中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2013, Vol. 42 ›› Issue (7): 612–614.

• 论著 • 上一篇    下一篇

阿托伐他汀抑制血管平滑肌细胞Cyr61的表达

付鹏,刘晓霞   

  1. 中国医科大学附属盛京医院心内科,沈阳110004
  • 收稿日期:2013-04-09 出版日期:2013-07-18 发布日期:2013-11-26
  • 作者简介:付鹏(1972-),女,副教授,博士.
  • 基金资助:
    辽宁省教育厅高校科研计划(L2010611)

Atorvastatin Inhibits Cysteine-rich 61 Expression in Cultured Rat Vascular Smooth Muscle Cells

FU Peng,LIU Xiao-xia   

  1. Department of Cardiology, Shengjing Hospital, China Medical University, Shenyang 110022, China
  • Received:2013-04-09 Online:2013-07-18 Published:2013-11-26

摘要: 目的探讨阿托伐他汀对增殖的血管平滑肌细胞Cyr61的作用。方法贴块法培养大鼠胸主动脉血管平滑肌细胞,将培养的平滑肌细胞分为正常对照组、血管紧张素#x02161;(Ang#x02161;)组和阿托伐他汀组。Ang#x02161;组将1#x003bc;mol/LAng#x02161;加入平滑肌细胞培养基,共培养180min;阿托伐他汀组在Ang#x02161;刺激细胞前1h加入10#x003bc;mol/L阿托伐他汀到培养基中。采用RT-PCR及Westernblot检测各组中各时点Cyr61mRNA和蛋白表达。结果Ang#x02161;组与正常对照组相比上调增殖的平滑肌细胞Cyr61mRNA和蛋白表达,阿托伐他汀组与Ang#x02161;组相比抑制Ang#x02161;刺激的Cyr61蛋白表达,尤其在30min时点更加明显(P<0.01)。结论阿托伐他汀抑制增殖的平滑肌细胞Cyr61的表达,为阿托伐他汀降脂外的抗动脉硬化作用增加新的理论依据。

关键词: 阿托伐他汀, 血管紧张素Ⅱ, Cyr61, 大鼠, 血管平滑肌细胞

Abstract: Objective To investigate the effect of atorvastatin on Cyr61expression in cultured rat vascular smooth muscle cells (VSMCs). Methods The VSMCs were isolated using mass adhesion method. VSMCs were divided into normal control group, Ang#x02161; group (VSMCs were treated with Ang#x02161;for 15 min, 30 min, 60 min and 180 min), and atorvastatin group (pretreated with atorvastatin for 60 min before Ang#x02161; treated). The Cyr61expression in VSMCs was characterized by RT-PCR and Western blot. Results Cyr61mRNA and protein expression in the proliferative smooth muscle cells was up-regulated by Ang#x02161;, and the Ang #x02161;-induced effect was impaired on atorvastatin group, especially at 30 min time points (there was a significant difference, P<0.01). Conclusion Atorvastatin can inhibit Cyr61protein expression in proliferative smooth muscle cells, which provide new theoretical basis for anti-atherosclerosis of atorvastatin.

Key words: atorvastatin, angiotensin II, Cyr61, rat, vascular smooth muscle cells

中图分类号: 

  • R541.1
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