)
  • Received:2012-09-19 Revised:2012-09-19 Online:2012-01-01 Published:2012-09-21

摘要: 目的 观察创伤后应激障碍(PTSD)大鼠动眼神经核神经元5-羟色胺1A (5-HT1A)受体的表达变化。方法 采用无连续单一刺激(SPS)方法,建立PTSD样大鼠SPS模型,随机分为SPS后1 d、4 d和7 d组及正常对照组。应用免疫组化、Western blot和透射电镜技术,检测动眼神经核神经元5-HT1A受体的表达变化和观察神经元超微结构的改变。结果 SPS后大鼠动眼神经核神经元5-HT1A受体的表达高于正常对照组,尤以SPS后7 d组最为明显;神经元超微结构也发生改变。结论 SPS刺激可促进大鼠动眼神经核神经元5-HT1A受体的表达增强,进而导致神经递质紊乱,影响动眼神经核支配眼球肌的运动功能出现相应的PTSD症状。

关键词: 创伤后应激障碍, 动眼神经核, 5-HT1A受体, 神经元, 免疫组化

Abstract: AbtractObjective To observe the change of 5-HT1A receptor-expression in the oculomotor nucleus of posttraumatic stress disorder rats. Methods The SPS-method was used to set up the rat PTSD models. Rats were randomly divided into 1d ,4d and 7d groups of single prolong stress (SPS) and a normal group. Immunohistochemical and Western blot were used to detect the expression of receptor (5-HT1AR) in the oculomotor nucleus. The change of ultrastructure in the neuron were objected by transmission electron microscopy (TEM). Results The expression of 5-HT1AR in the oculomotor nucleus neurons gradually increased on 1 d, 4 d and 7 d after exposure to SPS than that of the control group, and reached the peak level at 7d after SPS exposure. There was also a change of ultrastructure in the oculomotor nucleus neuron detected. Conclusion SPS exposure resulted in the change of 5-HT1A receptor- expression in the oculomotor nucleus, which might be related to the pathogenesis of PTSD.

Key words: Key words posttraumatic stress disorder, oculomotor nucleus, 5-HT1A receptor, neuron, immunohistochemistry

中图分类号: 

  • R749.5
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