中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2013, Vol. 42 ›› Issue (7): 608–611.

• 论著 • 上一篇    下一篇

FK506对大鼠面神经损伤后面运动神经元凋亡及bcl-2,bax表达的影响

惠莲,刘凤啸,袁婧,姜学钧,任重   

  1. 中国医科大学附属第一医院耳鼻咽喉科,辽宁沈阳,110001
  • 收稿日期:2013-02-06 出版日期:2013-07-18 发布日期:2013-11-26
  • 作者简介:惠莲(1972-),女,博士,教授。
  • 基金资助:
    辽宁省自然科学基金(20072097)

Effects of FK506 on the Apoptosis and bcl-2, bax Expression of Rat Facial Motor Neurons after Facial Nerve Injury

HUI Lian1,LIU Feng-xiao1,YUAN Jing1,JIANG Xue-jun1,REN Zhong1   

  1. 1.Department of Otolaryngology, The First Hospital, China Medical University , Shenyang 110001, China
  • Received:2013-02-06 Online:2013-07-18 Published:2013-11-26

摘要: 目的研究FK506对大鼠面神经损伤后运动神经元凋亡及bcl-2及bax表达的影响。方法将40只Wistar大鼠随机分为实验组和对照组,制作大鼠单侧面神经总干切断模型,实验组每日腹腔注射FK506注射液,对照组给予相同剂量的盐水,在术后各时间点,通过Nissl染色观测面神经核神经元的形态学变化;TUNEL检测细胞凋亡;免疫组化检测bcl-2和bax表达的变化。结果面神经损伤后,FK506组与盐水组相比较,FK506组运动神经元凋亡明显减少,bcl-2表达增加,bax表达降低。结论FK506有助于增加面神经损伤后运动神经元bcl-2的表达、降低bax表达,抑制细胞凋亡,为药物在神经损伤疾病的临床应用提供了实验依据。

关键词: FK506, 面神经, 细胞凋亡, bcl-2, bax

Abstract: Objective To investigate effects of FK506 on the apoptosis and the expression of bcl-2 and bax of rat facial motor neurons after facial nerve injury. Methods A total of 40 Wistar rats were randomly divided into experimental and control group. Facial nerve injury model was established by transecting facial nerves at its stylomastoid foramen,and then the experimental group and the control group were treated with FK506(FK506-treated group) and normal saline(saline control group)by intraperitoneal injection, respectively .The morphology of facial neurons were observed under light microscope at different time points after injury;the apoptotic cells of injured FMNs were detected by TUNEL staining;the expression of bcl-2 and bax were evaluated by immunohistochemistry method. Results After facial nerve transection, the apoptotic cells were significantly decreased in FK506-treated group compared with saline control group (P<0.05) .The expression level of bcl-2 in FK506-treated group were higher than that in saline control group ,and the expression level of bax in FK506-treated group were lower than the control group . Conclusion FK506 could increase the expression of bcl-2, and decrease the expression of bax, and inhibit the apoptosis after facial nerve transection .The present results provide an experimental basis for the clinical application of drugs in nerve injury diseases.

Key words: FK506, facial nerve, apoptosis, bcl-2, bax

中图分类号: 

  • R745.12
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