中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2012, Vol. 41 ›› Issue (2): 143–145.

• 临床医学 • 上一篇    下一篇

HMGB1基因真核细胞表达载体的构建及其在人脐静脉血管内皮细胞中的表达

张晓娟, 李旭, 栾正刚, 马晓春   

  1. 中国医科大学附属第一医院重症医学科,沈阳 110001
  • 收稿日期:2012-09-20 修回日期:2012-09-20 出版日期:2012-02-20 发布日期:2012-09-21

Construction of a Recombinant Eukaryotic Expression Plasmid Containing Human HMGB1 Gene and its Expression in Human Umbilical Vein Endothelial Cells

ZHANG Xiao-Juan , LI Xu , LUAN Zheng-Gang, MA Xiao-Chun   

  1. Department of Intensive Care Unit, The First Affiliated Hospital of China Medical University, Shenyang 110001, China
  • Received:2012-09-20 Revised:2012-09-20 Online:2012-02-20 Published:2012-09-21

摘要: 目的 构建HMGB1基因真核细胞表达载体并检测其在真核细胞中的表达?方法 提取人脐静脉血管内皮细胞(HUVEC)mRNA,反转录为cDNA?PCR 扩增HMGB1全长编码基因,构建成pcDNA-3.1-myc-his-B-HMGB1真核细胞表达载体?转染HUVEC,Western blot 检测转染后HMGB1基因的表达改变?结果 构建HMGB1全长基因真核细胞表达载体成功,酶切鉴定及测序鉴定表明载体构建正确,转染HUVEC后可检测到外源性蛋白的表达,对照组无外源性蛋白表达?结论 成功构建了HMGB1全长基因真核细胞表达载体并可转染至HUVEC中

关键词: HMGB1基因, 人脐静脉血管内皮细胞, 真核细胞表达载体, 转染

Abstract: Objective To construct a recombinant eukaryotic expression plasmid containing human HMGB1 gene and express the gene in human umbilical vein endothelial cells (HUVECs).

Key words: High-mobility group box 1 (HMGB1), Human umbilical venular endothelial cells (HUVEC), Eukaryotic expression plasmid , Transfect

中图分类号: 

  • R364.5
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