中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2012, Vol. 41 ›› Issue (6): 514–516.

• 基础医学 • 上一篇    下一篇

β-catenin评价肝细胞癌125I粒子植入治疗的实验研究

谢锋1,王晓明1,卢再鸣1,刘峥嵘2   

  1. (1.中国医科大学附属盛京医院放射科,沈阳 110004;2.辽宁省人民医院普外科,沈阳 110016)
  • 收稿日期:2012-09-25 修回日期:2012-09-25 出版日期:2012-06-20 发布日期:2012-09-27
  • 基金资助:
    辽宁省医学高峰建设工程项目(辽卫函字2010-696);盛京自由研究者计划基金资助项目(2008-32)。

Evaluation of 125I Seed Implantation for Hepatocellular Carcinoma Therapy by β-catenin

XIE Feng1, WANG Xiao-ming1, LU Zai-ming1 ,LIU Zheng-rong 2   

  1. (1.Department of Radiology, Shengjing Hospital, China Medical University, Shenyang 110004,China;2.Department of General Surgery,The People’s Hospital of Liaoning Province,Shenyang 110016,China)
  • Received:2012-09-25 Revised:2012-09-25 Online:2012-06-20 Published:2012-09-27

摘要: 目的 研究β-catenin在HepG2肝癌细胞株中的表达以及125I粒子对其表达的影响,以β-catenin为检测指标,评价肝细胞癌125I粒子植入治疗的疗效。方法 采用免疫荧光法、Western blot和real-time PCR的方法观察β-catenin在125I粒子照射1,2,4周各组HepG2肝癌细胞株中的表达。比较各实验组和对照组β-catenin在HepG2肝癌细胞株中表达。结果 免疫荧光实验显示照射后β-catenin在肝癌细胞中的表达与照射前比较明显降低,Western-blot实验显示125I粒子照射后β-catenin蛋白的表达较照射前明显降低(P=0.009),并且与照射时间呈正相关(P=0.041)。real-time PCR实验显示125I粒子照射后,肝癌细胞β-catenin RNA明显下降(P=0.000),并且下降程度也同125I粒子照射时间呈正相关(P=0.009)。结论 在HepG2肝癌细胞株中存在β-catenin的高表达。125I粒子近距离照射后,β-catenin在HepG2肝癌细胞株中的表达呈下降趋势,并且下降程度与125I粒子照射时间呈正相关。125I粒子近距离照射对于HepG2肝癌细胞株具有疗效。

关键词: β-catenin, 肝癌, 125I粒子, 内照射治疗, Western-blot, real-time PCR

Abstract: Objective To investigate β-catenin expression in HepG2 liver cancer cells as well as the effects of 125I seed on it, so as to evaluate the 125I seed implantation efficacy taking β-catenin as the testing indice. Methods Immunofluorescence, western-blot and real-time PCR were used to observe the β-catenin expression in HepG2 liver cancer cells in radiation sessions with 125I seed of one week, two weeks and four weeks, respectively. Then β-catenin expressions in HepG2 liver cancer cells in experimental and control groups were compared. Results Immunofluorescence assay showed that β-catenin expression in hepatoma cells was significantly reduced after irradiation. Western-blot experiments showed that 125I seed-irradiated β-catenin protein expression was significantly lower than that before irradiation(P = 0.009). It was positively correlated with the exposure time(P=0.041). Real-time PCR experiments showed that after 125I seed irradiation, β-catenin RNA significantly decreased in the liver cancer cells(P = 0.000), and also proved that it was positively correlated with the exposure time(P=0.009). Conclusion HepG2 liver cancer cell lines showed high β-catenin expression. After 125I seed brachytherapy, β-catenin in HepG2 liver cancer cell lines decreased. The degree of decrease in β-catenin expression was positively correlated with 125I seed irradiation time. 125I seed brachytherapy for the HepG2 hepatoma cell line was effective.

Key words: β-catenin, hepatic carcinoma, 125I seed, internal radiotherapy, western-blot, real-time PCR

中图分类号: 

  • 中图分类号 R815.2 文献标志码 A 文章编号
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