中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2013, Vol. 42 ›› Issue (7): 615–618.

• 论著 • 上一篇    下一篇

消炎痛对活化小胶质细胞MHC-#x02161;类分子和CD11b分子表达的影响

罗钢1,靳赢2,王金岩2   

  1. 中国医科大学1.附属第一医院儿科;
    2.基础医学院免疫学教研室,沈阳110001
  • 收稿日期:2013-05-06 出版日期:2013-07-18 发布日期:2013-11-26
  • 作者简介:罗钢(1972-),男,副主任医师,博士.
  • 基金资助:
    辽宁省自然科学基金(20102294)

The in vitro Effects of Indomethacin on the Expression of MHC Class #x02161; and CD11b Molecules

LUO Gang1,JIN Ying2,WANG Jin-yan2   

  1. 1. Department of Pediatrics, The First Hospital, China Medical University, Shenyang 110001, China;
    2.Department of Immunology, College of Basic Medical Science, China Medical University, Shenyang 110001, China
  • Received:2013-05-06 Online:2013-07-18 Published:2013-11-26

摘要: 目的探讨消炎痛对活化小胶质细胞免疫相关分子MHC-#x02161;类分子和CD11b分子表达的影响。方法脂多糖(LPS)联合干扰素#x003b3;(IFN-#x003b3;)激活小胶质细胞株BV-2的同时采用不同浓度(0.1、0.5、1mmol/L)的消炎痛进行干预,流式细胞仪检测表面分子MHC-#x02161;类分子和CD11b分子的表达,Griess法检测培养上清中一氧化氮(NO)的浓度。结果LPS联合IFN-#x003b3;激活BV-2细胞后MHC-#x02161;类分子和CD11b分子的表达上调,NO的生成增加;消炎痛以剂量依赖方式下调活化的BV-2细胞MHC-#x02161;类分子和CD11b分子的表达,抑制NO的生成。结论消炎痛下调BV-2细胞MHC-#x02161;类分子和CD11b分子的表达并抑制NO的生成,提示消炎痛可能通过降低小胶质细胞免疫相关分子表达抑制活化小胶质细胞的抗原提呈功能、吞噬杀伤及细胞毒效应。

关键词: 消炎痛, 小胶质细胞, MHC-Ⅱ, 类分子, CD11b分子, 一氧化氮

Abstract: Objective To investigate the effects and mechanisms of indomethacin on the antigen-presenting function and phagocytoic activity of the activated microglial cells. Methods The microglial cell line-BV-2 cells were treated with different dose of indomethacin, and the expression of MHC class #x02161; and CD11b molecules was evaluated at different time course by the flow cytometry technique. Saline-treated BV-2 cells were used as negative control, and BV-2 cells treated with combined lipopolysaccharide (LPS) and IFN-#x003b3; were adopted as a positive control. NO production of these treated BV-2 cells was detected by Griess assay. Results Expression of MHC class-#x02161; molecules and CD11b molecule on BV-2 cells was significantly decreased after the indomethacin treatment with a dose dependent manner. The production of NO was also inhibited by indomethacin in a dose dependent way. Conclusion Indomethacin can inhibit the activation of microglia by suppression of the expression of MHC-class #x02161; molecules, CD11b molecules and NO production. These results indicated that Indomethacin could inhibit the antigen-presenting function, phagocytoic activity and NO production of mciroglia.

Key words: indomethacin, microglial cells, MHC-Ⅱ, CD11b, nitric oxide

中图分类号: 

  • R392.12
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