中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2012, Vol. 41 ›› Issue (2): 104–107.

• 基础医学 • 上一篇    下一篇

环氧合酶2介导姜黄素对慢性脂多糖诱导的脊髓突触可塑性损害的拮抗作用

杨红卫1, 王翔宇2   

  1. 三峡大学1.医学院生理学教研室,湖北 宜昌 443002;2.第三临床学院葛洲坝中心医院神经内科,湖北 宜昌 443000
  • 收稿日期:2012-09-20 修回日期:2012-09-20 出版日期:2012-02-20 发布日期:2012-09-21

Cyclooxygenase-2 Mediated Antagonism Effect of Curcumin on Chronic Exposure to LPS -Induced Impairment of Spinal Synaptic Plasticity

YANG Hong-Wei1, WANG Xiang-Yu 2   

  1. 1. Department of Physiology, College of Medical Science of China Three Gorges University, Yichang 443002, China;2. Department of Neurology, Gezhouba Central Hospital, Third Clinical Hospital of China Three Gorges University, Yichang 443000, China
  • Received:2012-09-20 Revised:2012-09-20 Online:2012-02-20 Published:2012-09-21

摘要: 目的 探讨环氧合酶2 (COX-2)与姜黄素(Cur)对慢性脂多糖(LPS)诱导的脊髓背角C-纤维诱发电位长时程增强(LTP)的作用。方法 分别给予大鼠腹腔注射LPS (3 mg/kg)、Cur(300 mg/kg)、Cur(300 mg/kg)或COX-2的选择性抑制剂NS398 (10 mg/kg)联合LPS (3 mg/kg) 7 d后,在脊髓腰膨大部记录背角浅层神经元C-纤维诱发电位,观察COX-2及Cur对脊髓LTP的效应,采用蛋白质印迹法检测不同条件下COX-2蛋白的表达。结果 慢性给予LPS可显著降低脊髓LTP,此效应可被Cur所抑制。Cur本身并不影响脊髓LTP。LPS降低脊髓LTP的效应亦可被COX-2的选择性抑制剂NS398所翻转。Cur及NS398均可显著抑制LPS诱导的脊髓COX-2蛋白表达的增强。结论 Cur对LPS诱导的脊髓LTP损害的保护作用可能是通过COX-2介导的。

关键词: 环氧合酶2, 姜黄素, 脂多糖, 长时程增强, 脊髓

Abstract: Objective To explore the role of cyclooxygenase-2 (COX-2) and Curcumin on chronic exposure to lipopolysaccharide (LPS)- induced inhibition of spinal long-term potentiation (LTP) is evaluated. Methods Rats were intraperitoneally injected LPS (3 mg/kg)、Cur(300 mg/kg)and Cur(300 mg/kg)respectively or NS398 which was a selective inhibitor of COX-2 (10 mg/kg) conbined with LPS(3 mg/kg).The C-fiber evoked field potentials were recorded at the superficial layers of spinal dorsal horn in the lumbar enlargement to observe the effect of Curcumin and NS398,a selective inhibitor of COX-2, on the chronic exposure to LPS- induced inhibition of LTP after 7 days. The expression of cox-2 under different conditions was determined by Western blotting method. Results ①Spinal LTP was significantly attenuated by chronic exposure to LPS and this effect was robustly inhibited by Curcumin. But Curcumin itself did not affect spinal LTP by tetani. ②Chronic exposure to LPS-induced inhibition of spinal LTP was reversed by NS398. ③Elevated expression of COX-2 protein in LPS-treated rats at 7d after injection was significantly suppressed by NS398 and Cur respectively. Conclusion Chronic exposure to LPS-induced inhibition of spinal LTP are reversed by Curcumin and this effect may be mediated via COX-2.

Key words: Cyclooxygenase-2, Curcumin, Lipopolysaccharide, Long-term potentiation, Spinal cord

中图分类号: 

  • R338.2
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