中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2012, Vol. 41 ›› Issue (6): 524–526.

• 口腔医学 • 上一篇    下一篇

TNF-α刺激人牙周膜细胞表达MMP-9的研究

赵戬,潘春玲,刘俊超,李倩,潘亚萍   

  1. (中国医科大学口腔医学院牙周科 辽宁省口腔医学研究所牙周病学研究室 沈阳 110002)
  • 收稿日期:2012-09-25 修回日期:2012-09-25 出版日期:2012-06-20 发布日期:2012-09-27

The eEffects of TNF-α on MMP-9 production by Expression in Hhuman Pperiodontal Lligment Ccells

ZHAO Jian, PAN Chun-ling, LIU Jun-chao, LI Qian, PAN Ya-ping   

  1. (Department of Periodontology, School of Stomatology, China Medical University, Shenyang . 110002, China)
  • Received:2012-09-25 Revised:2012-09-25 Online:2012-06-20 Published:2012-09-27

摘要: 目的 研究TNF-α对hPDLCs表达MMP-9的影响。方法 分别以1、2、5ng/mL浓度的TNF-α刺激hPDLCs 24h,以及2ng/mL浓度的TNF-α刺激hPDLCs 6h、12h、24h、48h,应用ELISA法测定细胞上清液中MMP-9的表达水平。结果 随着TNF-α浓度增大和作用时间延长,hPDLCs表达MMP-9均增加。结论 TNF-α能够以时间和剂量依赖的方式增强hPDLCs表达MMP-9。

关键词: 人牙周膜细胞, 基质金属蛋白酶, 细胞因子

Abstract: Objective To examine the effects of tumor necrosis factor (TNF-α) on the regulation of MMP-9 expression in human periodontal ligment cells (hPDLCs). Method hPDLCs were treated with increasing different concentrations (1、, 2、, 5ng/ml) of recombinant human TNF-α (Boster, Wuhan) for 24 h, or and were stimulated by 2ng/ml TNF-α for 6h, 12h, 24h and 48h, respectively. MMP-9 protein levels in cultured supernatants were determined by enzyme-linked immunosorbent assay. Results Compared with the control group, Aa significant increase in MMP-9 expression was seen detected after TNF-α stimulation compared to the control group with with the increase of the increasing action time and concentration. Conclusions TNF-α is a potent inducer of MMP-9 secretion in both a dose- and time-dependent manner.

Key words: human periodontal ligment cells,, matrix metalloproteinase,, cytokines

中图分类号: 

  • 中图分类号R781.4 文献标志码 A 文章编号 0258-4646(2012)6
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