中国医科大学学报

中国医科大学学报
  • 中文核心期刊
  • 中国科技核心期刊
  • 中国高校百佳科技期刊
  • BA、CA收录

中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (9): 807-811.doi: 10.12007/j.issn.0258-4646.2018.09.009

• 论著 • 上一篇    下一篇

miR-26a在溃疡性结肠炎中的表达及对自噬的影响

曹勇, 王东旭, 孙妍, 杨俊, 张营, 郑长清   

  1. 中国医科大学附属盛京医院第二消化内科, 沈阳 110004
  • 收稿日期:2018-04-12 出版日期:2018-09-30 发布日期:2018-09-08
  • 通讯作者: 郑长清 E-mail:zhengchangqing88@163.com
  • 作者简介:曹勇(1980-),女,主治医师,硕士.
  • 基金资助:
    辽宁省科学技术计划(2013225303)

miR-26a Expression in Ulcerative Colitis and Its Effect on Cell Autophagy

CAO Yong, WANG Dongxu, SUN Yan, YANG Jun, ZHANG Ying, ZHENG Changqing   

  1. Department of Gastroenterology, Shengjing Hospital, China Medical University, Shenyang 110004, China
  • Received:2018-04-12 Online:2018-09-30 Published:2018-09-08

摘要: 目的 探讨miR-26a在溃疡性结肠炎中的表达及其与自噬和凋亡的关系。方法 应用实时PCR检测活动期溃疡性结肠炎患者和正常对照者组织及血清中miR-26a的表达。应用miR-26a mimics/inhibitor过表达/敲低miR-26a在Caco-2细胞中的表达,应用流式细胞技术检测凋亡情况,应用Western blotting检测相关蛋白LC3-Ⅱ/LC3-Ⅰ及p62蛋白的表达。结果 与正常对照者相比,活动期溃疡性结肠炎患者组织中miR-26a呈高表达;过表达miR-26a后Caco-2细胞凋亡增加,自噬水平降低,敲低miR-26a后Caco-2细胞凋亡减少,自噬增加。结论 活动期溃疡性结肠炎组织中miR-26a高表达,且其高表达促进细胞凋亡并抑制自噬。

关键词: 溃疡性结肠炎, 自噬, 凋亡

Abstract: Objective To determine miR-26a expression in ulcerative colitis and its relationship with autophagy and apoptosis. Methods miR-26a expression in sera of patients with active ulcerative colitis and normal controls was detected using real-time polymerase chain reaction(PCR). miR-26a mimics/inhibitor were used to upregulate/downregulate miR-26a expression. Apoptosis of Caco-2 cells was detected using flow cytometry. LC3-Ⅱ/LC3-Ⅰand p62 expression was detected by Western blotting. Results miR-26a expression in patients with active ulcerative colitis was higher than in normal controls. Furthermore, miR-26a expression level correlated positively with autophagy and negatively with apoptosis. Conclusion miR-26a is highly expressed in patients with active ulcerative colitis, which promotes apoptosis and reduces autophagy.

Key words: ulcerative colitis, autophagy, apoptosis

中图分类号: 

  • R284.2
[1] ⅡDA T, ONODERA K, NAKASE H. Role of autophagy in the pathogenesis of inflammatory bowel disease[J]. World J Gastroenterol, 2017, 23(11):1944-1953. DOI:10.3748/wjg.v23.i11.1944.
[2] ZAHM AM, HAND NJ, TSOUCAS DM, et al. Rectal microRNAs are perturbed in pediatric inflammatory bowel disease of the colon[J]. J Crohns Colitis, 2014, 8(9):1108-1117. DOI:10.1016/j.crohns.2014.02.012.
[3] IBORRA M, BERNUZZI F, CORREALE C, et al. Identification of serum and tissue micro-RNA expression profiles in different stages of inflammatory bowel disease[J]. Clin Exp Immunol, 2013, 173(2):250-258. DOI:10.1111/cei.12104.
[4] CAO B, ZHOU X, MA J, et al. Role of miRNAs in inflammatory bowel disease[J]. Dig Dis Sci, 2017, 62(6):1426-1438. DOI:10.1007/s10620-017-4567-1.
[5] FAN Q, YANG L, ZHANG X, et al. The emerging role of exosomederived non-coding RNAs in cancer biology[J]. Cancer Lett, 2018, 414:107-115. DOI:10.1016/j.canlet.2017.10.040.
[6] VALERA E, SPENCER B, MOTT J, et al. MicroRNA-101 modulates autophagy and oligodendroglial alpha-synuclein accumulation in multiple system atrophy[J]. Front Mol Neurosci, 2017, 10:329. DOI:10.3389/fnmol.2017.00329.
[7] LIGUORI M, NUZZIELLO N, LICCIULLI F, et al. Combined microRNA and mRNA expression analysis in pediatric multiple sclerosis:an integrated approach to uncover novel pathogenic mechanisms of the disease[J]. Hum Mol Genet, 2018, 27(1):66-79. DOI:10.1093/hmg/ddx385.
[8] WU F, ZIKUSOKA M, TRINDADE A, et al. MicroRNAs are differentially expressed in ulcerative colitis and alter expression of macrophage inflammatory peptide-2 alpha[J]. Gastroenterology, 2008, 135(5):1624-1635. DOI:10.1053/j.gastro.2008.07.068.
[9] BLANDER JM. Death in the intestinal epithelium-basic biology and implications for inflammatory bowel disease[J]. FEBS J, 2016, 283(14):2720-2730. DOI:10.1111/febs.13771.
[10] ZHI X, TAO J, LI Z, et al. MiR-874 promotes intestinal barrier dysfunction through targeting AQP3 following intestinal ischemic injury[J]. FEBS Lett, 2014, 588(5):757-763. DOI:10.1016/j.febslet.2014.01.022.
[11] NIJHUIS A, BIANCHERI P, LEWIS A, et al. In Crohn's disease fibrosis-reduced expression of the miR-29 family enhances collagen expression in intestinal fibroblasts[J]. Clin Sci(Lond), 2014, 127(5):341-350. DOI:10.1042/CS20140048.
[12] SIMONE NL, SOULE BP, LY D, et al. Ionizing radiation-induced oxidative stress alters miRNA expression[J]. PLoS One, 2009, 4(7):e6377. DOI:10.1371/journal.pone.0006377.
[13] ZIDAR N, BOSTJANCIC E, JERALA M, et al. Down-regulation of microRNAs of the miR-200 family and up-regulation of Snail and Slug in inflammatory bowel diseases-hallmark of epithelial-mesenchymal transition[J]. J Cell Mol Med, 2016, 20(10):1813-1820. DOI:10.1111/jcmm.12869.
[14] SCHAEFER JS, ATTUMI T, OPEKUN AR, et al. MicroRNA signatures differentiate Crohn's disease from ulcerative colitis[J]. BMC Immunol, 2015, 16:5. DOI:10.1186/s12865-015-0069-0.
[15] ANGRIMAN I, SCARPA M, CASTAGLIUOLO I. Relationship between pouch microbiota and pouchitis following restorative proctocolectomy for ulcerative colitis[J]. World J Gastroenterol, 2014, 20(29):9665-9674. DOI:10.3748/wjg.v20.i29.9665.
[16] CAO SS. Epithelial ER stress in Crohn's disease and ulcerative colitis[J]. Inflamm Bowel, 2016, 22(4):984-993. DOI:10.1097/MIB.0000000000000660.
[17] KASER A, NIEDERREITER L, BLUMBERG RS. Genetically determined epithelial dysfunction and its consequences for microflora-host interactions[J]. Cell Mol Life Sci:CMLS, 2011, 68(22):3643-3649. DOI:10.1007/s00018-011-0827-y.
[18] BREST P, LAPAQUETTE P, SOUIDI M, et al. A synonymous variant in IRGM alters a binding site for miR-196 and causes deregulation of IRGM-dependent xenophagy in Crohn's disease[J]. Nat Genet, 2011, 43(3):242-245. DOI:10.1038/ng.762.
[19] LACHER M, SCHROEPF S, BALLAUFF A, et al. Autophagy 16-like 1 rs2241880 G allele is associated with Crohn's disease in German children[J]. Acta Paediatr, 2009, 98(11):1835-1840. DOI:10.1111/j.1651-2227.2009.01438.x.
[20] ZHANG J, CHEN J, GU J, et al. Association of IL23R and ATG16L1 with susceptibility of Crohn's disease in Chinese population[J]. Scand J Gastroenterol, 2014, 49(10):1201-1206. DOI:10.3109/00365521.2014.936031.
[21] YANG SK, YE BD, SONG K. ATG16L1 contributes to Crohn's disease susceptibility in Koreans:overmuch concern for ethnic difference?[J]. Gut, 2015, 64(4):687-688. DOI:10.1136/gutjnl-2014-308242.
[22] PAIVA NM, PASCOAL LB, NEGREIROS LMV, et al. Ileal pouch of ulcerative colitis and familial adenomatous polyposis patients exhibit modulation of autophagy markers[J]. Sci Rep, 2018, 8(1):2619. DOI:10.1038/s41598-018-20938-5.
[1] 龙哲, 王旭, 侯伟建, 杨丹. 工频电磁场对小鼠海马神经元细胞系HT22增殖、凋亡及磷酸化JNK、ERK表达的影响[J]. 中国医科大学学报, 2019, 48(9): 782-785,790.
[2] 段秋艳, 杜秋红, 宋文玲, 苏嘉利, 徐萍. 血清sTWEAK和MMP-9在急性ST段抬高型心肌梗死患者中的表达及其与预后的关系[J]. 中国医科大学学报, 2019, 48(9): 833-836,851.
[3] 马瑞肖, 张慧杰, 李馨慧, 张淑兰. 谷氨酰胺缺乏对宫颈癌Hela细胞增殖、迁移和凋亡的影响及其机制[J]. 中国医科大学学报, 2019, 48(8): 709-713.
[4] 孙大壮, 宋春青, 许勇民, 董雪松. 丝裂原活化蛋白激酶通路在百草枯诱导人肺Ⅱ型上皮样细胞A549凋亡中的作用[J]. 中国医科大学学报, 2019, 48(7): 577-581.
[5] 付雪岩, 王雅玮, 王文青, 吴刚. RNA干扰沉默水通道蛋白3基因对人肝癌细胞SMMC-7721增殖和凋亡的影响[J]. 中国医科大学学报, 2019, 48(7): 596-600.
[6] 张帆, 张娇, 李函容, 严肖啸, 赵江月. 表面外胚层敲除Mfn2基因对小鼠眼视网膜发育的影响[J]. 中国医科大学学报, 2019, 48(6): 481-484,494.
[7] 成冬, 郭磊, 姜天龙, 周仁义, 刘文博, 罗鹏, 梅润宏, 杨旭浩. 17β-雌二醇通过GPR30和PI3K/Akt信号通路影响ATDC5软骨细胞线粒体自噬的机制[J]. 中国医科大学学报, 2019, 48(4): 289-294.
[8] 庞天舒, 王岩, 左中夫, 任克, 刘学政. 白藜芦醇对碘海醇所致糖尿病大鼠肾损伤的作用及其机制[J]. 中国医科大学学报, 2019, 48(4): 309-314.
[9] 蔡存伟, 温媛媛, 李晓燕, 张丽娜. SIAH1核异位表达抑制乳腺癌细胞凋亡的作用机制[J]. 中国医科大学学报, 2019, 48(4): 328-332,337.
[10] 何波, 王文瑞, 李健, 王平. 蛋白酶体抑制剂MG132对膀胱癌细胞增殖及凋亡的影响[J]. 中国医科大学学报, 2019, 48(4): 349-353.
[11] 段睿, 焦奥, 张城硕, 林建贞, 石悦, 张佳林. 短肽FLPNF对地塞米松诱导的胰岛细胞凋亡的保护作用[J]. 中国医科大学学报, 2019, 48(3): 193-200.
[12] 黄碧莹, 刘洁, 钟鸣, 王珺婷, 王小玢, 康媛媛, 孙妍, 乔雪. LC3在成釉细胞瘤中的表达及意义[J]. 中国医科大学学报, 2019, 48(2): 101-104.
[13] 李岩, 张德巍, 杨大业. 那可丁对人肺癌细胞A549生物学功能的影响[J]. 中国医科大学学报, 2019, 48(1): 66-70.
[14] 刘仁杰, 刘俊杰, 张尧, 王婧瑶, 王一超, 张婧曦, 梁文吉, 赵雅宁, 李建民. 亚低温通过调控内质网应激激活自噬改善蛛网膜下腔出血大鼠早期脑损伤[J]. 中国医科大学学报, 2018, 47(9): 824-828,833.
[15] 岳媛, 范秋灵, 都姝妍, 远方, 徐莉, 李琳, 刘楠, 姜奕, 王力宁. 乌索酸对高糖诱导人系膜细胞损伤的作用[J]. 中国医科大学学报, 2018, 47(8): 682-686.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] 贺雪梅, 熊欣, 邹建中, 李发琪, 龚晓波. 超声消融剂量与生物学焦域特征[J]. 中国医科大学学报, 2009, 38(9): 654 .
[2] 谭明旗, 边明艳. HA14-1诱导小鼠Lewis肺癌细胞凋亡的实验研究[J]. 中国医科大学学报, 2009, 38(9): 668 .
[3] 尹玉, 赵卫华, 胡健. 替米沙坦与非诺贝特对高脂饮食大鼠血清脂联素水平的影响[J]. 中国医科大学学报, 2009, 38(9): 674 .
[4] 郭哲, 徐冰, 卢利. 下颌骨髁突骨折治疗的Meta分析[J]. 中国医科大学学报, 2009, 38(9): 709 .
[5] 夏永辉, 徐克. 对吻式支架置入术治疗Leriche综合征15例体会[J]. 中国医科大学学报, 2009, 38(9): 714 .
[6] 谭明旗, 边明艳. HA14-1对环磷酰胺治疗小鼠Lewis肺癌增敏作用的实验研究[J]. 中国医科大学学报, 2009, 38(10): 737 .
[7] 张丽, 齐瑞群, 孙艳, 陈国红, 任宏伟, 陈洪铎, 高兴华. 卡介菌多糖核酸对特应性皮炎外周血CLA~+T细胞表达不同细胞因子的影响[J]. 中国医科大学学报, 2009, 38(10): 764 .
[8] 蔡鑫泽, 顾卉, 刘彤, 李丰. hLMO1编码基因重组质粒的构建及蛋白的表达和定位[J]. 中国医科大学学报, 2009, 38(11): 816 .
[9] 张华, 王秋月, 侯刚, 许惟元, 李猛, 康健. 辽宁部分农村地区居民吸烟情况与COPD患病关系的调查[J]. 中国医科大学学报, 2009, 38(11): 855 .
[10] 赵宁, 惠莲, 石林, 李巍, 姜学钧. 语前聋儿童人工耳蜗植入术后疗效分析[J]. 中国医科大学学报, 2009, 38(11): 862 .

中国医科大学学报版权所有©2018

未经允许,严禁擅自转载本站图文资料

地址:中国 沈阳市沈北新区蒲河路77号 110122

辽ICP备05014850

JOURNAL OF CHINA MEDICAL UNIVERSITY

ADDRESS: NO.77 PUHE ROAD

SHENYANG NORTH NEW AREA, SHENYANG

LIAONING PROVINCE, P.R. CHINA