中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2012, Vol. 41 ›› Issue (2): 120–123.

• 基础医学 • 上一篇    下一篇

缺氧复氧对乳鼠心肌细胞的损伤及其时间变化的研究

侯志文, 于波, 孟凡吉, 孙景奕, 府晓丹   

  1. 中国医科大学 附属第一医院心内科,沈阳 110001
  • 收稿日期:2012-09-20 修回日期:2012-09-20 出版日期:2012-02-20 发布日期:2012-09-21
  • 基金资助:
    辽宁省教育厅高校科研计划项目(2008804),辽宁省科技计划项目(2007408001-3)

Study of Anoxia/Reoxygenation Injury in Rat Myocardial Cells and Various Times Injury of Reoxygenation

HOU Zhi-wen, YU Bo, MENG Fan-ji, SUN Jing-yi, FU Xiao-dan   

  1. Department of Cardiology ,The First Affiliated Hospital of China Medical University, Shenyang, 110001,China
  • Received:2012-09-20 Revised:2012-09-20 Online:2012-02-20 Published:2012-09-21
  • Contact: 于波 E-mail:ybdy@hotmail.com

摘要: 目的 观察乳鼠心肌细胞的缺氧损伤以及缺氧复氧条件下不同复氧时间对乳鼠心肌细胞损伤的变化。方法 用MTT法检测缺氧损伤及不同复氧时间对乳鼠心肌细胞存活率的影响;LDH、T-SOD/GSH/GSSG试剂盒检测缺氧损伤及不同复氧时间下细胞损伤程度。结果 MTT及LDH、T-SOD/GSH/GSSG可检测到缺氧损伤及复氧损伤,且损伤于复氧1 h后趋于稳定。结论 乳鼠心肌细胞缺氧复氧损伤于复氧1 h后趋于稳定。

关键词: 心肌细胞, 缺氧复氧, 损伤

Abstract: Objective To investigate the injury in rat myocardial cells under anoxia(A) and the change of neonatal rat myocardial cells injury under anoxia(A)/ reoxygenation(AR) at various times. Methods The effect on survival rate of different neonatal rat myocardial cells under A and A/R at various times was detected by MTT assay and the degree of cell injury under A and A/R at various times was detected by LDH, T-SOD, GSH/GSSG detection kit. Results Compared with the control group, the injury can be detected in A and A/R groups, and the injury stabilized at 1 hour after reoxygenation. Conclusion Neonatal rat myocardial cells injury under anoxia(A)/ reoxygenation(AR) stabilized at 1 hour after reoxygenation.

Key words: Myocardial cells, Anoxia/Reoxygenation, Injury

中图分类号: 

  • R3
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