中国医科大学学报

中国医科大学学报
  • 中文核心期刊
  • 中国科技核心期刊
  • 中国高校百佳科技期刊
  • BA、CA收录

中国医科大学学报 ›› 2019, Vol. 48 ›› Issue (1): 71-74.doi: 10.12007/j.issn.0258-4646.2019.01.015

• 论著 • 上一篇    下一篇

miR-24对肺癌生物学功能的抑制作用

王黎明, 杨大业, 仇剑, 张德巍   

  1. 中国医科大学附属第四医院第三普通外科, 沈阳 110032
  • 收稿日期:2017-11-14 出版日期:2019-01-30 发布日期:2019-01-05
  • 通讯作者: 张德巍 E-mail:421310907@qq.com
  • 作者简介:王黎明(1985-),男,医师,硕士.

miR-24 Inhibits Lung Cancer Cell Proliferation and Migration

Wang Liming, Yang Daye, Qiu Jian, Zhang Dewei   

  1. Third general surgery Department, The Fourth Affiliated Hospital, China Medical University, shenyang 110032, China
  • Received:2017-11-14 Online:2019-01-30 Published:2019-01-05

摘要: 目的 探讨miR-24调节肺癌发生发展的机制。方法 实时PCR检测30例肺癌组织中miR-24的含量,分析miR-24与患者生存期的关系。肺癌细胞系A549细胞中分别过表达或沉默miR-24后,通过MTT实验观察miR-24对其增殖的影响。Transwell实验观察miR-24对其迁移能力的影响。Western blotting检测miR-24对于A549细胞中增殖和迁移相关蛋白(CDK4/6、MMP2)的影响。结果 生存分析发现,miR-24表达低的患者5年生存率较低。MTT检测显示,miR-24过表达后可以显著抑制A549细胞的增殖,反之,当miR-24受到抑制后A549细胞的增殖受到促进。Transwell实验显示,miR-24过表达后可以显著抑制A549细胞的迁移,反之,当miR-24受到抑制后A549细胞的迁移受到促进。Western blotting检测结果显示,过表达miR-24可以抑制A549细胞中CDK4/6和MMP2的表达,miR-24受到抑制后A549细胞中CDK4/6和MMP2的表达受到促进。结论 miR-24可以抑制肺癌细胞的增殖和迁移。

关键词: miR-24, 肺癌, A549, 增殖, 迁移

Abstract: Objective To investigate how microRNA 24 (miR-24) regulates the development of lung cancer. Methods The levels of miR-24 in 30 cases of lung cancer were detected using real-time PCR,and the relationship between miR-24 levels and overall survival was analyzed. After overexpression or silencing of miR-24 in A549 lung cancer cells,the effect on cell proliferation was observed by the MTT assay. Transwell assays were carried out to observe the effect of miR-24 on cell migration. The effects of miR-24 on the expression of cyclin-dependent kinase (CDK) 4/6 and matrix metalloproteinase (MMP) 2 in A549 cells were examined by Western blotting. Results Survival analysis showed that patients with low miR-24 expression had a shorter survival time. The MTT-based viability assay revealed that overexpression of miR-24 inhibited A549 cell proliferation. Cell proliferation was promoted when miR-24 was inhibited. In the Transwell assay,overexpression of miR-24 significantly inhibited the A549 cell migration,and cell migration increased when miR-24 was inhibited. Western blotting analysis revealed that overexpression of miR-24 could inhibit CDK4/6 and MMP2 production in A549 cells. Inhibition of miR-24 was associated with increased production of CDK4/6 and MMP2 in A549 cells. Conclusion miR-24 can inhibit the proliferation and migration of A549 lung cancer cells.

Key words: miR-24, lung cancer, A549, proliferation, migration

中图分类号: 

  • R739.5
[1] ZHANG Z,YANG Y,ZHANG X. MiR-770 inhibits tumorigenesis and EMT by targeting JMJD6 and regulating WNT/β-catenin pathway in non-small cell lung cancer[J]. Life Sci,2017,188:163-171. DOI:10.1016/j.lfs.2017.09.002.
[2] TIAN Z,WEN S,ZHANG Y,et al. Identification of dysregulated long non-coding RNAs/microRNAs/mRNAs in TNM I stage lung adenocarcinoma[J]. Oncotarget,2017,8(31):51703-51718. DOI:10.18632/oncotarget.18512.
[3] LI H,JIANG Z,LENG Q,et al. A prediction model for distinguishing lung squamous cell carcinoma from adenocarcinoma[J]. Oncotarget,2017,8(31):50704-50714. DOI:10.18632/oncotarget.17038.
[4] PAVEL AB,CAMPBELL JD,LIU G,et al. Alterations in bronchial airway microRNA expression for lung cancer detection[J]. Cancer Prev Res (Phila),2017,10(11):651-659. DOI:10.1158/1940-6207.CAPR-17-0098.
[5] BU W,LUO T. miR-1297 promotes cell proliferation of non-small cell lung cancer cells:involving in PTEN/Akt/Skp2 signaling pathway[J]. DNA Cell Biol,2017,36(11):976-982. DOI:10.1089/dna.2017.3886.
[6] CUI S,LIAO X,YE C,et al. ING5 suppresses breast cancer progression and is regulated by miR-24[J]. Mol Cancer,2017,16(1):89. DOI:10.1186/s12943-017-0658-z.
[7] YUAN Y,KLUIVER J,KOERTS J,et al. miR-24-3p is overexpressed in hodgkin lymphoma and protects hodgkin and reed-sternberg cells from apoptosis[J]. Am J Pathol,2017,187(6):1343-1355. DOI:10.1016/j.ajpath.2017.02.016.
[8] ZHAO J,CHI J,GAO M,et al. Loss of PTEN expression is associated with high microRNA 24 level and poor prognosis in patients with tongue squamous cell carcinoma[J]. J Oral Maxillofac Surg,2017,75(7):1449. DOI:10.1016/j.joms.2017.03.025.
[9] CHO S,WU CJ,NGUYEN DT,et al. A novel miR-24-TCF1 Axis in modulating effector T cell responses[J]. J Immunol,2017,198(10):3919-3926. DOI:10.4049/jimmunol.1601404.
[10] LIN Y,GU Q,SUN Z,et al. Upregulation of miR-3607 promotes lung adenocarcinoma proliferation by suppressing APC expression[J]. Biomed Pharmacother,2017,95:497-503. DOI:10.1016/j.biopha.2017.08.052.
[11] THEAN LF,WONG YH,LO M,et al. Chromosome 19q13 disruption alters expressions of CYP2A7,MIA and MIA-RAB4B lncRNA and contributes to FAP-like phenotype in APC mutation-negative familial colorectal cancer patients[J]. PLoS One,2017,12(3):e0173772. DOI:10.1371/journal.pone.0173772.
[12] KANG H,RHO JG,KIM C,et al. The miR-24-3p/p130Cas:a novel axis regulating the migration and invasion of cancer cells[J]. Sci Rep,2017,7:44847. DOI:10.1038/srep44847.
[13] LIU Z,LIU Z,ZHANG Y,et al. miR-24 represses metastasis of human osteosarcoma cells by targeting Ack1 via AKT/MMPs pathway[J]. Biochem Biophys Res Commun,2017,486(2):211-217. DOI:10.1016/j.bbrc.2017.02.045.
[14] HASHIMOTO Y,SHⅡNA M,KATO T,et al. The role of miR-24 as a race related genetic factor in prostate cancer[J]. Oncotarget,2017,8(10):16581-16593. DOI:10.18632/oncotarget.15016.
[1] 白云鹏, 许顺. 培美曲塞联合铂类一线药物治疗晚期非鳞型非小细胞肺癌的疗效[J]. 中国医科大学学报, 2019, 48(5): 469-471.
[2] 王子文, 刘兆玉. miR-510通过打靶c-MYC抑制肝细胞癌的发展[J]. 中国医科大学学报, 2019, 48(4): 300-304.
[3] 陈菁, 刘旭, 杨硕, 罗光伟. 经支气管镜针吸活检联合多标志物检测在肺癌病理分型中的应用价值[J]. 中国医科大学学报, 2019, 48(4): 342-345.
[4] 何波, 王文瑞, 李健, 王平. 蛋白酶体抑制剂MG132对膀胱癌细胞增殖及凋亡的影响[J]. 中国医科大学学报, 2019, 48(4): 349-353.
[5] 高天, 余铃, 李舒, 刘佳勇, 白楚杰, 薛瑞峰, 张路, 方志伟, 樊征夫. Notch信号通路促进滑膜肉瘤细胞SW982增殖和侵袭的研究[J]. 中国医科大学学报, 2019, 48(3): 210-215.
[6] 赵越, 孙媛媛, 佟雪, 岳鹏杰, 富伟能. miR-362-3p在喉癌组织中的表达及其对喉癌Hep-2细胞迁移的影响[J]. 中国医科大学学报, 2019, 48(3): 236-239.
[7] 姜雪梅, 钱越, 戴红良. 染料木黄酮通过ERK及JNK通路抑制人非小细胞肺癌PC14细胞增殖[J]. 中国医科大学学报, 2019, 48(3): 274-276,280.
[8] 樊璐, 王慧, 肖庆桓. 跨膜蛋白16F对乳腺癌细胞增殖及迁移的影响[J]. 中国医科大学学报, 2019, 48(2): 124-127.
[9] 温行, 李章富, 王辉, 孙绍华, 郭星, 李福才. miR-200c调控肽基脯氨酰顺反异构酶对喉癌Hep-2细胞生物学行为的影响[J]. 中国医科大学学报, 2019, 48(1): 17-22,28.
[10] 李岩, 张德巍, 杨大业. 那可丁对人肺癌细胞A549生物学功能的影响[J]. 中国医科大学学报, 2019, 48(1): 66-70.
[11] 王倩文, 徐振华, 王志静, 苏荣健, 陈学军, 谷艳娇. 葡萄糖调节蛋白78对L858R突变的非小细胞肺癌erlotinib敏感性的影响[J]. 中国医科大学学报, 2018, 47(8): 701-704.
[12] 管超, 于莉, 徐兆国, 崔国元, 张晓晔. 小细胞肺癌免疫治疗的研究进展[J]. 中国医科大学学报, 2018, 47(8): 740-743,747.
[13] 石岱旺, 徐然, 韩云, 石文君. 单孔与三孔胸腔镜肺叶切除术治疗周围型肺癌的临床疗效比较[J]. 中国医科大学学报, 2018, 47(7): 609-611,616.
[14] 宋起滨, 王哲, 王宇令, 李杨, 林爽. 脂肪源性干细胞促进大鼠神经施万细胞增殖和分泌功能[J]. 中国医科大学学报, 2018, 47(6): 527-531.
[15] 李忠华, 李慧峰, 李晓蕾. 瞬时感受器电位香草酸受体3促进胶质瘤U251细胞系增殖和侵袭[J]. 中国医科大学学报, 2018, 47(5): 394-397.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] 黄波, 李强, 张景国, 韩晓光, 刘君, 何莲, 刘也夫. 胆囊腺瘤癌变过程及免疫表型P53的诊断价值[J]. 中国医科大学学报, 2009, 38(9): 703 .
[2] 孟洁, 邓娓娓. 普通体检对233例中老年代谢综合征患者各项指标转归的影响[J]. 中国医科大学学报, 2009, 38(11): 876 .
[3] 马秀岚, 李巍, 季文樾, 王宏伟, 边志刚, 顾兆伟. 先天性舌根囊肿的临床分析[J]. 中国医科大学学报, 2009, 38(12): 943 .
[4] 于雪馨, 王常林, 孙荣国, 杨屹. 鼠输尿管完全梗阻后肾脏病理及足细胞表型改变的研究[J]. 中国医科大学学报, 2010, 39(1): 1 .
[5] 王新利, 李卿, 高婷, 付立叶, 王扬, 蒋涛, 姜又红. 树突状细胞分泌的exosomes体外诱导特异性CTL抗肾癌的初步研究[J]. 中国医科大学学报, 2010, 39(1): 4 .
[6] 刁延娜, 马兰兰, 孟凡彪, 李宏图, 庞希宁. 骨髓间充质干细胞移植促进大鼠损伤脊髓的功能恢复[J]. 中国医科大学学报, 2010, 39(1): 7 .
[7] 张依宁, 魏敏杰, 孙明军. 伊立替康抑制人结直肠癌细胞增殖和ATP敏感性钾通道的相关性研究[J]. 中国医科大学学报, 2010, 39(1): 10 .
[8] 赵宇, 谢鹏, 朱晓峰, 蔡志友. 大鼠神经干细胞中MAPKK/MEK-MAPK/ERK信号转导通路的实验研究[J]. 中国医科大学学报, 2010, 39(1): 14 .
[9] 夏蒲, 徐小燕, 贾宝萍, 王薇, 关一夫, 高野康雄, 郑华川. 胃黏膜特异表达JC病毒T抗原质粒构建及表达鉴定[J]. 中国医科大学学报, 2010, 39(1): 18 .
[10] 黄海能, 罗起胜, 赵邦, 谭源福, 邓元央, 黄华东, 符黄德, 李传玉. 兔脑创伤后早期血清β淀粉样前体蛋白动态变化的实验研究[J]. 中国医科大学学报, 2010, 39(1): 22 .

中国医科大学学报版权所有©2018

未经允许,严禁擅自转载本站图文资料

地址:中国 沈阳市沈北新区蒲河路77号 110122

辽ICP备05014850

JOURNAL OF CHINA MEDICAL UNIVERSITY

ADDRESS: NO.77 PUHE ROAD

SHENYANG NORTH NEW AREA, SHENYANG

LIAONING PROVINCE, P.R. CHINA