中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2012, Vol. 41 ›› Issue (6): 497–500.

• 基础医学 • 上一篇    下一篇

EB病毒核抗原3C提高Gemin3基因的表达

郭毅1,孔繁明2 ,赵楠1   

  1. (1.中国医科大学附属第一医院妇科,沈阳 110001;2. 辽宁省宽甸县中心医院妇产科, 辽宁 宽甸 118200)
  • 收稿日期:2012-09-25 修回日期:2012-09-25 出版日期:2012-06-20 发布日期:2012-09-27

EBNA3C Up-regulates Gemin3 Gene Expression

GUO Yi1,KONG Fan-ming2,ZHAO Nan1   

  1. (1. Department of Gynecology, The First Hospital, China Medical University, Shengyang 110001, China; 2. Department of Obstetrics and Gynecology, Central Hospital of Kuandian County, Quandian 118200, China)
  • Received:2012-09-25 Revised:2012-09-25 Online:2012-06-20 Published:2012-09-27

摘要: 目的 探讨EB病毒核抗原(EBNA)3C对Gemin3基因表达的影响。方法 共转染EBNA3C和Gemin3基因至HEK293细胞。依靠慢病毒载体介导发卡RNA干涉敲减EBNA3C基因的表达,并经嘌呤霉素筛选获得稳定的EBNA3C低表达细胞系,采用Western blot检测EBNA3C对Gemin3蛋白表达的影响。结果 Gemin3基因的表达随着EBNA3C表达量的增加而增加,呈剂量依赖性,在EBNA3C基因敲减细胞中Gemin3的表达减少。结论EBNA3C可提高Gemin3基因表达水平。

关键词: EB病毒核抗原3C, Gemin3, 基因表达调控, 基因敲减

Abstract: Objective To investigate the effect of EBNA3C on Gemin3 expression. Methods Plasmids of EBNA3C and Gemin3 were co-transfected into HEK 293 cells; EBNA3C knock-down stable cell lines were madeknocked down by lentivirus-delivered small hairpin RNA and then underwent puromycin selection to obtain EBNA3C low-express cell lines. Western blot was used to check tThe effect of EBNA3C on Gemin3 expression was checked by Western blot. Results Gemin3 gene expression increased as with EBNA3C transfected transfection in a dose dependent manner, Gemin3 gene expression wasand decreased in the EBNA3C knock-down cell lines. Conclusion EBNA3C can upregulates Gemin3 gene expression level.

Key words: EBNA3C, Gemin3, gene expression regulation, gene knock-down

中图分类号: 

  • 中图分类号 R466.5 文献标识码 A 文章编号
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