中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2012, Vol. 41 ›› Issue (2): 175–177.

• 临床医学 • 上一篇    下一篇

Twist介导的上皮间质转化在卵巢癌组织中的作用

王文双1, 杨兴升2, 马先莹1,   

  1. 1.山东大学齐鲁医院妇科,济南250012;2.山东烟台毓璜顶医院妇科,山东烟台 264000
  • 收稿日期:2012-09-20 修回日期:2012-09-20 出版日期:2012-02-20 发布日期:2012-09-21

Effect of Twist on Epithelial-Mesenchymal Transitions in Ovarian Cancer Tissue

WANG Wen-shuang1, YANG Xing-sheng2,MA Xian-Ying1   

  1. 1. Department of Gynecology, Qilu Hospital Of Shandong University ,Jinan 250012,China;2.Department of Gynecology,Yantai Yuhuangding Hospital,Yantai 264000,China
  • Received:2012-09-20 Revised:2012-09-20 Online:2012-02-20 Published:2012-09-21
  • Contact: 杨兴升 E-mail:xingshengyang@yahoo.com

摘要: 目的 检测卵巢癌组织中Twist、E-cadherin及N-cadherin蛋白和mRNA的表达,探讨Twist介导的上皮间质转化(EMT)现象在卵巢癌发生、发展及转移中的作用。方法 分别采用免疫组化方法和RT-PCR法检测42例卵巢癌组织中介导EMT发生的转录因子Twist、E-cadherin及N-cadherin蛋白及其mRNA的表达,30例卵巢良性肿瘤组织作为对照。结果 卵巢癌组织中Twist、E-cadherin及N-cadherin蛋白阳性表达率分别为66.7%、28.6%和47.6%,卵巢良性肿瘤组织分别为26.7%、90.0%和13.3%,;Twist、E-cadherin及N-cadherin mRNA在卵巢癌组织中的表达量分别为(1.49±0.53)、(0.82±0.24)、(1.55±0.56),卵巢良性肿瘤组织中分别为(1.18±0.34)、(1.09±0.20)、(1.18±0.35)。两种组织各项指标比较差异有统计学意义(P<0.05)。Twist、E-cadherin及N-cadherin的表达与卵巢癌临床分期、分化程度及淋巴结转移有关(P <0.05)。结论 在卵巢癌组织中,Twist、N-cadherin表达上调,E-cadherin表达下调,Twist介导的EMT可能与卵巢癌的侵袭转移有关。

关键词: Twist, E-cadherin, N-cadherin, 卵巢癌, 上皮间质转化

Abstract: Objective To observe the expression of Twist, E-cadherin and N-cadherin in ovarian cancer tissue and investigate the role of epithelial-mesenchymal transitions (EMT) in metastasis of ovarian cancer. Methods The expression of Twist, E-cadherin and N-cadherin proteins and their mRNA in 42 ovarian cancer cases was detected by immunohistochemistry and RT-PCR, and 30 cases of ovarian benign tumor were selected as control group. Results The positive expression rates of Twist, E-cadherin and N-cadherin proteins in ovarian cancer tissue were 66.7%、28.6% and 47.6% respectively,whereas in the control group the positive expression rates were 26.7%、90.0% and 13.3%; The expression levels of Twist, E-cadherin and N-cadherin mRNA were 1.49±0.53、0.82±0.24 and 1.55±0.56,whereas in the control group the expression levels were 1.18±0.34、1.09±0.20 and 1.18±0.35. There was statistical difference between the two groups (P<0.05). Conclusion The expression levels of Twist and N-cadherin are upregulated, and the expression level of E-cadherin is downregulated. The role of Twist in EMT-mediated invasiveness may be related to metastasis and progression of ovarian cancer.

Key words: Twist, E-cadherin, N-cadherin, Ovarian cancer, Epithelial-mesenchymal transitions

中图分类号: 

  • R737.31
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