Journal of China Medical University

Journal of China Medical University
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Journal of China Medical University ›› 2015, Vol. 44 ›› Issue (6): 562-564.

• Orignal Article • Previous Articles     Next Articles

The Effect of Myeloid Differentiation Factor 88 Inhibitor ST2825 on the Autophagy of THP?1 Cells Infected with Recombinant Mycobacterium smegmatis

HU Shao-ting,LI Sheng-jin,HUANG Qin   

  1. Department of Respiratory Medicine,The Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China
  • Received:2015-01-03 Online:2015-06-20 Published:2015-06-19

Abstract: Objective To investigate the effect of myeloid differentiation factor 88 inhibitor ST2825 on the autophagy of THP?1 cells infected by recombinant mycobacterium smegmatis. Methods The myeloid differentiation factor 88 inhibitor ST2825 was applied on the THP?1 cells infected by recombinant mycobacterium smegmatis,and three groups were defined:the test group with ST2825 treatment,the control group without ST2825 treatment,and the blank group. Autophagosomes were observed under the fluorescence microscope,and the mRNA expression of Beclin?1 gene and Bcl?2 gene was analyzed by RT?PCR. Results Compared with the control group,the number of autophagy fluorescent dots in the test group was obviously reduced(P < 0. 05),and the expression levels of Beclin 1 gene and Bcl?2 gene were declined as indicated by the RT?PCR detection. Conclusion The myeloid differentiation factor 88 inhibitor ST2825 might inhibit the autophagy of THP?1 cells through interfering the separation of Beclin?1 and Bcl?2.

Key words: myeloid differentiation factor 88, ST2825, recombinant mycobacterium smegmatis

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(编辑 武玉欣)
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[2] SUN Dandan,WU Yupeng,LIU Wen,YAN Hong,WANG Honghu,YANG Jun. Relationship of MyD88 rs7744 Polymorphism with the Risk and Severity of Coronary Artery Disease [J]. Journal of China Medical University, 2017, 46(6): 519-523.
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