中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2013, Vol. 42 ›› Issue (11): 978–981.

• 论著 • 上一篇    下一篇

2939例临床患者CD4+CD8+T淋巴细胞检测结果的回顾性分析

唐甜,张子宁,傅雅静,王亚男,姜拥军,耿文清,郭晓临,尚红   

  1. 中国医科大学附属第一医院检验科,卫生部艾滋病免疫学重点实验室,沈阳110001
  • 出版日期:2013-11-30 发布日期:2013-11-28
  • 作者简介:唐甜(1986-),女,技师.
  • 基金资助:
    国家科技重大专项课题资助项目(2012ZX10001-006)

Retrospective Analysis on CD4+CD8+ T Lymphocytes Percentages in 2 939 Clinical Cases

TANG Tian,ZHANG Zi-ning,FU Ya-jing,WANG Ya-nan,JIANG Yong-jun,GENG Wen-qing   

  1. Guo Xiao-Lin,SHANG HongKey Laboratory of AIDS Immunology of Ministry of Health,Department of Laboratory Medicine,The First Hospital,China Medical University,Shenyang 110001,China
  • Online:2013-11-30 Published:2013-11-28

摘要: 目的回顾分析中国医科大学附属第一医院2939例临床患者外周血CD4+CD8+双阳性(DP)T细胞在不同疾病和年龄中异常的比例,初步探讨这群细胞对疾病辅助诊断的意义。方法应用流式细胞术检测并比较临床患者CD4+CD8+DPT细胞亚群百分含量的差异,CD4+CD8+DPT细胞百分含量>5%判定为异常。结果患呼吸、风湿免疫、肿瘤、感染、肾脏、皮肤等系统疾病的患者CD4+CD8+DPT细胞异常的比例高于健康对照;这些CD4+CD8+DPT细胞异常的患者中按年龄分组,高年龄组(>65岁)CD4+CD8+DPT细胞异常的比例显著高于低年龄组(#x02264;65岁)(P<0.001),而健康对照CD4+CD8+DPT细胞异常的人群中,不同年龄组无统计学差异(P>0.05)。结论呼吸、风湿免疫、肿瘤、感染、肾脏、皮肤等系统疾病中CD4+CD8+DPT细胞异常的比例增加,且这些疾病的患者随着年龄的升高CD4+CD8+DPT细胞异常的比例亦增加(尤其患呼吸系统疾病和肿瘤的患者)。

关键词: T淋巴细胞亚群, CD4CD8T细胞, 疾病, 年龄

Abstract: Objective To retrospectively analyze 2939 patients admitted in the First Affiliated Hospital of China Medical University about the increased proportion of CD4+CD8+(double positive,DP)T cell among different diseases and ages,so as preliminarily explore the significance of this sub-population on assisting disease diagnosis. Methods The percentage of CD4+CD8+ T cell was determined by flow cytometer. The proportion of CD4+CD8+ T cell more than 5% was considered to be abnormal. Results The abnormal proportion of CD4+CD8+ T cell was significantly higher in patients with respiratory diseases,rheumatic diseases,tumor,infectious diseases,kidney diseases and dermatosis than healthy controls. In clinical patients,the abnormal proportion of CD4+CD8+ T cell was observed in older patients (>65) compared with younger patients (#x02264;65)(P<0.001). There was no significant difference in the abnormal proportion of CD4+CD8+ T cell in healthy controls with different ages (P>0.05). Conclusion The abnormal proportion of CD4+CD8+ T cell was significantly higher in respiratory diseases,rheumatic diseases,tumor,infectious diseases,kidney diseases and dermatosis. The proportion of CD4+CD8+ T cell was increased with the growth of age in clinical patients,in particular,who were suffering from respiratory diseases and cancer.

Key words: T lymphocytes subsets, CD4CD8T cell, disease, ages

[1] Blue ML, Daley JF, Levine H, et al. Coexpression of T4 and T8 on peripheral blood T cells demonstrated by two-color fluorescence flow cytometry[J]. J Immunol, 1985,134(4):2281-2286.
[2] Hernandez J, Garfias Y, Nieto A, et al. Comparative evaluation of the CD4+CD8+ and CD4+CD8- lymphocytes in the immune response to porcine rub#x003bc;lavirus[J]. Vet Immunol Immunopathol, 2001,79(3-4):249-259.
[3] Nam K, Akari H, Terao K, et al. Peripheral blood extrathymic CD4(+)CD8(+) T cells with high cytotoxic activity are from the same lineage as CD4(+)CD8(-) T cells in cynomolgus monkeys[J]. Int Immunol, 2000,12(7):1095-1103.
[4] 张宏伟,吴昊, 外周血CD4+CD8+ T细胞研究进展[J]. 北京医学, 2006,28(2):108-110.
[5] Suni MA, Ghanekar SA, Houck DW, et al. CD4(+)CD8(dim) T lymphocytes exhibit enhanced cytokine expression, proliferation and cytotoxic activity in response to HCMV and HIV-1 antigens[J]. Eur J Immunol, 2001,31(8):2512-2520.
[6] Munschauer FE, Stewart C, Jacobs L, et al. Circ#x003bc;lating CD3+ CD4+ CD8+ T lymphocytes in m#x003bc;ltiple sclerosis[J]. J Clin Immunol, 1993,13(2):113-118.
[7] Bang K, Lund M, Wu K, et al. CD4+ CD8+ (thymocyte-like) T lymphocytes present in blood and skin from patients with atopic dermatitis suggest immune dysreg#x003bc;lation[J]. Br J Dermatol, 2001,144(6):1140-1147.
[8] Chauhan NK, Vajpayee M, Mojumdar K, et al. Study of CD4+CD8+ double positive T-lymphocyte phenotype and function in Indian patients infected with HIV-1[J]. J Med Virol, 2012,84(6):845-856.
[9] Howe R, Dillon S, Rogers L, et al. Phenotypic and functional characterization of HIV-1-specific CD4+CD8+ double-positive T cells in early and chronic HIV-1 infection[J]. J Acquir Immune Defic Syndr, 2009,50(5):444-456.
[10] Ortolan, C, Forti E, Radin E, et al. Cytofluorimetric identification of two pop#x003bc;lations of double positive (CD4+,CD8+) T lymphocytes in human peripheral blood[J]. Biochem Biophys Res Commun, 1993,191(2):601-609.
[11] Freeman LM, Lam A, Petcu E, et al. Myeloma-induced alloreactive T cells arising in myeloma-infiltrated bones include double-positive CD8+CD4+ T cells: evidence from myeloma-bearing mouse model[J]. J Immunol, 2011,187(8):3987-3996.
[12] Desfrancois J, Derre L, Corvaisier M, et al. Increased frequency of nonconventional double positive CD4CD8 alphabeta T cells in human breast pleural effusions[J]. Int J Cancer, 2009,125(2):374-380.
[13] Rahemt#x003bc;llah A, Reichard KK, Preffer FI, et al. A double-positive CD4+CD8+ T-cell pop#x003bc;lation is commonly found in nod#x003bc;lar lymphocyte predominant Hodgkin lymphoma[J]. Am J Clin Pathol, 2006,126(5):805-814.
[14] 胡莉娜,吴丽娟,古宇等, 呼吸系统感染性疾病流式淋巴细胞亚群检测诊断效率的回顾性调查[J]. 国际检验医学杂志, 2011,32(20):2308-2312.
[15] Pescovitz MD, Sakopoulos AG, Gaddy JA, et al. Porcine peripheral blood CD4+/CD8+ dual expressing T-cells[J]. Vet Immunol Immunopathol, 1994,43(1-3):53-62.
[16] Lee WW, Nam KH, Terao K, et al. Age-related increase of peripheral CD4+ CD8+ double-positive T lymphocytes in cynomolgus monkeys: longitudinal study in relation to thymic involution[J]. Immunology, 2003,109(2):217-225.
[17] Ishimoto Y, Tomiyama-Miyaji C, Watanabe H, et al. Age-dependent variation in the proportion and number of intestinal lymphocyte subsets, especially natural killer T cells, double-positive CD4+ CD8+ cells and B220+ T cells, in mice[J]. Immunology, 2004,113(3):371-377.
[18] Ghia P, Prato G, Stella S, et al. Age-dependent accum#x003bc;lation of monoclonal CD4+CD8+ double positive T lymphocytes in the peripheral blood of the elderly[J]. Br J Haematol, 2007,139(5):780-790.
[1] 盖晴, 冷昶木, 丛树艳. 颅神经受累的吉兰-巴雷谱系疾病35例临床分析[J]. 中国医科大学学报, 2018, 47(9): 769-772.
[2] 吴枫, 孔令韬, 孙嘉泽, 汤艳清. 不同年龄发病的重性抑郁障碍患者髓鞘相关糖蛋白表达水平的差异[J]. 中国医科大学学报, 2018, 47(8): 705-707,712.
[3] 李晓曦, 刘文静, 姜文娟, 何志义. 以孤立性眩晕起病的老年脑梗死的临床分析[J]. 中国医科大学学报, 2018, 47(8): 744-747.
[4] 朱春明, 张鹤, 刘红波, 郑锐. 慢性阻塞性肺疾病、尘肺患者生活质量及其影响因素分析[J]. 中国医科大学学报, 2018, 47(6): 542-547.
[5] 朱春明, 张鹤, 郑锐. 中性粒细胞/淋巴细胞比值、红细胞分布宽度与慢性阻塞性肺疾病急性加重的相关分析[J]. 中国医科大学学报, 2018, 47(4): 312-315.
[6] 郭观华, 黄自明, 陈丽萍. 促血管生成microRNA在冠状动脉疾病诊断中的作用及其与疾病严重程度的关系[J]. 中国医科大学学报, 2018, 47(3): 268-274.
[7] 于婷婷, 张宁. 类风湿关节炎患者甲状腺相关抗体的临床分析[J]. 中国医科大学学报, 2018, 47(1): 32-35.
[8] 王思博,王慧彬,曲慧玲,赵珊珊,赵传胜. 卒中后抑郁的影响因素及其对生活质量的影响[J]. 中国医科大学学报, 2017, 46(9): 844-847.
[9] 杜守治 ,董斌 ,齐中华. 急性心肌梗死和急性脑梗死 miRNA 疾病标志物的初步筛查[J]. 中国医科大学学报, 2017, 46(8): 681-685.
[10] 于鹭,汤可娜,仲星星,李梅,管阳太. 视神经脊髓炎谱系疾病患者中水通道蛋白 4 抗体与视神经损害的相关性[J]. 中国医科大学学报, 2017, 46(4): 345-348.
[11] 赵辉,杨国峰. 综合护理措施在硫酸沙丁胺醇气雾剂吸入治疗慢性阻塞性肺疾病中的作用[J]. 中国医科大学学报, 2017, 46(2): 184-186.
[12] 蔡凯,刘志宇,王梁,戴志红,苏婕. 大连地区男性年龄相关前列腺特异性抗原参考值范围[J]. 中国医科大学学报, 2017, 46(10): 927-930.
[13] 沈力,盛楠,张莹. 学龄前期哮喘患儿照护者焦虑对疾病照护能力的影响[J]. 中国医科大学学报, 2017, 46(1): 41-44.
[14] 刘江伟,赵立,朱丽华,王娜,孙朋波. 气流受限不完全可逆的慢性阻塞性肺疾病患者容量和 流速变化与肺通气功能障碍严重程度分级的关系[J]. 中国医科大学学报, 2016, 45(9): 805-808.
[15] 赵琳,徐京京,王晓非,张晓莉. 类风湿关节炎合并牙周疾病患者的 IL-17A 表达[J]. 中国医科大学学报, 2016, 45(12): 1115-1118.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!

中国医科大学学报版权所有©2018

未经允许,严禁擅自转载本站图文资料

地址:中国 沈阳市沈北新区蒲河路77号 110122

辽ICP备05014850

JOURNAL OF CHINA MEDICAL UNIVERSITY

ADDRESS: NO.77 PUHE ROAD

SHENYANG NORTH NEW AREA, SHENYANG

LIAONING PROVINCE, P.R. CHINA