中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2012, Vol. 41 ›› Issue (4): 303–306.

• 基础医学 • 上一篇    下一篇

普伐他汀对2型糖尿病大鼠视网膜组织中转化生长因子β1表达的影响

李婕,杨宁,王思敏,林婷婷,宁宏   

  1. 中国医科大学附属第一医院眼科,沈阳110001
  • 收稿日期:2012-09-24 修回日期:2012-09-24 出版日期:2012-04-20 发布日期:2012-09-26

Effects of Pravastatin on the eExpression of TGF-β1 in Retina of Type Ⅱ Diabetes Rat Model s

LI Jie,YANG Ning,WANG Si-min,LIN Ting-ting,NING Hong   

  1. (Department of Ophthalmology, The First Hospital, China Medical University, Shenyang 110001, China)
  • Received:2012-09-24 Revised:2012-09-24 Online:2012-04-20 Published:2012-09-26

摘要: 目的 了解普伐他汀对2型糖尿病大鼠视网膜组织中转化生长因子β1(TGF-β1)表达的影响,探讨普伐他汀对糖尿病性视网膜病变的防治作用。方法 将54只Wistar大鼠随机分为正常组、糖尿病组和普伐他汀治疗组,每组18只。用链脲佐菌素诱导糖尿病组和普伐他汀治疗组大鼠建立2型糖尿病模型,正常组和糖尿病组正常饮食,普伐他汀治疗组在正常饮食中加入普伐他汀(100 mg?kg﹣1?d﹣1)。30周后取各组大鼠视网膜,分别用免疫组化法和Real-time PCR法检测视网膜组织中TGF-β1的表达水平。结果 TGF-β1阳性表达于视网膜神经节细胞胞质中。糖尿病组视网膜组织变薄,神经节细胞数目减少;与正常组相比,普伐他汀治疗组视网膜形态无明显改变,神经节细胞数目无明显减少。糖尿病组视网膜组织中TGF-β1表达明显高于正常组(P0.05),普伐他汀治疗组明显低于糖尿病组(P0.05)。结论 普伐他汀能够阻止或延缓糖尿病视网膜形态改变,能够降低糖尿病大鼠视网膜组织中TGF-β1的表达水平。

关键词: 关键词 普伐他汀, TGF-β1, 糖尿病视网膜病变

Abstract: Abstract Objective To investigate the impacts effects of pravastatin on the expression of TGF-β1 in retina of Ttype Ⅱ Ddiabetes Rrats Model, and the preventive effect of pravastatin in the pathological changes in diabetic retina. Methods 54 Fifty-four Wistar rats, 180-200g, were randomly divided into three groups normal group, diabetic group and pravastatin treatment group, with 18 rats in each. Rats in diabetic group and pravastatin treatment group were induced with streptozotocin to Ttype Ⅱ Ddiabetes Rrat Mmodel. The rats in the first two groups were fed with normal diet and the rats in the pravastatin treatment group were fed with normal diet plus pravastatin in 100 mgkgday. After 30 weeks, rats in all groups were sacrificed, respectively. detected the The expression of TGF-β1 in retinal tissue was detected with immunohistochemistry and Real-time PCR. Results Positive expression of TGF-β1 was in cytoplasm of Retina ganglion cells. In the diabetic group compared Compared with the normal group, the thickness of retina became thinning and the number of ganglion cells decreased in the diabetic group, while those . That did notwere not significantly changed in the pravastatin treatment group. The expression of TGF-β1 in diabetic group was significantly higher than that in the normal group (P0.05), and the expression of TGF-β1 in pravastatin treatment group was significantly lower than that in diabetes group (P0.05). Conclusion Pravastatin could control and delay the morphological changes of diabetic retinopathy, and pravastatin could decrease the expression of TGF-β1 in diabetic retina.

Key words: pravastatin, TGF-β1, diabetic retinopathy

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  • R774.1 文献标志码 A 文章编号
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