中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2012, Vol. 41 ›› Issue (2): 148–150.

• 临床医学 • 上一篇    下一篇

神经母胞瘤恶性进展相关基因初步筛选及验证

于萌1, 朱育焱2, 杨春明2, 王海波2, 李元元3, 中川原章3, 郑志红1   

  1. 1. 中国医科大学实验动物部转基因实验室, 沈阳 110001,2. 中国医科大学附属第一医院泌尿外科, 沈阳 110001,3. 日本千叶癌中心研究局肿瘤基因组学研究室 日本千叶 2608718
  • 收稿日期:2012-09-20 修回日期:2012-09-20 出版日期:2012-02-20 发布日期:2012-09-21
  • 基金资助:
    国家自然科学基金资助项目(31000572),教育部新教师基金资助项目(20102104120013),辽宁省博士科研启动基金资助项目(20101148),辽宁省教育厅科学技术研究基金资助项目(L2010622)

Screening and Identification of Candidate Genes Associated with Progression of Neuroblastoma

YU Meng 1, ZHU Yu-yan 2, YANG Chun-ming 2, WANG Hai-bo 2, LI Yuan-yuan 3, NAKAGAWARA Akira 3, ZHENG Zhi-hong 1   

  1. 1. Department of Reproductive Biology and Transgenic Animals, China Medical University, Shenyang 110001, China; 2. Department of Urology,The First Hospital ,China Medical University, Shenyang 110001, China; 3. Laboratory of Cancer Genomics, Chiba Cancer Center Research Institute, Chiba 260-8717, Japan
  • Received:2012-09-20 Revised:2012-09-20 Online:2012-02-20 Published:2012-09-21

摘要: 目的 利用神经母细胞瘤(NB)临床标本筛选与恶性进展相关的基因。方法 利用比较基因组杂交技术和表达谱基因芯片技术筛选人类17号染色体上某些区域可能存在的与NB密切相关的候选基因,并结合生物信息学分析和RT-PCR检测结果对候选基因进行进一步鉴定。结果 通过初步筛选,在人类17号染色体增添区域发现了与NB恶性进展相关的新基因(ncRAN),该基因包含2个剪切体,Nbla10727和Nbla12061。ncRAN在各组织中及MYCN单/多拷贝组细胞中呈现差异性表达,其高表达与不良预后密切相关(P=0.000221)。结论 在17号染色体增添区所鉴定的新基因很可能是一个与NB恶性进展密切相关的癌基因。

关键词: 神经母细胞瘤, 17号染色体, 癌基因, cDNA 芯片, 神经母细胞瘤, 17号染色体, 癌基因, cDNA 芯片

Abstract: Objective To discover the molecular mechanisms underlying progression of human neuroblastoma by screening the candidate genes in clinical specimens. Methods The candidate genes mapped on human chromosome 17q gain, which was significantly associated with the neuroblastoma, were screened by combining the array-CGH results with expression profiling, and identified by bioinformatic analysis and RT-PCR. Results A novel gene linked to progression of neuroblastoma named ncRAN was found on chromosome 17q gain, which included two transcripts, Nbla10727 and Nbla12061, and differential expression can be detected in various tissues and neuroblastoma cell lines with a single copy and amplification of MYCN. Furthermore, high expression of ncRAN was associated with poor prognosis (P=0.000221). Conclusion The novel gene mapped on chromosome 17q gain may be an oncogene which is associated with progression of neuroblastoma.

Key words: neuroblastoma, chromosome 17, oncogene, cDNA chip, Neuroblastoma, Chromosome 17, Oncogene, cDNA chip

[1] Brodeur GM. Neuroblastoma: biological insights into a clinical enigma [J]. Nature Rev Cancer, 2003, 3 (3): 203-216.B.
[2] Riley RD, Heney D, Jones DR, et al. A systematic review of molecular and biological tumor markers in neuroblastoma [J]. Clin Cancer Res, 2004, 10 (1 Pt 1): 4-12.
[3] Attiyeh EF, London WB, Mosse YP, et al. Chromosome 1p and 11q deletions and outcome in neuroblastoma [J]. N Engl J Med, 2005, 353 (21): 2243-2253.
[4] Morowitz M, Shusterman S, Mosse Y, et al. Detection of single-copy chromosome 17q gain in human neuroblastomas using real-time quantitative polymerase chain reaction [J]. Mod Pathol, 2003, 16(12):1248-1256.
[5] Combaret V, Br閖on S, Iacono I, et al. Determination of 17q gain in patients with neuroblastoma by analysis of circulating DNA [J]. Pediatr Blood Cancer, 2011, 56 (5): 757-761.
[6] Ohira M, Morohashi A, Inuzuka H, et al. Expression profiling and characterization of 4200 genes cloned from primary neuroblastomas: identification of 305 genes differentially expressed between favorable and unfavorable subsets [J]. Oncogene, 2003, 22 (35): 5525-5536.
[7] Islam A, Kageyama H, Takada N, et al. High expression of Survivin, mapped to 17q25, is significantly associated with poor prognostic factors and promotes cell survival in human neuroblastoma [J]. Oncogene, 2000, 19 (5): 617-623.
[1] 周景儒, 赵婉妮, 梁剑峰. 榄香烯对神经母细胞瘤增殖的抑制作用[J]. 中国医科大学学报, 2017, 46(12): 1062-1066.
[2] 阎敬,关一夫. 原癌基因MET启动子的G-四链体结构[J]. 中国医科大学学报, 2016, 45(5): 402-405.
[3] 宋耀文,韩冲,李光,党军,于洋洋,姚雷,张硕. LKB1蛋白表达与非小细胞肺癌放射敏感性的关系[J]. 中国医科大学学报, 2013, 42(8): 706-709.
[4] 宋园园,李红. 姜黄素下调Notch1抑制人结肠腺癌细胞系SW480的增殖活性[J]. 中国医科大学学报, 2013, 42(6): 521-525.
[5] 郝祥俊,于晓敏,宋成军,王小杰,陈志宏. 丝胶对糖尿病大鼠肾脏ERK信号通路的影响[J]. 中国医科大学学报, 2013, 42(1): 60-64.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!

中国医科大学学报版权所有©2018

未经允许,严禁擅自转载本站图文资料

地址:中国 沈阳市沈北新区蒲河路77号 110122

辽ICP备05014850

JOURNAL OF CHINA MEDICAL UNIVERSITY

ADDRESS: NO.77 PUHE ROAD

SHENYANG NORTH NEW AREA, SHENYANG

LIAONING PROVINCE, P.R. CHINA