中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2012, Vol. 41 ›› Issue (1): 28–30.

• 基础医学 • 上一篇    下一篇

白藜芦醇对帕金森病大鼠黑质多巴胺能神经元损伤的保护作用

王聪杰,牛平,陈鑫,刘宝茹,辛志强   

  1. (沈阳军区总医院神经内科,沈阳 110016)
  • 收稿日期:2012-09-19 修回日期:2012-09-19 出版日期:2012-01-01 发布日期:2012-09-21
  • 通讯作者: 牛平,E-mail:np0702@sina.com
  • 作者简介:王聪杰(1982-),男,主治医师,硕士.
  • 基金资助:
    辽宁省自然科学基金资助项目(20052072)

The Study on The Neuroprotective Effects of Resveratrol Against Degeneration of Dopaminergic Neurons in Parkinson’s Disease Rat Model

WANG Cong-jie,NIU Ping, CHEN Xin ,LIU Bao-ru,XIN Zhi-qiang.   

  1. (Department of Neurology, General Hospital of Shenyang Military Command, Shenyang 110016, China)
  • Received:2012-09-19 Revised:2012-09-19 Online:2012-01-01 Published:2012-09-21

摘要: 目的 观察白藜芦醇(Res)对脂多糖(LPS)所致大鼠黑质多巴胺能(DA)神经元损伤的保护作用。方法 黑质内注射LPS制作帕金森病(PD)大鼠模型。应用Res对实验动物进行处理。通过行为学、酪氨酸羟化酶(TH)、核因子κB(NF-κB)等免疫组化及免疫印迹技术观察Res对DA能神经元损伤的作用。结果 对照组大鼠无行为学变化,PD组大鼠平均旋转圈数为(196.90±9.52)圈,Res组为(106.57±7.89)圈,差异有统计学意义(P<0.01)。TH免疫组化结果表明,对照组大鼠黑质TH阳性神经元数量较多,胞体较大,突起明显;PD组神经元数量明显减少(P<0.01),甚至消失,神经元胞体萎缩,突起亦不清晰。Res组TH阳性神经元数量与PD组相比明显增加(P<0.01),神经元形态变化亦不明显。NF-κB免疫组化结果表明,对照组黑质仅见少数NF-κB阳性细胞,无明显核转位现象;PD组黑质NF-κB阳性细胞较多,胞质呈黄褐色,部分细胞核也着色,有明显核转位现象;与PD组相比,Res组NF-κB阳性细胞数明显减少(P<0.01)。小胶质细胞特异性抗体(OX-42)免疫组化结果表明,对照组大鼠黑质小胶质细胞多呈静止的“分枝样”状态,PD组多为激活状态,呈典型的“阿米巴样”;Res组激活状态的小胶质细胞与PD组相比明显减少,形态介于静止和激活状态之间。Western blot分析结果显示,与对照组相比,PD组TH蛋白表达减少,NF-κB P65蛋白表达明显增高(P<0.01);与PD组相比,Res组TH蛋白表达增加,NF-κB P65蛋白表达显著下降(P<0.01)。结论 Res可对LPS所致黑质DA能神经元损伤起防护作用,具有抗炎及神经保护作用。

关键词: 白藜芦醇, 多巴胺能神经元, 脂多糖

Abstract: Objective To observe the neuroprotective effects of Resveratrol (Res) against degeneration of dopaminergic neurons which caused by LPS in vivo. Methods PD rat model were made by intranigral injection of LPS..Sprague-Dawley rats were randomly divided into control group ,PD group and Res group. Behaviour study, tyrosine hydroxylase(TH) and nuclear factor kappa B(NF – κB), Immuno-histochemistry and Western blotting were performed. Results The control group hadn’t shown any rotational behavior, the number of circling behavior in PD group was the most in all groups(196.90±9.52). there was significant differences compared with control group and Res group (P<0.01).The number of TH positive cells and the expression of TH protein in substantial nigra of PD group were significantly decreased compared with control group(P<0.01);AT the same time, the NF – κB positive cells and NF – κB protein expression were up regulated and showed the most of microglias activation. But compared with PD group,the number of TH positive cells and TH protein expression was increased ,the NF – κB positive cells and NF – κB protein expression was decreased in Res group (P<0.01). Conclusion Res showed neuroprotective effects and could lessen the degeneration of dopaminergic neurons caused by LPS in vivo.

Key words: resveratrol, dopaminergic neuron, lipopolysaccharide

中图分类号: 

  • R284.1
[1] AaBurkitt MJ, Duncan J. Effects of trans-resceratrol on copper-de-pendent hvdroxyl-radical formation and DNA damage; evidence for hydroxyl-radical seavenging and a novel, glutathione-sparing mechanism of action[J]. Arch Biochem Biophys, 2000, 381(2): 253-263.
[2]Lin YL, Chang HL, Chen TL,et al. Resveratrol protects against oxidized LDL-induced breakage of the blood-brain barrier by leasening disruption of tight junctions and apoptotic insults to mouse cerebrovascular endothelial cells[J]. J Nutr,2010,140(12):2181-2192.
[3] Tanka H, Yokota H, Jover T, et a1. Iscbemic preconditioning: neurona1 survival in the face of caspase-3 activation[J]. J Neuroscience, 2004, 24(11): 2750-2759.
[4] Panet J, Barzilai A, Daily D, et al. Activation of nuclear transcription factor kappa B is essential for dopamine-Induced apoptosis in PC12 cells[J]. J Neurochem, 2001, 77(2): 391-398.
[5] Hunot S, Brugg B, Ricard D, et al. Nuclear translocation of NF-κB is increased indopaminergic neurons of patients with Parkinson disease[J]. Proc Natl Acad Sci. USA, 1997, 94(1): 7531–7536.
[6] Leiro J, Arran JA, Frai N, et al. Effect of cis-resveratrol on genes involved in nuclear actor kappa B signaling[J]. Int Immuropharmacol, 2005, 5(2): 393-406.
[7] Donnelly LE, Newton R, Kennedy GE, et al. Anti-inflammatory effects of resveratrol in lung epithelial cells: molecular mechanisms[J]. Am J Physiol Lung Cell Mol Physiol, 2004, 287(4): L774-L783.
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