中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2012, Vol. 41 ›› Issue (2): 108–114.

• 基础医学 • 上一篇    下一篇

两种不同纯化大鼠胰岛细胞方法的比较

李晓航, 张佳林, 李乐, 康铁利, 程颖, 石蕊, 赵宁, 刘永锋   

  1. 中国医科大学附属第一医院普通外科教研室肝胆外科暨器官移植科,沈阳 110001
  • 收稿日期:2012-09-20 修回日期:2012-09-20 出版日期:2012-02-20 发布日期:2012-09-21
  • 基金资助:
    国家自然科学基金资助项目(30371359;30870644);国家自然科学基金专项基金资助项目(31140059)

Comparison between tTwo Purification Methods for Purification of Rat Islets

LI Xiao-hang, ZHANG Jia-lin, LI Le, KANG Tie-li, CHENG Ying, SHI Rui, ZHAO Ning, LIU Yong-feng   

  1. Department of General Surgery, The First Hospital, China Medical University, Shenyang 110001, China
  • Received:2012-09-20 Revised:2012-09-20 Online:2012-02-20 Published:2012-09-21
  • Contact: 张佳林 E-mail:jlz2000@126.com

摘要: 目的 比较分析OptiPrep和Ficoll两种介质纯化大鼠胰岛细胞的效果。方法 将24只SD大鼠随机分成2组,采用明尼苏达大学改良方法提取大鼠胰岛细胞,消化分离胰岛细胞后,分别采用不连续密度梯度Ficoll离心法和OptiPrep离心法纯化胰岛,比较纯化后胰岛细胞数量、纯度、存活率及功能,观察OptiPrep和Ficoll两种介质对大鼠胰岛纯化效果的影响。结果 与 Ficoll组相比,OptiPrep组胰岛细胞的产量(分别为304±13.57和346±9.09)和纯度(分别为88.25%±2.22%和94%±0.82%)均显著增加(P0.05)。 结论 OptiPrep法纯化大鼠胰岛比传统的Ficoll法更有优势,而且OptiPrep价格相对便宜,可以作为纯化大鼠胰岛的常规方法。

关键词: 胰岛细胞, 纯化, 分离, 大鼠, 胰岛细胞, 纯化, 分离, 大鼠

Abstract: Objective To compare the effects of OptiPrep and Ficoll on the purification of rat islets. Methods Islets were isolated from the pancreata of 24 SD rats by means of modified Universtiy University of Minnesota’s method. After an successful enzymatic digestion, the digested tissue werewas randomly purified by Ficoll andor OptiPrep randomly. Compared the islet yields, purity, survival and function between the two groups after purification and the effects of two media on the purification of rat islets. Results Compared with the Ficoll group, yield and purity of islets were significantly higher in the OptiPrep group(304±13.57 vs 346±9.09;88.25% ± 2.22% vs 94% ± 0.82%, P 0.05). Conclusion The purification method usingby OptiPrep has some advantages compared withover the traditional purification method by using Ficoll, .therefore, OptiPrep with Owing to its cheap price , OptiPrep can be used as an conventional method of for purification of rat islets.

Key words: islets of langerhans, purification, isolation, rat, iIslets of langerhans, Ppurification, Iisolation, Rrat

中图分类号: 

  • R587.1
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