中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2012, Vol. 41 ›› Issue (2): 128–130.

• 基础医学 • 上一篇    下一篇

Survivin基因真核表达载体的构建及鉴定

范红杰1、于维东2、何志义3   

  1. 中国医科大学1附属盛京医院神经内科 沈阳 110004;2附属第一医院干诊科;3附属第一医院神经内科,沈阳 110001
  • 收稿日期:2012-09-20 修回日期:2012-09-20 出版日期:2012-02-20 发布日期:2012-09-21

Construction and Identification of Survivin Gene Eukaryotic Expression Vector

FAN Hong-jie1,YU Wei-dong2,HE Zhi-yi3   

  1. 1.Department of Neurology, Shengjing Hospital,China Medical University, Shenyang 110004 ,China;2.Department of Gerontology, The First Hospital, China Medical University, Shenyang 110001, China; 3.Department of Neurology, The First Hospital, China Medical University, Shenyang 110001, China
  • Received:2012-09-20 Revised:2012-09-20 Online:2012-02-20 Published:2012-09-21

摘要: 目地 构建pcDNA3.1-Survivin基因的真核表达载体并鉴定。方法 提取大鼠肝细胞总RNA,RT-PCR 法扩增Survivin cDNA 片段, 双酶切构建pcDNA3.1-Survivin, 重组载体。结果 重组真核表达载体经PCR、酶切鉴定及测序证实正确。结论 成功构建了真核表达载体pcDNA3.1-Survivin。

关键词: 关键词:Survivin 基因,构建,鉴定, Survivin 基因, 构建, 鉴定

Abstract: Objective: To construct and identify the pcDNA3.1-survivin gene eukaryotic expression vector . Methods : Total RNA extracted from rat liver was used for amplification of survivin cDNA by RT-PCR and construction of recombinant pcDNA3.1-survivin plasmid vector by double digestion with restriction enzymes. Results:The recombinant eukaryotic expression vector was confirmed by PCR, enzyme digestion and sequencing methods. Conclusion:The pcDNA3.1-survivin eukaryotic expression vector has been successfully constructed.

Key words: Key words: Survivin gene, construction, identification

中图分类号: 

  • R737.33
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