中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2012, Vol. 41 ›› Issue (5): 388–390.

• 基础医学 •    下一篇

AKAP7γ在小鼠卵母细胞减数分裂过程中的表达

王述森,罗阳,张阳   

  1. 中国医科大学基础医学院医学基因组学教研室,教育部医学细胞生物学重点实验室,沈阳110001
  • 收稿日期:2012-09-27 修回日期:2012-09-27 出版日期:2012-05-20 发布日期:2012-09-27

Expression and Modification of AKAP7γ during Meiosis in Mouse Oocytes

WANG Shu-sen,LUO Yang,ZHANG Yang   

  1. The Research Center for Medical Genomics, Key Laboratory of Medical Cell Biology, Ministry of Education, College of Basic Medical Science, China Medical University, Shenyang 110001, China
  • Received:2012-09-27 Revised:2012-09-27 Online:2012-05-20 Published:2012-09-27

摘要: 目的 研究小鼠卵母细胞减数分裂过程中AKAP7γ的表达情况。 方法 收集生发泡(GV)期及第2次减数分裂中期(MII期)小鼠卵母细胞,Western blot方法检测AKAP7γ 蛋白的表达及修饰情况。 结果 在小鼠卵母细胞减数分裂过程中,AKAP7γ在GV及MII期均有表达,且在MII期卵母细胞中,AKAP7γ电泳迁移率变慢,应用冈田酸及碱性磷酸酶(ALP)分别处理GV 及MII 期卵母细胞,AKAP7γ电泳迁移率差异消失。结论 随减数分裂进程,在MII期小鼠卵母细胞中,AKAP7γ发生磷酸化修饰,提示AKAP7γ不仅作为A型激酶锚定蛋白发挥支架蛋白的作用,其本身也可作为其他激酶的底物和效应分子。

关键词: 卵母细胞, AKAP7γ, 磷酸化

Abstract: Objective In the mammalian oocytes, protein kinase A (PKA) has critical functions in the maintenance of meiotic arrest and oocyte maturation. Spatial regulation of PKA is accomplished by its sequestration via A-kinase anchor proteins (AKAPs). In this study we aimed to examine the endogenous expression of AKAP7γ in the Germinal Vesicle (GV) and MII stage of mouse oocytes. Methods The GV and MII stage of mouse oocytes for the presence and modification of the AKAP7γ were examined by western blot. Results AKAP7γ exhibited a retarded electrophoretic motility at MII of meiosis compared with GV stage in mouse oocytes. The motility shift of AKAP7γ was susceptible to okadaic acid (OA) and alkaline phosphatase (ALP). Conclusion Mouse oocytes at MII of meiosis express a phosphorylated form of AKAP7γ. AKAP7γ might function in mouse oocytes not only as an anchoring protein but also as a substrate and effector for the anchored kinases.

Key words: oocyte, AKAP7γ, phosphorylation

中图分类号: 

  • Q291
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