中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2013, Vol. 42 ›› Issue (10): 873–877.

• 论著 • 上一篇    下一篇

Genistein对乳腺癌MDA-MB-231细胞增殖、侵袭和迁移能力的影响

范盼红,赵文月,邹佳芮,马威,张晴晴,李连宏   

  1. 大连医科大学辽宁省肿瘤干细胞重点实验室,辽宁大连116044
  • 收稿日期:2013-06-26 修回日期:2013-11-26 出版日期:2013-10-18 发布日期:2013-11-26
  • 作者简介:范盼红(1987-),女,硕士研究生.

Effects of Genistein on Cell Proliferation, Invasion and Migration of MDA-MB-231 Breast Cancer Cells

FAN Pan-hong, ZHAO Wen-yue, ZOU Jia-rui, MA Wei, ZHANG Qing-qing, LI Lian-hong   

  1. The Center of Clinical Pathology of Dalian Medical Uiversity, Dalian 116044, China
  • Received:2013-06-26 Revised:2013-11-26 Online:2013-10-18 Published:2013-11-26

摘要: 目的 探讨Genistein对于高侵袭性乳腺癌MDA-MB-231细胞的增殖、侵袭和转移能力的影响。方法 采用CCK-8实验和流式细胞技术研究Genistein对乳腺癌MDA-MB-231细胞增殖的影响;细胞侵袭和迁移实验结合Westernblot检测Genistein作用前后细胞内基质金属蛋白酶2(MMP-2)和血管内皮生长因子(VEGF)蛋白的变化,研究其对乳腺癌细胞侵袭和迁移能力的影响及可能的作用机制。结果 CCK-8实验结果显示:Genistein处理乳腺癌MDA-MB-231细胞24h,可明显抑制癌细胞增殖,IC50为18.7#x003bc;mol/L。流式细胞技术检测细胞周期显示:经相同处理的乳腺癌MDA-MB-231细胞,处于S期的比例显著下降。细胞侵袭实验和迁移实验结果显示:随着Genistein浓度的增加,侵袭和迁移的细胞数量逐渐减少,差异有统计学意义(P<0.05)。Westernblot结果显示:Genistein处理后可降低MMP-2和VEGF蛋白的表达。结论 Genistein可抑制乳腺癌MDA-MB-231细胞增殖,降低其侵袭和迁移能力,其可能的机制是降低乳腺癌MDA-MB-231细胞MMP-2和VEGF的表达。

关键词: Genistein, 乳腺癌MDA-MB-231细胞, 基质金属蛋白酶2, 血管内皮生长因子

Abstract: Objective To investigate the effects of genistein on proliferation, invasion and metastasis of MDA-MB-231 breast cancer cells. Methods The proliferation of MDA-MB-231 breast cancer cells was measured by CCK-8 assay and flow cytometry assay. Transwell assay and Western Blot assay were used to evaluate the migration and invasion ability of MDA-MB-231 breast cancer cells. Results 24h after genistein treatment, CCK-8 assay showed that the proliferation of breast cancer cells was inhibited With an IC50 value of 18.7#x003bc;M. Cell cycle analysis by flow cytometry assay confirmed that the proportion of cells in S phase decreased significantly after genistein treatment (24h). The number of migrating cells in Transwell assay was sharply reduced . The invasion of MDA-MB-231 cells was inhibited compared with the blank control group (P<0.05). Western Blot assay revealed that MMP-2 and VEGF decreased significantly by 10 and 20#x003bc;M genistein. Conclusions Genistein could effectively inhibit the growth and invasion ability of MDA-MB-231 breast cancer cells in a dose dependent pattern in vitro, and the reduction of MMP-2 and VEGF expression may play an important role in this process.

Key words: Genistein, MDA-MB-231 breast cancer cells, MMP-2, VEGF

中图分类号: 

  • R737.9
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